Bioaktivne komponente u funkciji oplemenjivanja industrijskog paradajza

Selection of tomato lines for industrial use regarding their nutritional value is a potential that will reflect in the final product obtained from tomato fruits. At the same time, technological process of preparation of tomato juice (100 °C), as well as hot air drying, was studied in order to establish an optimal technological process that is the least disrupting for the natural potential of nutrients contained in fresh fruits. The research was performed on 6 lines and one variety of industrial tomato. The content of vitamin C, vitamin E, lycopene, β-carotene, phenols, flavonoids, dry matter and total antioxidative capacity was studied by applying standard methods for determination of the level of these parameters. Grouping was performed according to traits of average carotenoid content (lycopene, β-carotene), vitamin C, vitamin E, phenols, flavonoids, dry matter and total antioxidative capacity in tomato fruit, by applying PCA. The first two principle components were responsible for 77.18 % of total variability of researched samples. The impact of other five components was low and they were responsible for 22.18 % of the variability. Antioxidative activity was best preserved after finishing, through small losses of lycopene and β-carotene. Losses of vitamin C, vitamin E, phenols and flavonoids during thermal processing were great, mostly in juice, while in dried product, these losses were lower. Nutrient losses were in function of temperature height that the fruits were exposed to during the thermal processing. Genotypes SPRZ and SPSM were marked as the best.Selekcija linija paradajza namenjenog industrijskoj preradi prema osobinama koje definišu njihovu nutritivnu vrednost predstavljaju potencijal koji će se reflektovati u proizvodu dobijenom od plodova paradajza. U isto vreme ispitan je i tehnološki proces pripreme soka paradajza (100 °C) kao i sušenje plodova na toplom vazduhu, kako bi se utvrdio optimalni tehnološki proces koji najmanje narušava prirodni potencijal nutrijenata sadržanih u svežim plodovima. Ispitivanje je sprovedeno na 6 linija i jednoj sorti industrijskog paradajza gde su ispitani sadržaj vitamina C, vitamina E, likopina, β-carotina, fenola, flavonoida, suve materije i ukupni antioksidativni kapacitet, standardnim metodama za utvrđivanje sadržaja ovih parametara. Grupisanje je izvršeno prema osobinama prosečnog sadržaja karetinoida (likopen, β-karoten), vitamina C, vitamina E, fenola, flavonoida, suve materije i ukupnog antioksidatinog kapaciteta u plodu paradajza, pomoću PCA analize. Prve dve principle komponente odgovorne su za 77,18 % ukupne varijabilnosti ispitivanih uzoraka. Uticaj ostalih pet komponenti je mali i one su odgovorne za 22,18 % varijabilnosti. Antioksidativna aktivnost je najbolje očuvana posle dorade kroz male gubitke likopina i β-karotina posle dorade. Gubici vitamina C, vitamina E, fenola, flavonoida pri termičkoj obradi su veliki i to najviše u soku, dok kod sušenog proizvoda ti gubici su nešto manji. Gubici nutrijenata su u funkciji visine temperature kojima su plodovi bili izlagani pri termičkoj obradi. Kao najbolji genotipovi ocenjeni su SPRZ i SPSM

Uticaj termičke obrade plodova paradajza na antioksidativnu aktivnost, sadržaj vitamina E, suve materije i ukupnih šećera

The objective of this study was the determination of total antioxidant activity, contents of vitamin E, sugar and total dry matter in fresh and thermally processed (dried and juiced) tomato fruits of different selected tomato lines with the aim to establish the nutritive profile and distinguish superior genotypes in order to obtain high-quality final product with more benefit to human health. Content of vitamin E, total antioxidant activity, dry matter and total sugars, in fresh and dried fruits (dried in parallel hot air dryer at 60 °C, until the final product reached the moisture lower than 10% and in tomato juice pasteurized at 100 °C, for 7 minutes) was studied. Comparative trial with 7 genotypes: one commercial variety (SP-109) and 6 selected high inbreeding generation lines (SPP, SPSM, SPRZ, SPRM-20, S-60 and SPO), was set up. Genotype SPRZ had the highest vitamin E content and total antioxidant capacity, both in fresh fruit and after the treatments. Thermal processing by drying at 60°C and pasteurization of tomato changed the level of total sugar and dry matter content. Total antioxidant activity decreased by drying, comparing to fresh fruit while the level of vitamin E decreased in juice pasteurized at high temperature (100 °C). .Cilj istraživanja je određivanje ukupne antioksidativne aktivnosti, sadržaja vitamina E, sadržaja šećera i ukupne suve materije u svežim i termički obrađenim (sušeni plodovi i sok) plodovima različitih selekcionih linija paradajza, sa ciljem utvrđivanja nutritivnog kvaliteta i izdvajanja superiornih genotipova, čijom preradom bi se dobio kvalitetniji krajnji proizvod sa pozitivnim dejstvom na ljudsko zdravlje. Ispitivani su sadržaj vitamina E, ukupna antioksidativna aktivnost, suva materija, i ukupni sadržaj šećera, kod svežih plodova, sušenih plodova (sušenje toplim vazduhom na 60 °C, do konačne vlage uzorka manje od 10%) i soka paradajza (pasterizacijom na 100 °C, u trajanju od 7 minuta). Izveden je komparativni ogled sa 7 genotipova, jedna komercijalna sorta (SP-109) i 6 selekcionisanih linija (SPP, SPSM, SPRZ, SPRM-20, S-60 I SPO) visokih generacija inbridinga. Termička obrada plodova paradajza (sušenjem na 60 °C) i pravljenjem soka dovodi do promene ukupnog sadržaja šećera i sadržaja ukupne suve materije. Ispitivanjem ukupne antioksidativne aktivnosti utvrđeno je da se ona gubi sušenjem u odnosu na svež plod, a sadržaj vitamina E se smanjio u soku koji je dobijen obradom na višim temperaturama (100 °C). Genotip SPRZ imao je najviše vrednosti za sadržaj vitamina E i ukupan antioksidativni kapacitet kako u svežem stanju tako i nakon primenjene obrade. Izborom superiornih genotipova i akumulacijom nutrijenata kroz oplemenjivačke programe, može se poboljšati kvalitet krajnjeg proizvoda i postići dobijanje prerađevina sa većim pozitivnim uticajem na zdravlje ljudi

