697 research outputs found
Ultrasound increases the aqueous extraction of phenolic compounds with high antioxidant activity from olive pomace
Olive pomace is a waste produced by the olive oil industry in massive quantities each year. Disposal of olive pomace is difficult due to high concentrations of phenolic compounds, which is an environmental concern. However, phenolic compounds have applications in the health industry. Therefore, extraction of phenolic compounds from olive pomace has the potential to remove an environmentally hazardous portion of pomace while creating an additional source of income for farmers and producers. Using advanced technologies including Ultrasound Assisted Extraction (UAE), combined with water as an extraction solvent, has recently gained popularity. The present study outlines the optimal UAE conditions for the extraction of phenolic compounds with high antioxidant activity from olive pomace. Optimal conditions were developed using RSM for parameters power, time and sample-to-solvent ratio. Total phenolic compounds determined by Folin Ciocalteu method and total major bioactive compounds determined by HPLC as well as antioxidant capacity (DPPH and CUPRAC) were investigated. The optimal conditions for the extraction of phenolic compounds with high antioxidant activity were 2 g of dried pomace/100 mL of water at 250 W power for 75 min. UAE improved the extraction efficiency of water and yielded extracts with high levels of phenolic compounds and strong antioxidant activity
SWAS and Arecibo observations of H2O and OH in a diffuse cloud along the line-of-sight to W51
Observations of W51 with the Submillimeter Wave Astronomy Satellite (SWAS)
have yielded the first detection of water vapor in a diffuse molecular cloud.
The water vapor lies in a foreground cloud that gives rise to an absorption
feature at an LSR velocity of 6 km/s. The inferred H2O column density is
2.5E+13 cm-2. Observations with the Arecibo radio telescope of hydroxyl
molecules at ten positions in W51 imply an OH column density of 8E+13 cm-2 in
the same diffuse cloud. The observed H2O/OH ratio of ~ 0.3 is significantly
larger than an upper limit derived previously from ultraviolet observations of
the similar diffuse molecular cloud lying in front of HD 154368. The observed
variation in H2O/OH likely points to the presence in one or both of these
clouds of a warm (T > 400) gas component in which neutral-neutral reactions are
important sources of OH and/or H2O.Comment: 15 pages (AASTeX) including 4 (eps) figures. To appear in the
Astrophysical Journa
The olive biophenols oleuropein and hydroxytyrosol selectively reduce proliferation, influence the cell cycle, and induce apoptosis in pancreatic cancer cells
Current chemotherapy drugs for pancreatic cancer only offer an increase in survival of up to six months. Additionally, they are highly toxic to normal tissues, drastically affecting the quality of life of patients. Therefore, the search for novel agents, which induce apoptosis in cancer cells while displaying limited toxicity towards normal cells, is paramount. The olive biophenols, oleuropein, hydroxytyrosol and tyrosol, have displayed cytotoxicity towards cancer cells without affecting non-tumorigenic cells in cancers of the breast and prostate. However, their activity in pancreatic cancer has not been investigated. Therefore, the aim of this study was to determine the anti-pancreatic cancer potential of oleuropein, hydroxytyrosol and tyrosol. Pancreatic cancer cells (MIA PaCa-2, BxPC-3, and CFPAC-1) and non-tumorigenic pancreas cells (HPDE) were treated with oleuropein, hydroxytyrosol and tyrosol to determine their effect on cell viability. Oleuropein displayed selective toxicity towards MIA PaCa-2 cells and hydroxytyrosol towards MIA PaCa-2 and HPDE cells. Subsequent analysis of Bcl-2 family proteins and caspase 3/7 activation determined that oleuropein and hydroxytyrosol induced apoptosis in MIA PaCa-2 cells, while oleuropein displayed a protective effect on HPDE cells. Gene expression analysis revealed putative mechanisms of action, which suggested that c-Jun and c-Fos are involved in oleuropein and hydroxytyrosol induced apoptosis of MIA PaCa-2 cells
Proper Motion of Water Masers Associated with IRAS 21391+5802: Bipolar Outflow and an AU-Scale Dusty Circumstellar Shell
We present VLBA observations of water maser emission associated with the
star-forming region IRAS 21391+5802, which is embedded in a bright rimmed
cometary globule in IC1396. The angular resolution of the maps is about 0.8
mas, corresponding to a spatial resolution of about 0.6 AU, at an estimated
distance of 750 pc. Proper motions are derived for 10 maser features identified
consistently over three epochs, which were separated by intervals of about one
month. The masers appear in four groups, which are aligned linearly on the sky,
