335 research outputs found
Two isoforms of vasa maternal factor in Senegalese sole: Biotechnological applications
Primordial Germ Cells (PGCs)
identification and manipulation
present considerable potential
for hatchery practice and
surrogate broodstocks.
To carry out the PGCs
characterization a specific
molecular marker is required.
The vasa gene is a good
candidate to identify PGCs and
others germinal cells (Nagasawa
et al., 2009).
The aim of this study was the
cloning of the Solea senegalensis
vasa cDNA and its expression
pattern during early development
and adulthood
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea
A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.
Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases
Caracterização morfocultural de bactérias isoladas de nódulos de espécies de Crotalåria.
bitstream/item/76432/1/DOC276-10.pd
Conservation Biogeography of the SaharaâSahel: additional protected areas are needed to secure unique biodiversity
Aim Identification of priority conservation areas and evaluation of coverage of
the current protected areas are urgently needed to halt the biodiversity loss.
Identifying regions combining similar environmental traits (climate regions)
and species assemblages (biogroups) is needed for conserving the biodiversity
patterns and processes. We identify climate regions and biogroups and map
species diversity across the Sahara-Sahel, a large geographical area that exhibits
wide environmental heterogeneity and multiple species groups with distinct
biogeographical affinities, and evaluate the coverage level of current network of
protected areas for biodiversity conservation.
Location Sahara-Sahel, Africa.
Methods We use spatially explicit climate data with the principal component
analysis and model-based clustering techniques to identify climate regions.
We use distributions of 1147 terrestrial vertebrates (and of 125 Sahara-Sahel
endemics) and apply distance clustering methods to identify biogroups for
both species groups. We apply reserve selection algorithms targeting 17% of
species distribution, climate regions and biogroups to identify priority areas
and gap analysis to assess their representation within the current protected
areas.
Results Seven climate regions were identified, mostly arranged as latitudinal
belts. Concentrations of high species richness were found in the Sahel, but the
central Sahara gathers most endemic and threatened species. Ten biogroups
(five for endemics) were identified. A wide range of biogroups tend to overlap
in specific climate regions. Identified priority areas are inadequately represented
in protected areas, and six new top conservation areas are needed to achieve
conservation targets.
Main conclusions Biodiversity distribution in Sahara-Sahel is spatially structured and apparently related to environmental variation. Although the majority of priority conservation areas are located outside the areas of intense
human activities, many cross multiple political borders and require internationally coordinated efforts for implementation and management. Optimized
biodiversity conservation solutions at regional scale are needed. Our work
contradicts the general idea that deserts are uniform areas and provide
options for the conservation of endangered species.info:eu-repo/semantics/publishedVersio
Paracoccidioidomicose subaguda progressiva: tratamento de um doente com anfotericina B e nutrição parenteral
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Globalisation from Above? Corporate Social Responsibility, the Workers' Party and the Origins of the World Social Forum
In its assessment of the origins and early development of the World Social Forum this article challenges traditional understandings of the Forum as representing âglobalisation from belowâ. By tracing the intricate relations among elements of business, civil society, and the Workersâ Party in the first years of the Forum, this article reveals the major role played by a corporate movement stemming from the Brazilian democratisation process in the 1980s, and how this combined with the transformed agenda of the Workersâ Party as it gained higher political offices to constrain the Forumâs activities from the outset. In so doing, this article challenges not only widespread conceptions of the Forum as a counterâhegemonic alternative but also current critiques concerning its subsequent limitations. Furthermore, it reveals how traditional understandings of the World Social Forum and of global civil society are underpinned by flawed assumptions which typecast political activities in the global âSouthâ
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