Differences in Virus Robustness Are Not Confounded by Increased Mutation Frequencies in Viruses Evolved under High Co-Infection

Abstract

<p>Mean mutation frequency (± 95% confidence interval) was assayed for wild-type ϕ6 (ANC), and for one pre-bottleneck clone isolated from each population evolved under low co-infection (L1–L3) and high co-infection (H1–H3). Assays on <i>P. tomato</i> (circles) and <i>P. atrofaciens</i> (squares) were replicated 5-fold. Assays for <i>P. atrofaciens</i> mutants challenged with growth on <i>P. tomato</i> (diamonds) were replicated 6-fold, except for H3 (<i>n</i> = 2).</p