Design of class 4 cross section in axial compression according to EUROCODE 3

In this paper theoretic basis of design of class 4 cross section in axial compression according to EN1993-1-5 is given. Design procedure is given as an flow chart and ilustrated with an worked example. Plate buckling is treated localy and globaly through the concept of effective cross sections. Interaction between plate-like and column-like behaviour of panels is carried out thought buckling reduction factors and final effective area of cross section

Rezultati oplemenjivanja salatnog krastavca (Cucumis sativus L.) u Institutu za povrtarstvo, Smederevska Palanka

Market significance of cucumber for fresh consumption and the new technology of growing make it one of the most attractive vegetable variety. Interest of producers is higher every day, while the selection of new varieties is very low. The aim of this research was the selection of cucumber intended for fresh consumption with good agronomic traits intended for growing in the greenhouses and in the open field. The researchers of the Institute for Vegetable Crops, Smederevska Palanka created new cucumber hybrid which was recognized by Ministry of Agriculture Forestry and Water Management of the Republic of Serbia, Department for Plant protection in 2013, under the name 'Kralj'.Tržišna značajnost salatnog krastavca i nove tehnologije gajenja čine da on postaje jedna od najatraktivnijih povrtarskih vrsta. Zainteresovanost proizvođača je sve veća a domaća selekcija sorata na ovoj povrtarskoj vrsti je jako oskudna. Cilj ovog istraživanja je selekcija salatnog krastavca sa dobrim agronomskim osobinama namenjenog za gajenje u zaštićenom prostoru i na otvorenom polju. Novi hibrid salatnog krastavca Kralj, kreiran je u Institutu za povrtarstvo, Smederevska Palanka. Hibrid je priznat kod MPVŠ, RS, Uprava za zaštitu bilja, 2013 godine pod nazivom Kralj

Wind towers - design of flange ring connection

Today, wind power is second largest available renewable resource of energy, with 870 TW (terawatts). Large wind farms, with hundreds of wind towers are big opportunity for steel production industry. Designers and manufacturers of wind towers pay special attention on every single detail, always looking for some new solutions, trying to reduce price of wind towers. Some of the most interesting design and manufacturing details are connections used to assemble sections of tubular steel towers supporting wind turbins. There is traditional and actually more used flange ring connection and on the other side, as a new proposal which is used more and more, friction connection. In this paper theoretic basis of design of flange ring connection of wind towers is give

Bioinformatics analysis of SARS coronavirus genome polymorphism

BACKGROUND: We have compared 38 isolates of the SARS-CoV complete genome. The main goal was twofold: first, to analyze and compare nucleotide sequences and to identify positions of single nucleotide polymorphism (SNP), insertions and deletions, and second, to group them according to sequence similarity, eventually pointing to phylogeny of SARS-CoV isolates. The comparison is based on genome polymorphism such as insertions or deletions and the number and positions of SNPs. RESULTS: The nucleotide structure of all 38 isolates is presented. Based on insertions and deletions and dissimilarity due to SNPs, the dataset of all the isolates has been qualitatively classified into three groups each having their own subgroups. These are the A-group with "regular" isolates (no insertions / deletions except for 5' and 3' ends), the B-group of isolates with "long insertions", and the C-group of isolates with "many individual" insertions and deletions. The isolate with the smallest average number of SNPs, compared to other isolates, has been identified (TWH). The density distribution of SNPs, insertions and deletions for each group or subgroup, as well as cumulatively for all the isolates is also presented, along with the gene map for TWH. Since individual SNPs may have occurred at random, positions corresponding to multiple SNPs (occurring in two or more isolates) are identified and presented. This result revises some previous results of a similar type. Amino acid changes caused by multiple SNPs are also identified (for the annotated sequences, as well as presupposed amino acid changes for non-annotated ones). Exact SNP positions for the isolates in each group or subgroup are presented. Finally, a phylogenetic tree for the SARS-CoV isolates has been produced using the CLUSTALW program, showing high compatibility with former qualitative classification. CONCLUSIONS: The comparative study of SARS-CoV isolates provides essential information for genome polymorphism, indication of strain differences and variants evolution. It may help with the development of effective treatment

Varijabilnost i koeficijent heritabilnosti prosečnog sadržaja suve materije u lukovicama crnog luka (Allium cepa L.)

