Association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells: a comparison between single and combined treatments

The similarity degree of chemical, ionizing radiation, and combined treatments of chemical plus ionizing radiation in their capacity to induce reciprocal translocations was analyzed by means of multivariate analysis techniques on mice germ cells. The effect of three different doses of gamma rays, four doses of X-rays, and different doses of adriamycin, mitomycin C, thio-tepa and bleomycin, as well as the combined treatments of two doses of gamma rays with adriamycin, mitomycin c and thio-tepa, were studied. Our objectives were: 1) to determine the degree of similarity between the effects of chemicals and ionizing radiations in relation to the induction of reciprocal translocations in germ cells; and 2) to test the conclusions reached by previous authors using only single treatments. Data were arranged in a basic data matrix, analyzed by cluster analysis and ordination methods. The results showed that: 1) as single and combined treatments were grouped together, there was not a specifc pattern of chromosomal aberration induced for physical and chemical agents; 2) the association degree between single treatments was similar to that obtained by previous authors, although in this analysis combined treatments were added; and 3) some combined treatments using 9 Gy and drugs appear in different groups, although we expected that all the combined treatments of drugs with 9 Gy were grouped together. As a working hypothesis, we propose that the variability observed when the different treatments were compared could be dose dependent.Por medio de técnicas de análisis multivariado se determinó el grado de similitud de distintos agentes químicos y radiaciones ionizantes en la inducción de translocaciones recíprocas en células germinales de ratón. Se comparó el efecto de tres diferentes dosis de rayos gamma, cuatro dosis de rayos X y distintas dosis de adriamicina, mitomicina C, thio-tepa y bleomicina, así como también los tratamientos combinados con dos dosis de rayos gamma con adriamicina, mitomicina C y tio-tepa, y cuatro de rayos X. Los objetivos del presente trabajo fueron: 1) determinar el grado de similitud entre los tipos de translocaciones recíprocas inducidas por los agentes químicos y las radiaciones ionizantes; y 2) comparar estos resultados con los obtenidos previamente por otros autores al comparar los tratamientos individuales. Para la comparación de los diferentes tratamientos se elaboró una matriz de datos analizada por medio de técnicas de agrupamiento y de ordenación. Los resultados revelaron que: 1) los tratamientos simples y combinados se agruparon juntos, indicando la falta de un patrón específico de aberraciones inducido; 2) el grado de asociación entre los tratamientos simples no se vio modificado, a pesar de la incorporación de los tratamientos combinados; y 3) algunos tratamientos combinados con 9 Gy y las diferentes drogas se asociaron con otros tratamientos, en lugar de asociarse juntos como era de esperarse teniendo en cuenta los resultados obtenidos por otros autores. Como hipótesis de trabajo, proponemos que la variabilidad observada en los diferentes tratamientos podría ser dependiente de la dosis empleada.Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ortiz Jaureguizar, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; ArgentinaFil: Posadas, Paula Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; Argentin

Design of Modular Star Tracker Software

The current CubeSat attitude sensor landscape is bimodal. One end of the spectrum is occupied by solar angle sensors, magnetometers, and rate gyros. However, these low-cost sensors offer insufficient performance for missions that need higher pointing accuracy. Star trackers occupy the other end of the spectrum, providing much greater pointing knowledge accuracy. Unfortunately, the commercially available star trackers are prohibitively expensive for most modest-budget CubeSat missions. To address this problem, the Cal Poly CubeSat Lab (CPCL) decided to implement a star tracker targeted at filling the gap between commercial options and coarse sensors

How does organizational space help organizations cope with the challenges of ambidexterity and continue to innovate? A space reorganization experiment in a transitioning organization

Influenced by internal and external factors, organizations are increasingly operating in divergent fields that require them to develop ambidextrous competencies. While research relating ambidexterity to aspects such as strategy and innovation has reached a maturity stage, we still know little about the strategic processes that allow organizations to implement ambidexterity, and in particular about the role that organizational space can play in an organization\u2019s attempt to become ambidextrous. By conducting a qualitative study in the medical and scientific division of the Italian National Olympic Committee trying to incorporate an exploitation logic in addition to its dominant exploration logic, we describe a two-phase experiment in which the organization leveraged the organizational space as a transition tool towards ambidexterity, while also trying to maintain its exploration innovation-driven competitive advantage. We find that organizational space can be used as a coping tool against identity and competence threats triggered by organizational transition to ambidexterity, affording both integration and differentiation of the ambidextrous logics

Towards Understanding Political Interactions on Instagram

Online Social Networks (OSNs) allow personalities and companies to communicate directly with the public, bypassing filters of traditional medias. As people rely on OSNs to stay up-to-date, the political debate has moved online too. We witness the sudden explosion of harsh political debates and the dissemination of rumours in OSNs. Identifying such behaviour requires a deep understanding on how people interact via OSNs during political debates. We present a preliminary study of interactions in a popular OSN, namely Instagram. We take Italy as a case study in the period before the 2019 European Elections. We observe the activity of top Italian Instagram profiles in different categories: politics, music, sport and show. We record their posts for more than two months, tracking "likes" and comments from users. Results suggest that profiles of politicians attract markedly different interactions than other categories. People tend to comment more, with longer comments, debating for longer time, with a large number of replies, most of which are not explicitly solicited. Moreover, comments tend to come from a small group of very active users. Finally, we witness substantial differences when comparing profiles of different parties.Comment: 5 pages, 8 figure

