3 research outputs found
Preval锚ncia de pediculose em crian莽as de escola rural em Yucatan, Mexico
We conducted an analytical cross-sectional survey to estimate the prevalence of and factors associated with active head lice infestation. In total 140 children, aged 6 to 16-years, from a public school in rural Yucatan, Mexico, were examined by wet-combing. A structured questionnaire was used to collect information on individuals and the conditions in the surrounding environment. Head lice infestation was found in 19 out of the 140 children tested (13.6%) and this was associated with both lower income (OR 9.9, 95% CI 2.15-45.79, p = 0.003) and a higher frequency of hair washing (OR 8, 95% CI 1.58-50, p = 0.012). Intersectoral control programs that take into account the socioeconomic differences of children should be implemented.Foi conduzido um estudo numa escola publica de 谩rea rural em Yucatan, Mexico com o objetivo de estimar a preval锚ncia de pediculose e fatores de risco associados. Das 140 crian莽as inclu铆das 19 (13.6%) apresentaram pediculose. A infesta莽茫o de piolho foi associada com baixa renda (OR 9.9, 95% CI 2.15-45.79, p = 0.003) e maior frequ锚ncia de lavagem de cabelos (OR 8, 95% CI 1.58-50, p = 0.012). Programas intersetoriais de controle da pediculose que levam em conta as caracter铆sticas socioecon么micas das crian莽as deveriam ser executados
Expresi贸n y activaci贸n de receptores intracelulares TLR7, TLR8 y TLR9 en monocitos de sangre perif茅rica de pacientes infectados con VIH.
Introduction. TLR麓s play a role in host defense in HIV infection
recognizing the viral DNA or RNA. Their activation induces a signaling
pathway that includes the proteins MyD88, IRAK4, TRAF6 and the
transcription factor NF-kBp65. Objective. To determine the expression
of TLR7, TLR8 and TLR9, and activation of its signaling pathway in
monocytes from patients infected with HIV. Methods. Expression of TLR7,
TLR8 and TLR9 was determined in monocytes from HIV-infected patients (n
= 13) and control subjects (n = 13), which were activated with specific
ligands. The expression of MyD88 and NF-kBp65 were determined by flow
cytometry; IRAK4 and TRAF6 were studied by immunoblotting. Results. No
statistical difference was found in the expression of TLR7, 8 and 9 in
monocytes from patients compared to controls, but we observed the
non-significant increased expression of TLR9 in patients. The
activation showed no significant difference in the expression of MyD88
and NF-kBp65 in patients when compared to controls, but were decreased
in stimulated cells over non-stimulated cells. IRAK4 and TRAF6 were not
detected. Conclusions. No statistical difference was observed in the
expression of intracellular TLRs, MyD88 and NFkBp65 in monocytes from
patients compared to controls. This was probably due to effective
antiretroviral therapy being received at the time of study entry.
Additional studies are needed (ARTV) under controlled conditions that
include infected patients with and without ARVT, responders and non-
responders, and work with different cell populations.Introducci贸n. En la infecci贸n por VIH los TLR juegan un papel
en la defensa del hu茅sped al reconocer el ADN o ARN viral. Su
activaci贸n induce la v铆a de se帽alizaci贸n que
incluye las prote铆nas MyD88, IRAK4, TRAF6 y el factor de
transcripci贸n NF-kBp65. Objetivo. Determinar la expresi贸n del
TLR7, TLR8 y TLR9, y activaci贸n de su v铆a de
se帽alizaci贸n en monocitos de pacientes infectados con VIH.
M茅todos. Se determin贸 la expresi贸n de TLR7, TLR8 y TLR9
en monocitos de pacientes infectados con VIH (n =13) y sujetos control
(n =13), se activaron con ligandos espec铆ficos y se determin贸
la expresi贸n de MyD88 y NF-kBp65 por citometr铆a de flujo.
IRAK4 y TRAF6 fueron estudiadas por inmunoelectrotransferencia.
Resultados. No se observ贸 diferencia estad铆stica en la
expresi贸n de TLR7, 8 y 9 en los monocitos de pacientes con
respecto a los controles, pero observamos aumento no significante del
TLR9 en los pacientes. La activaci贸n no mostr贸 diferencia
significativa en la expresi贸n de MyD88 y NF-kBp65 en pacientes con
respecto a los controles, pero se encontraron disminuidas en
c茅lulas estimuladas con respecto a las no estimuladas. IRAK4 y
TRAF6 no se detectaron. Conclusiones. No se observ贸 diferencia en
la expresi贸n de los TLR, ni en la expresi贸n de MyD88 y
NFkBp65, en monocitos de pacientes con respecto a los controles
probablemente debido a la terapia antirretroviral recibida al momento
del estudio. Se sugieren estudios con pacientes con y sin TARV,
respondedores y no respondedores, y trabajar con diferentes poblaciones
celulares
Prevalence of pediculosis capitis in children from a rural school in Yucatan, Mexico
We conducted an analytical cross-sectional survey to estimate the prevalence of and factors associated with active head lice infestation. In total 140 children, aged 6 to 16-years, from a public school in rural Yucatan, Mexico, were examined by wet-combing. A structured questionnaire was used to collect information on individuals and the conditions in the surrounding environment. Head lice infestation was found in 19 out of the 140 children tested (13.6%) and this was associated with both lower income (OR 9.9, 95% CI 2.15-45.79, p = 0.003) and a higher frequency of hair washing (OR 8, 95% CI 1.58-50, p = 0.012). Intersectoral control programs that take into account the socioeconomic differences of children should be implemented