Evolution of Parasitism and Mutualism between M13 and E. coli (data, stats, and code associated with manuscript)

These data are of growth curves from two assays associated with the manuscript, "Evolution of mutualism and parasitism between filamentous phage M13 and <i>Escherichia coli.</i>" The file "DataSummary" provides raw growth data, whereas "StatSummary" provides the data analyses. "PlateLayouts" lists the arrangement of cultures during the experiment and assays. "ReadMe" provides additional details for interpreting the data in each file. "paramutsim.r" provides code used for a discrete time model explored in the Supplementary Material

Viruses Evolved under Low Co-Infection Are More Robust than Those Evolved under High Co-Infection

<p>Each point is the mean (<i>n</i> = 3, ± standard error of the mean) change in log₁₀ fitness (Δlog₁₀<i>W</i>) resulting from mutation accumulation, for an independent lineage founded by a virus clone evolved under low level of co-infection (circles) or high level of co-infection (squares). Horizontal lines are grand means for Δlog₁₀<i>W</i> among lineages within a treatment, and the dashed lines indicate one standard deviation away from the grand mean.</p

Differences in Virus Robustness Are Not Confounded by Increased Mutation Frequencies in Viruses Evolved under High Co-Infection

<p>Mean mutation frequency (± 95% confidence interval) was assayed for wild-type ϕ6 (ANC), and for one pre-bottleneck clone isolated from each population evolved under low co-infection (L1–L3) and high co-infection (H1–H3). Assays on <i>P. tomato</i> (circles) and <i>P. atrofaciens</i> (squares) were replicated 5-fold. Assays for <i>P. atrofaciens</i> mutants challenged with growth on <i>P. tomato</i> (diamonds) were replicated 6-fold, except for H3 (<i>n</i> = 2).</p

Figure 5. Selection in temporally heterogeneous innate immune environments leads to VSV strains with differential capacities to block IFN-β production.

This data corresponds to the graph in Figure 5. It describes the secretion of IFN-b in PC3 cells (as measured by ELISA) after infection with different strains of VSV experimentally evolved in heterogeneous innate immune environments