Variation Morphologique Adaptative Des Populations De Sarotherodon Melanotheron Des Secteurs IV Et V De La Lagune Ebrié (Côte d’Ivoire) Face Au Stress Chimique

The impact of chemical pollution on organism’s characteristics was assessed through morphological features in IV and V sectors of Ebrié Lagoon using Sarotherodon melanotheron (Rüppel, 1852) as bioindicator. Sampling was carried out during a whole year in three sites (Layo, N’djem and Ahua) characterized by contrasted environments(respectively moderate, medium and high levels of pollution). The morphological studies were based on morphometric biomarkers such as fluctuating asymmetry (FA) and average body shape. Fluctuating asymmetry was evaluated among individuals within sites using ANOVA Procrustes. Average body shape was compared among sampling sites using Principal Components Analysis (PCA), Canonical Variates Analysis (CVA) and MANOVA. Fluctuating asymmetry did not vary between sampling sites. However, the average body shape was significantly different between specimens from Ahua and both those from Layo and N’djem. There was no significant difference between fish from Layo and those from N’djem.Allometric effects and the abiotic parameters influenced, to some extent, the body shape of individuals.A morphological differentiation according to the geographical distance was also observed. The study revealed a morphological adaptation of the populations of S. melanotheron in the study area

Confirmation De Ségrégation, Au Moyen Du BC1, De L’un Des Deux Loci Codant Pour La Couleur Du Germe Chez Le Cocotier Nain à Abidjan (Côte d’Ivoire)

Pour confirmer la ségrégation de l’un des deux loci codant pour la couleur du germe chez les cocotiers nains, le Back cross 1 : NJM x (NJM x GOA+) a été réalisé dans le cadre d’un projet de cartographie du génome du cocotier. Un donneur NJM x GOA+ (Nain jaune de Malaisie x Grand Ouest Africain+) et une receveuse NJM (Nain jaune de Malaisie) ont été impliqués dans le BC1. Les statistiques descriptives, le test U de Mann-Withney et le test du Khi-deux de conformité ont été appliqués. Pour un total de 1034 noix semences germées, les résultats ont montré que sur 5 ségrégations testées, seule la 1:1 vérifie la conformité des données observées à celles théoriques au seuil de risque de 5 %. Ceci suggère que le couple d’allèles Vert/Jaune codant pour l’expression phénotypique y afférente ségrége selon les lois de la génétique classique. Ces résultats s’apparentent à ceux de Bourdeix. Fort malheureusement, son analyse génétique était sous-tendue par de nombreuses hypothèses. De plus, les semences de noix étaient issues de pollinisations libres de pieds d’hybrides F1. Dans les deux cas, ce sont les germes issus des noix F2 germées qui ont été analysées. La ségrégation 1 : 1 issu du BC1 : NJM x (NJM x GOA+) suggère un contrôle monofactoriel du marqueur vert/jaune. Ce marqueur pourrait être introgressé dans les génotypes d’intérêt avant d’être utilisé, par la suite, pour la sélection précoce en germoir.  To confirm the segregation of one of the two loci coding for the color of the germ in dwarf coconut palms, back cross 1: MYD x (MYD x WAT+) was carried out as part of a coconut genome mapping project. An MYD x WAT+ (Malayan Yellow Dwarf x West African Tall+) donor and an MYD (Malayan Yellow Dwarf) recipient were involved in BC1. Descriptive statistics, the Mann-Withney U test and the Chi-two conformance test were applied. For a total of 1034 seed nuts germinated, the results showed that out of 5 segregations tested, only the 1: 1 verifies the compliance of the observed data with the theoretical ones at the risk threshold of 5%. This suggests that the pair of Green / Yellow alleles encoding the related phenotypic expression segregates according to the laws of classical genetics. Those results are similar to those of Bourdeix. Unfortunately, his genetic analysis was underpinned by many hypotheses. In addition, the nut seeds were obtained from free pollination of F1 hybrid feet. In both cases, only germs from the germinated F2 nuts were analyzed. The 1:1 segregation from BC1: MYD x (MYD x WAT+) suggests a monofactorial control of the green / yellow marker. This marker could be moved into the interest of the genotypes before being used, thereafter, for early selection in the germinator.  &nbsp

Influence of Reproductive Rhythm and Weaning Age on Fertility and Body Condition of Local Breed Does in the District of Abidjan

The objective of this study is to evaluate the reproductive performance of locally bred rabbits by comparing the production of females mated 11 days postpartum (semi-intensive R42) with those mated 25 days postpartum (extensive rhythm R56). Females are naturally protruding.120 rabbits selected from a private farm in Bingerville in the district of Abidjan were followed during the experiment. Receptivity and gestation rates were not significantly influenced (p>0.05) by the reproductive rhythm in the breeding females. Fertility in multiparous females showed a higher rate in the extensive rhythm (89-100%). The semi-intensive rhythm had the highest stillbirth rate (5.6%) and pre-weaning morbidity (14.03%). However, after weaning, morbidity was higher in bunnies in the extensive rhythm (13.6%). In the extensive rhythm, the highest values were observed for the number of weaned bunnies and the survival rate of breeding females. The extensive rhythm significantly increases the longevity of does with a high mortality rate of bunny rabbits. These results could be indicators for further investigation in the search for an optimum rate of rabbit reproduction

Antimicrobial Resistance of Klebsiella pneumoniae -ESBL Producing Strains Isolated from Clinical Specimens in Abidjan (Cote de Ivoire)