A deep dive into the pathology of gray platelet syndrome: new insights on immune dysregulation

The gray platelet syndrome (GPS) is a rare platelet disorder, characterized by impaired alpha-granule biogenesis in megakaryocytes and platelets due to NBEAL2 muta-tions. Typical clinical features include macrothrombocytopenia, bleeding and elevated vita-min B12 levels, while bone marrow fibrosis and splenomegaly may develop during disease progression. Recently, the involvement of other blood lineages has been highlighted, reveal-ing the role of NBEAL2 outside the megakaryocyte-platelet axis. Low leukocyte counts, decreased neutrophil granulation and impaired neutrophil extracellular trap formation repre-sent prominent findings in GPS patients, reflecting deranged innate immunity and associated with an increased susceptibility to infection. In addition, low numbers and impaired degra-nulation of NK cells have been demonstrated in animal models. Autoimmune diseases involving different organs and a spectrum of autoantibodies are present in a substantial proportion of GPS patients, expanding the syndromic spectrum of this disorder and pointing to dysregulation of the adaptive immune response. Low-grade inflammation, as evidenced by elevation of liver-derived acute-phase reactants, is another previously unrecognized feature of GPS which may contribute to disease manifestations. This review will focus on the mechanisms underlying the pathogenesis of blood cell abnormalities in human GPS patients and NBEAL2-null animal models, providing insight into the effects of NBEAL2 in hemos-tasis, inflammation and immunity.Fil: Glembotsky, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: de Luca, Geraldine. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Heller, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentin

Black holes in non-local gravity

In this chapter we present a status report of black hole-like solutions in non-local theories of gravity in which the Lagrangians are at least quadratic in curvature and contain specific non-polynomial (i.e., non-local) operators. In the absence of exact black hole solutions valid in the whole spacetime, most of the literature on this topic focus on approximate and simplified equations of motion, which could provide insights on the full non-linear solutions. Therefore, the largest part of this chapter is devoted to the linear approximation. We present results on stationary metric solutions (including both static and rotating cases) and dynamical spacetimes describing the formation of non-rotating mini black holes by the collapse of null shells. Non-local effects can regularize the curvature singularities in both scenarios and, in the dynamical case, there exists a mass gap below which the formation of an apparent horizon can be avoided. In the final part we discuss interesting attempts towards finding non-linear black hole solutions in non-local gravity. Throughout this chapter, instead of focusing on a particular non-local model, we present results valid for large classes of theories (to a feasible extent). This more general approach allows the comparison of similarities and differences of the various types of non-local gravity models.Comment: Invited chapter for the Section "Nonlocal Quantum Gravity" of the "Handbook of Quantum Gravity" (Eds. C. Bambi, L. Modesto and I.L. Shapiro, Springer Singapore, expected in 2023). 29 page

Ventilation Targets for Patients Undergoing Mechanical Thrombectomy for Acute Ischemic Stroke: A Systematic Review

Mechanical thrombectomy (MT) has become a standard treatment for acute ischemic stroke (AIS) caused by large vessel occlusion (LVO). Recent evidence suggests that general anesthesia (GA) and mechanical ventilation do not lead to inferior neurologic outcomes if compared to non-GA. However, the guidelines lack specific recommendations for ventilation targets during MT under GA. This systematic review aims to identify ventilation strategies correlating with better neurological outcomes in AIS patients undergoing MT, particularly focusing on oxygenation and carbon dioxide (CO2) targets. A systematic search of multiple databases was conducted to identify human studies reporting the correlation between ventilation strategies and neurological outcomes in MT for AIS. Eligible studies included clinical trials, observational studies, and case–control studies. Out of 157 studies assessed, 11 met the inclusion criteria. Five studies investigated oxygenation targets, while six studies explored CO2 targets. The published studies highlighted the controversial role of supplemental normobaric oxygen therapy and its potential association with worse outcomes. Regarding CO2 targets, the studies identified a potential association between end tidal CO2 levels and functional outcomes, with hypocapnia being unfavorable. This systematic review demonstrates that the current available evidence still lacks strength to suggest specific ventilation targets, but it highlights the potential risks of hyperoxia and hypocapnia in this specific cohort of patients

Association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells : A comparison between single and combined treatments