roughly along a northeast-southwest direction, with a total separation of about
520 AU (about 0.7 arcseconds). The 3-D velocities of the masers have a maximum
value of about 42 km/s (about 9 AU/yr). The average error on the derived proper
motions is about 4 km/s. The overall pattern of proper motions is indicative of
a bipolar outflow. Proper motions of the masers in a central cluster, with a
projected extent of about 20 AU, show systematic deviations from a radial
outflow. However, we find no evidence of Keplerian rotation, as has been
claimed elsewhere. A nearly circular loop of masers lies near the middle of the
cluster. The radius of this loop is 1 AU and the line-of-sight velocities of
the masers in the loop are within 2 km/s of the systemic velocity of the
region. These masers presumably exist at the radial distance where significant
dust condensation occurs in the outflow emanating from the star.Comment: 15 pages, 4 figures. Accepted for publication in the Astrophysical
Journal. Version 2.12.00: Astrometric coordinates of maser revise
Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype
Abstract Background Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefore sought to determine whether MSCs are present in the lungs of ovalbumin (OVA)-sensitized and challenged mice, a model of allergic airways disease. Methods Balb/c mice were sensitized and challenged with PBS or OVA over a 25 day period. Flow cytometry as well as colony forming and differentiation potential were used to analyze the emergence of MSCs along with gene expression studies using immunochemical analyses, quantitative polymerase chain reaction (qPCR), and gene expression beadchips. Results A CD45-negative subset of cells expressed Stro-1, Sca-1, CD73 and CD105. Selection for these markers and negative selection against CD45 yielded a population of cells capable of adipogenic, osteogenic and chondrogenic differentiation. Lungs from OVA-treated mice demonstrated a greater average colony forming unit-fibroblast (CFU-F) than control mice. Sorted cells differed from unsorted lung adherent cells, exhibiting a pattern of gene expression nearly identical to bone marrow-derived sorted cells. Finally, cells isolated from the bronchoalveolar lavage of a human asthma patient showed identical patterns of cell surface markers and differentiation potential. Conclusions In summary, allergen sensitization and challenge is accompanied by an increase of MSCs resident in the lungs that may regulate inflammatory and fibrotic responses.http://deepblue.lib.umich.edu/bitstream/2027.42/78265/1/1465-9921-11-127.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/2/1465-9921-11-127.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/3/1465-9921-11-127-S1.DOCPeer Reviewe
The Arecibo Methanol Maser Galactic Plane Survey - II: Statistical and Multi-wavelength Counterpart Analysis
We present an analysis of the properties of the 6.7 GHz methanol maser sample
detected in the Arecibo Methanol Maser Galactic Plane Survey. The distribution
of the masers in the Galaxy, and statistics of their multi-wavelength
counterparts is consistent with the hypothesis of 6.7 GHz maser emission being
associated with massive young stellar objects. Using the detection statistics
of our survey, we estimate the minimum number of methanol masers in the Galaxy
to be 1275. The l-v diagram of the sample shows the tangent point of the
Carina-Sagittarius spiral arm to be around 49.6 degrees, and suggests
occurrence of massive star formation along the extension of the Crux-Scutum
arm. A Gaussian component analysis of the maser spectra shows the mean
line-width to be 0.38 km/s which is more than a factor of two larger than what
has been reported in the literature. We also find no evidence that faint
methanol masers have different properties than those of their bright
counterparts.Comment: Accepted by ApJ; Revised footnote number 3 on page 8 based on private
communicatio
A Search for 6.7 GHz Methanol Masers in M33
We report the negative results from a search for 6.7 GHz methanol masers in
the nearby spiral galaxy M33. We observed 14 GMCs in the central 4 kpc of the
Galaxy, and found 3 sigma upper limits to the flux density of ~9 mJy in
spectral channels having a velocity width of 0.069 km/s. By velocity shifting
and combining the spectra from the positions observed, we obtain an effective
3sigma upper limit on the average emission of ~1mJy in a 0.25 km/s channel.
These limits lie significantly below what we would expect based on our
estimates of the methanol maser luminosity function in the Milky Way. The most
likely explanation for the absence of detectable methanol masers appears to be
the metallicity of M33, which is modestly less than that of the Milky Way
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