In order to research the variability of average dry matter content in onion bulbs, the trial has been set up on the experimental plot in the Centre for Vegetable Crops in Smederevska Palanka during 2000. and 2001. The trial has been performed by applying the method of random block design with five replicas. Ten cultivars of different geographical origin have been used. Examined characteristics were determined by applying the variance analysis of two-factorial trial - model 2. Hadzivukovic, 1991. The components of phenotype variance, genotype and phenotype coefficient of variance and heritability in broader sense have been calculated according to SINGH and CHAUDHARY (1976). The significant variability has been found for dry matter content in bulbs during both years of research. The researched trait had a greater genotype variance than out door conditions factor variance and the coefficient of phenotype variation greater than genetic variance coefficient. High percentage of genetic variability in total phenotype variability has been confirmed by high heritability. .U cilju ispitivanja varijabilnosti prosečnog sadržaja suve materije u lukovicama crnog luka, postavljen je ogled na oglednom polju Centra za povrtarstvo u Smederevskoj Palanci u toku 2000. i 2001. godine. Ogled je izveden po slučajnom blok sistemu u pet ponavljanja. Za istraživački materijal korišćeno je deset sorata, različitog geografskog porekla. Utvrđene vrednosti ispitivanih svojstava su obrađene analizom varijanse dvofaktorijalnog ogleda-model 2. (Hadživuković, 1991). Komponente fenotipske varijanse, genotipski i fenotipski koeficijent varijacije i heritabilnost u širem smislu izračunate su prema Singh i Chaudhary (1976). Značajna varijabilnost je dobijena za prosečni sadržaj suve materije u lukovicama tokom obe godine istraživanja. Ispitivana osobina imala je varijansu genotipa veću od varijanse faktora spoljašnje sredine i koeficijent fenotipske varijacije veći od koeficijenta genetičke varijacije. Veći udeo genetičke u ukupnoj fenotipskoj varijabilnosti potvrđuje visoka heritabilnost.

Wind towers - design of flange ring connection

Today, wind power is second largest available renewable resource of energy, with 870 TW (terawatts). Large wind farms, with hundreds of wind towers are big opportunity for steel production industry. Designers and manufacturers of wind towers pay special attention on every single detail, always looking for some new solutions, trying to reduce price of wind towers. Some of the most interesting design and manufacturing details are connections used to assemble sections of tubular steel towers supporting wind turbins. There is traditional and actually more used flange ring connection and on the other side, as a new proposal which is used more and more, friction connection. In this paper theoretic basis of design of flange ring connection of wind towers is give

Design of class 4 cross section in axial compression according to EUROCODE 3

In this paper theoretic basis of design of class 4 cross section in axial compression according to EN1993-1-5 is given. Design procedure is given as an flow chart and ilustrated with an worked example. Plate buckling is treated localy and globaly through the concept of effective cross sections. Interaction between plate-like and column-like behaviour of panels is carried out thought buckling reduction factors and final effective area of cross section

Advanced hybrid composite coatings based on calcium phosphate on titanium for potential biomedical applications

This paper deals with the issue of defining a new method of anodizing/anaphoretic deposition for the application of calcium phosphate and hybrid coatings based on calcium phosphate ceramics on titanium and anodized titanium coatings with improved properties. Hybrid coatings consisted of chitosan oligolactate (ChOL) and ChOL with Se as immunomodulatory oligoelement. The paper contributes to solving the problem of multi-stage pre-treatment and posttreatment of titanium and oxidized titanium surface to obtain a coating on the substrate, adhesion of the coating, antimicrobial and cytotoxic properties that occur in biomaterials, as well as reduced immune inflammatory response of the organism. It primarily deals with the creation and optimization of a new in situ anodizing/anaphoretic deposition process for obtaining multifunctional composite biomaterials. The in situ method results in improved adhesion bioactive coating, increased bioactivity and biocompatibility with increased antimicrobial properties and absence of cytotoxicity. The studied biomaterials have improved properties such as: corrosion resistance, absence of toxicity to the human body and adequate strength, which enables their potential use in medicine and dentistry. The aim of the research was to define new in situ anodizing/anaphoretic deposition process and adequate modification of process parameters for application of composite calcium phosphate coatings on titanium and its alloys, wherein innovation is reflected in combining calcium phosphate coating synthesis and surface modification by partially incorporating a ceramic coating into the crystalline structure of the substrate. The characterization of the coatings obtained in this manner was performed by various physico-chemical, biochemical and biological methods. These characterization techniques included: AFM, SEM, FE-SEM, roughness testing, XRD, FTIR, bioactivity, biocompatibility, cytotoxicity, antimicrobial activity and in vivo testing