Aims: The objective of this study is to detect the prevalence of qnr genes in broad-spectrum beta-lactamase producing Klebsiella pneumoniae, involved in hospital and community-acquired infections. Study Design: It is a retrospective study. Place and Duration of Study: Bacteriology and virology laboratorie of Institut Pasteur, Abidjan, Côte d'Ivoire. Methodology: From January 2011 to June 2016, 350 Klebsiella pneumoniae were isolated from various clinical specimens and identified by conventional bacteriological tests. Antibiotic resistance (beta-lactams, quinolones and aminoglycosides) and detection of broad-spectrum beta-lactamases were assessed by the diffusion method and the synergy test, respectively. Molecular characterization of quinolone resistance genes (qnr A, qnr B and qnr S) was performed by the conventional polymerase chain reaction (PCR). Results: Of the 350 Klebsiella pneumoniae isolates, 91(26%) were detected as ESBL producer, 36.2% (n=33) recovered from urine, 24.2% (n=22) from pus and 20.8% (n=19) from blood culture respectively. 46(33%) strains were idenifed to carry qnr genes, qnrB predominate 33(71%), followed by qnr 12(26.1%) and qnrA 1(2.2%). The strains exhibited high resistance to most of the agents tested, expect imipemen, low resistance to amikacin 4.1%, moderate to cefoxitin 31.8% and 54.9% with amoxicillin-clauvanic acid. Conclusion: Although these observed prevalences are small proportions, this can be seen as a warning signal for the future. The emergence and dissemination of resistance genes in Côte d'Ivoire could pose a public health problem. Thus, the establishment of a relevant resistance surveillance policy to better control the circulation of multidrug-resistant strains is necessary

Dwarf short-horned bulls in West Africa: Lagunaire and Muturu breeds

La présente étude est une synthèse des connaissances sur les taurins nains à courtes cornes, notamment les races Lagunaire ou Muturu en Afrique de l’Ouest en général, et en Côte d’Ivoire en particulier. Cette race trypanotolérante se rencontre le long des côtes Ouest Africaines du Libéria au Nigeria, zones infestées de glossines. Le poids moyen des taurins nains à l’âge adulte est de 200 kg pour les mâles contre 160 kg pour les femelles. La hauteur au garrot est comprise entre 80 à 100 cm. L’âge au premier vêlage est de 29,8 mois avec un intervalle inter-vêlages de 13,6 mois. La production laitière de la race Lagunaire est médiocre, soit 0,36 litre par jour. Ces animaux sont généralement élevés pour la boucherie. Les taurins nains à courtes cornes sont remarquablement adaptés à leurs milieux d’élevage où prolifèrent les maladies parasitaires. La race Lagunaire est considérée comme plus tolérante à la trypanosomose que la race Ndama, car elle présente de meilleurs indicateurs. Elle est généralement élevée de façon traditionnelle selon le mode extensif. Les perspectives de développement et de valorisation de cette race sont nombreuses, bien qu’elle n’ait pas encore été prise en compte dans les programmes d’amélioration et de sélection. Mots clés: Taurins nains, Lagunaire, Muturu, trypanosomose, valorisationThis study reviews the knowledge on short-horned dwarf taurines, notably the Lagunaire or Muturu breeds in West Africa in general and in Côte d'Ivoire in particular. This trypanotolerant breed is found along the West African coasts from Liberia to Nigeria, a tsetse-infested areas. The average weight dwarf taurines adults is 200 kg for males and 160 kg for females. The height at the withers is between 80 and 100 cm. The age at first calving is 29.8 months with an inter-calving interval of 13.6 months. The milk production of the Lagunaire breed is low, at 0.36 liters per day. These animals are generally bred for slaughter. The short-horned dwarf bulls are remarkably well adapted to their breeding environments where parasitic diseases proliferate. The Lagunaire breed is considered more trypanosomiasis tolerant than the Ndama breed, as it has better indicators. It is generally reared in the traditional extensive way. The prospects for the development and valorisation of this breed are numerous, although it has not yet been taken into account in the improvement and selection programmes. Keyswords: Dwarf bulls, Laguna, Muturu, trypanosomiasis, valorisatio

Artemisinin derivative-containing therapies and abnormal hemoglobin: Do we need to adapt the treatment?

International audienceBackground: Artemisinin-based treatment in malaria patients with abnormal hemoglobin may be ineffective because of their genetic particularity, which could lead to resistance. The main purpose of this study was to assess the effect of artemisinin derivatives on in vivo parasite clearance according to erythrocyte variants. In vivo response was investigated through retrospective data obtained over a 42-day artemether-lumefantrine/artesunate amodiaquine efficacy protocol conducted from 2012 to 2016. Results: A total of 770 patients in Côte d’Ivoire attending the hospitals of Anonkoua-koute (Abidjan), Petit Paris (Korhogo), Libreville (Man), Dar es salam (Bouaké), Ayamé and Yamoussoukro with acute uncomplicated falciparum malaria were selected for successful hemoglobin typing. HbAS, HbSS, HbAC, and HbSC genotypes were found. Parasite clearance time was obtained for 414 patients. In the population with abnormal hemoglobin, parasite densities on admission and parasite clearance rates were significantly lower in the HbSC group compared to HbAA (p = 0.02 and p = 0.007, respectively). After PCR correction on day 42, the acute treatment rate was 100% for each group. Parasite half-life and time for initial parasitaemia to decline by 50 and 99% were longer for the HbSC group (p < 0.05). The study also investigated the prevalence of K13-propeller polymorphisms across different hemoglobin genotype groups. A total of 185 and 63 samples were sequenced in the HbAA group and patients with abnormal Hb, respectively. Only two nonsynonymous mutations D559N and V510M were found in the HbAA group. Conclusion: Although this study proved good efficacy of artemether-lumefantrine and artesunate amodiaquine in the treatment of uncomplicated Plasmodium falciparum malaria in patients with abnormal hemoglobin, the increased delay of parasite clearance may represent a threat to health in these patients in relation with sickle cell crisis, which could support selection of parasites resistant to artemisinin