Double-Helix Microscopy for Wide-Field 3-D Single-Molecule Fluorescence Imaging

We present methods to improve the localization accuracy in wide-field 3-D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3-D localization, the estimation algorithms, and polarization sensitive detection

The Secure Data Sharing Mechanism for Cloud Computing Based Cloud Storage System

Secure cloud storage, which is an emerging cloud service, is designed to protect the confidentiality of outsourced data but also to provide flexible data access for cloud users whose data is out of physical control. The emergence of cloud infrastructure has significantly reduced the costs of hardware and software resources in computing infrastructure. To ensure security, the data is usually encrypted before it’s outsourced to the cloud. Unlike searching and sharing the plain data, it is challenging to search and share the data after encryption. Nevertheless, it is a critical task for6 the cloud service provider as the users expect the cloud to conduct a quick search and return the result without losing8 data confidentiality. To overcome these problems, Finally, the proposed construction21 is demonstrated practical and efficient in the performance and property comparison. However, the use of CP-ABE may yield an inevitable security breach which is known as the misuse of access credential (i.e. decryption rights), due to the intrinsic “all-or-nothing” decryption feature of CP-ABE. In this paper, we investigate the two main cases of access credential misuse: one is on the semi-trusted authority side, and the other is on the side of cloud user. To mitigate the misuse, We also present the security analysis and further demonstrate the utility of our system via experiments

Effect of Packaging Materials on Retention of Quality Characteristics of Dehydrated Green Leafy Vegetables during Storage

The objective of this study was to investigate the influence of blanching, dehydration and packaging on nutrient composition of Amaranthus gangeticus and Spinach oleracea. There was a loss of sugars, Proteins, vitamin- c and carotenoids were noticed due to blanching. But the colour of green leafy vegetables (GLV) is retained due to blanching. Leafy vegetables were dehydrated in cabinet dryer at 60oC and packed in three packaging materials (Metalized polypropylene (MPP) 300 gauge, high density polyethylene (HDPE) 300 gauge, low density polyethylene (LDPE) 200 gauge) and stored at room temperature for 45 days to evaluate the best package for maximum retention of nutrients in leafy vegetables during storage. PP followed by HDPE was found to be good for retention of nutrients in dehydrated leafy vegetables during 45 days of storage. Irrespective of the losses of nutrients that take place during dehydrated packaging, GLV can be preserved by dehydration which is eco-friendly and easily adoptable

An Adaptive Technique to Predict Heart Disease Using Hybrid Machine Learning Approach

cardiovascular disease is amongby far prevalent fatalities in today's society. Cardiovascular disease is extremely hard to predict using clinical data analysis. Machine learning (ML) hasproved to be useful for helping in judgement and predictions with the enormous amount data produced by the healthcare sectorbusiness. Furthermore, latest events in other IoT sectors have demonstrated that machine learning is used (IOT). Several studies have examined the use of MLa heart disease prediction. In this research, we describe a novel method that, by highlighting essential traits, can improvethe precision of heart disease prognosis. Numerous data combinations and well-known categorization algorithms are used to create the forecasting models. Using a decent accuracy of 88.7%, we raise the level of playusing a heart disease forecasting approach that incorporates a88.7% absolute certainty in a combination random forest and linear model. (HRFLM)

GREEN SYNTHESIS OF NANOSTRUCTURED ZINC PARTICLES USING AQUEOUS LEAF EXTRACT OF SCHREBERA SWIETENIOIDES ROXB. AND THEIR CATALYTIC APPLICATION IN DEGRADATION OF METHYL ORANGE, CRYSTAL VIOLET DYES AND CHROMIUM METAL

Objective: The present work was aimed to synthesized the zinc nanoparticles (ZnO NPs) using aqueous leaf extract of Schrebera swietenioides Roxb., and further, the green-synthesized ZnO NPs were studied for its efficacy in the degradation of hazardous dyes like methyl orange, crystal violet and hazardous metal such as chromium. Methods: The ZnO NPs were synthesized using aqueous leaf extract of S. swietenioides Roxb., as a green reducing agent and 0.1 M Zinc acetate as metal source and the NPs synthesis was completed within a short period of 6 h. The ZnO NPs synthesized were characterized using SEM, TEM, EDS, XRD, FT-IR and UV-visible spectrophotometer. Further, the synthesized NPs were applied for reduction of pollutant days such as methyl orange, crystal violet and pollutant metal chromium. Results: The synthesis of NPs was monitored by observing the color change in the reaction mixture and UV visible spectral analysis. The UV spectral analysis shows a characteristic absorption wavelength at 379 nm. The synthesized NPs were hexagonal wurtzite form crystals having a spherical shape with rough surfaces with an average size of 68 nm and having 73.7 % of zinc content. At a NPs dose of 1.0 g/l the photocatalytic reduction was observed as 85.33±0.02 %, 86.82±0.095 % and 86.73±0.104 % for crystal violet dye, methyl orange dye and chromium metal, respectively. The NPs shows a high % photocatalytic reduction of chromium metal, crystal violet dye and methyl orange dye with less contact time confirms that the synthesized ZnO NPs were effectively catalyzed the degradation of methyl orange, crystal violet dyes and chromium metal. The NPs were observed to be recyclable and can shows high reduction activity after the completion of three cycles of degradation. Conclusion: Hence it can be concluded that synthesized greener nanocatalyst was efficient for pollutant treatment and demonstrated the power of green biosynthesis for metallic nanoparticles