Por medio de técnicas de análisis multivariado se determinó el grado de similitud de distintos agentes químicos y radiaciones ionizantes en la inducción de translocaciones recíprocas en células germinales de ratón. Se comparó el efecto de tres diferentes dosis de rayos gamma, cuatro dosis de rayos X y distintas dosis de adriamicina, mitomicina C, thio-tepa y bleomicina, así como también los tratamientos combinados con dos dosis de rayos gamma con adriamicina, mitomicina C y tio-tepa, y cuatro de rayos X. Los objetivos del presente trabajo fueron: 1) determinar el grado de similitud entre los tipos de translocaciones recíprocas inducidas por los agentes químicos y las radiaciones ionizantes; y 2) comparar estos resultados con los obtenidos previamente por otros autores al comparar los tratamientos individuales. Para la comparación de los diferentes tratamientos se elaboró una matriz de datos analizada por medio de técnicas de agrupamiento y de ordenación. Los resultados revelaron que: 1) los tratamientos simples y combinados se agruparon juntos, indicando la falta de un patrón específico de aberraciones inducido; 2) el grado de asociación entre los tratamientos simples no se vio modificado, a pesar de la incorporación de los tratamientos combinados; y 3) algunos tratamientos combinados con 9 Gy y las diferentes drogas se asociaron con otros tratamientos, en lugar de asociarse juntos como era de esperarse teniendo en cuenta los resultados obtenidos por otros autores. Como hipótesis de trabajo, proponemos que la variabilidad observada en los diferentes tratamientos podría ser dependiente de la dosis empleada.The similarity degree of chemical, ionizing radiation, and combined treatments of chemical plus ionizing radiation in their capacity to induce reciprocal translocations was analyzed by means of multivariate analysis techniques on mice germ cells. The effect of three different doses of gamma rays, four doses of X-rays, and different doses of adriamycin, mitomycin C, thio-tepa and bleomycin, as well as the combined treatments of two doses of gamma rays with adriamycin, mitomycin c and thio-tepa, were studied. Our objectives were: 1) to determine the degree of similarity between the effects of chemicals and ionizing radiations in relation to the induction of reciprocal translocations in germ cells; and 2) to test the conclusions reached by previous authors using only single treatments. Data were arranged in a basic data matrix, analyzed by cluster analysis and ordination methods. The results showed that: 1) as single and combined treatments were grouped together, there was not a specifc pattern of chromosomal aberration induced for physical and chemical agents; 2) the association degree between single treatments was similar to that obtained by previous authors, although in this analysis combined treatments were added; and 3) some combined treatments using 9 Gy and drugs appear in different groups, although we expected that all the combined treatments of drugs with 9 Gy were grouped together. As a working hypothesis, we propose that the variability observed when the different treatments were compared could be dose dependent.Instituto de Genética VeterinariaLaboratorio de Sistemática y Biología Evolutiv

Association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells : A comparison between single and combined treatments

Por medio de técnicas de análisis multivariado se determinó el grado de similitud de distintos agentes químicos y radiaciones ionizantes en la inducción de translocaciones recíprocas en células germinales de ratón. Se comparó el efecto de tres diferentes dosis de rayos gamma, cuatro dosis de rayos X y distintas dosis de adriamicina, mitomicina C, thio-tepa y bleomicina, así como también los tratamientos combinados con dos dosis de rayos gamma con adriamicina, mitomicina C y tio-tepa, y cuatro de rayos X. Los objetivos del presente trabajo fueron: 1) determinar el grado de similitud entre los tipos de translocaciones recíprocas inducidas por los agentes químicos y las radiaciones ionizantes; y 2) comparar estos resultados con los obtenidos previamente por otros autores al comparar los tratamientos individuales. Para la comparación de los diferentes tratamientos se elaboró una matriz de datos analizada por medio de técnicas de agrupamiento y de ordenación. Los resultados revelaron que: 1) los tratamientos simples y combinados se agruparon juntos, indicando la falta de un patrón específico de aberraciones inducido; 2) el grado de asociación entre los tratamientos simples no se vio modificado, a pesar de la incorporación de los tratamientos combinados; y 3) algunos tratamientos combinados con 9 Gy y las diferentes drogas se asociaron con otros tratamientos, en lugar de asociarse juntos como era de esperarse teniendo en cuenta los resultados obtenidos por otros autores. Como hipótesis de trabajo, proponemos que la variabilidad observada en los diferentes tratamientos podría ser dependiente de la dosis empleada.The similarity degree of chemical, ionizing radiation, and combined treatments of chemical plus ionizing radiation in their capacity to induce reciprocal translocations was analyzed by means of multivariate analysis techniques on mice germ cells. The effect of three different doses of gamma rays, four doses of X-rays, and different doses of adriamycin, mitomycin C, thio-tepa and bleomycin, as well as the combined treatments of two doses of gamma rays with adriamycin, mitomycin c and thio-tepa, were studied. Our objectives were: 1) to determine the degree of similarity between the effects of chemicals and ionizing radiations in relation to the induction of reciprocal translocations in germ cells; and 2) to test the conclusions reached by previous authors using only single treatments. Data were arranged in a basic data matrix, analyzed by cluster analysis and ordination methods. The results showed that: 1) as single and combined treatments were grouped together, there was not a specifc pattern of chromosomal aberration induced for physical and chemical agents; 2) the association degree between single treatments was similar to that obtained by previous authors, although in this analysis combined treatments were added; and 3) some combined treatments using 9 Gy and drugs appear in different groups, although we expected that all the combined treatments of drugs with 9 Gy were grouped together. As a working hypothesis, we propose that the variability observed when the different treatments were compared could be dose dependent.Instituto de Genética VeterinariaLaboratorio de Sistemática y Biología Evolutiv