A BATTERY ENERGY STORAGE SYSTEM WITH CONTROLLED REDUCED-RATING DYNAMIC VOLTAGE RESTORER

In this paper, different voltage injection schemes for dynamic voltage restorers (DVRs) are analyzed with particular focus on a new method used to minimize the rating of the voltage source converter (VSC) used in DVR. A new control technique is proposed to control the capacitor-supported DVR. The control of a DVR is demonstrated with a reduced-rating VSC. The reference load voltage is estimated using the unit vectors. The synchronous reference frame theory is used for the conversion of voltages from rotating vectors to the stationary frame. The compensation of the voltage sag, swell, and harmonics is demonstrated using a reduced-rating DVR. Power quality problems such as transients, sags, swells, and other distortions to the sinusoidal waveform of the supply voltage affect the performance of these equipment pieces. Technologies such as custom power devices are emerged to provide protection against power quality problems. Custom power devices are mainly of three categories such as series-connected compensators known as dynamic voltage restorers (DVRs), shunt-connected compensators such as distribution static compensators, and a combination of series and shunt-connected compensators known as unified power quality conditioner. The DVR can regulate the load voltage from the problems such as sag, swell, and harmonics in the supply voltages. Hence, it can protect the critical consumer loads from tripping and consequent losses. The custom power devices are developed and installed at consumer point to meet the power quality standards such as IEEE-51

Experimental Study and Optimization of Machining Parameters in Turning of AISI 1040 Steel with Micro-grooved WC Cutting Tools

In dry turning, control over chip formation and the need for the automated machining lead to an advancement in cutting tools. Such concern towards chip breakability is necessary in reducing tool wear, tool tip temperatures and surface roughness of workpiece material. The present study proposes a new development in the traditionally available cutting tools, which acts like a chip breaker. A series of micro-grooves are machined on the rake face of Tungsten carbide (WC) cutting tools using sinker Electric Discharge Machine (EDM). These micro-grooved tools are used to dissolve long continuous chips and heat from the cutting zone in the dry machining of AISI 1040 steel. The results of micro-grooved cutting tools have shown improvement in reducing the tool tip temperatures and surface roughness compared to the conventional cutting tools. The consolidated chip flow phenomenon of plain WC tools is used to decide the location of micro-grooves on the tool rake face. A Taguchi orthogonal array is used to design an experimental layout with minimum number of repetitions in the experiments. Signal-to-noise  ratios and ANOVA is used to understand and identify the significant factors & their level among the input variables on responses.From the results, it is identified that the cutting speed is the most influencing parameter for tool tip temperature at level 1 (i.e. 112 m/min) and surface roughness at level 5 (i.e. 720 m/min)

Qualitative and quantitative phytochemical screening and in vitro anti oxidant and anti microbial activities of Elephantopus scaber Linn.

Preliminary phytochemical analysis and quantification of total phenols, In-vitro antioxidant and anti microbial activities of the different fractions (hydro alcoholic, hexane, ethyl acetate and methanol) of Elephantopus scaber were carried out against five selected pathogenic bacteria and three fungal species. The plant fraction possesses steroids, triterpinoids, saponins, flavonoides, carbohydrates, glycosides and oils. For total phenolic content gallic acid was taken as a standard, the ethyl acetate fraction contains rich phenolic content than other fractions and the methanol fraction shows more DPPH, superoxide and hydroxyl radical scavenging activity. In Anti-microbial activity study all fractions showed good inhibition zone against three organisms i.e., Escherichia coli, Staphylococcus aureus Klebsiella pneumonia among the other test organisms along with Candida spp (fungal organism)

Seroprevalence of dengue virus in a tertiary care hospital, Andhra Pradesh, South India

Background: Dengue fever and Dengue Haemorrhagic fever (DF/DHF) is an acute viral disease caused by Dengue virus. The infection is transmitted by the bite of an infected female mosquito- Aedes aegypti. The Dengue virus causes significant morbidity and mortality in many parts of the world, including India, where it was first isolated in Calcutta, West Bengal during 1945. This study was conducted to know the seroprevalence of Dengue virus in a tertiary care hospital, HyderabadMethods: Blood for serological studies are carefully collected taking due universal precautions from suspected DF/DHF cases (a) as soon as possible after hospital admission or attendance. All the patients were screened for anti-Dengue IgG and IgM antibodies By Enzyme Immunoassay. The study period was 18 months from 2012. Results: From a total of 1327serum samples tested were screened for Dengue IgM and IgG among which 706(53.2%) were positive. 125(17.7%) were only IgM positive and 198(28.05%) of the tested samples showed only IgG positive. 383(54.25%) of the tested samples revealed positive for both IgM and IgG antibodies. Conclusion: Surveillance is prerequisite for monitoring the dengue situation in the area and should be carried out regularly for early detection of an impending outbreak and to initiate timely preventive and control measures