Acute effect of repeated sprints on inter-limb asymmetries during unilateral jumping

The aim of the present study was to investigate the effects of multiple repeated sprints on unilateral jump performance and inter-limb asymmetries. Eighteen recreationally active males performed three single leg countermovement jumps (SLCMJ) as baseline data. The repeated sprint protocol was 6 x 40 m with 20 seconds of passive rest between each sprint. This protocol was conducted four times, each set separated by four minutes of rest. Within that rest period, subjects performed one SLCMJ on each limb after two minutes of rest. A one-way ANOVA showed significant reductions (p < 0.05; ES = -0.52 to -0.99) in jump height on both limbs after each set relative to baseline. Inter-limb asymmetries increased at each time point and ranged from 7.62-14.67%, with significant increases in asymmetry seen after sets three (p = 0.046) and four (p = 0.002). Significant increases in sprint time were shown between sprints one and six in each set (p ≤ 0.01). A fatigue index (%) was also calculated and showed an exponential increase from 5.74% (set one) to 13.50% (set four), with significant differences between all sets (p < 0.001) with the exception of sets three and four. Results from this study show that a 6 x 40 m repeated sprint protocol is a sufficient dose for implementing acute fatigue in recreationally active subjects. This was manifested by reductions in jump height at all time points and jump height asymmetries after the third and fourth sets. These findings indicate that jump height from unilateral jump testing may be a useful metric to use during the monitoring process in recreationally trained athletes

Peculiar Velocities of Nonlinear Structure: Voids in McVittie Spacetime

As a study of peculiar velocities of nonlinear structure, we analyze the model of a relativistic thin-shell void in the expanding universe. (1) Adopting McVittie (MV) spacetime as a background universe, we investigate the dynamics of an uncompensated void with negative MV mass. Although the motion itself is quite different from that of a compensated void, as shown by Haines & Harris (1993), the present peculiar velocities are not affected by MV mass. (2) We discuss how precisely the formula in the linear perturbation theory applies to nonlinear relativistic voids, using the results in (1) as well as the previous results for the homogeneous background (Sakai, Maeda, & Sato 1993). (3) We re-examine the effect of the cosmic microwave background radiation. Contrary to the results of Pim & Lake (1986, 1988), we find that the effect is negligible. We show that their results are due to inappropriate initial conditions. Our results (1)-(3) suggest that the formula in the linear perturbation theory is approximately valid even for nonlinear voids.Comment: 12 pages, aastex, 4 ps figures separate, Fig.2 added, to appear in Ap

Wolbachia Endosymbionts Modify Drosophila Ovary Protein Levels in a Context-Dependent Manner

ABSTRACT Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is Wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that contribute to maternal transmission have been identified, the molecular basis for maternal Wolbachia transmission remains largely unknown. To investigate transmission-related processes in response to Wolbachia infection, ovarian proteomes were analyzed from Wolbachia-infected Drosophila melanogaster and D. simulans. Endogenous and variant host-strain combinations were investigated. Significant and differentially abundant ovarian proteins were detected, indicating substantial regulatory changes in response to Wolbachia. Variant Wolbachia strains were associated with a broader impact on the ovary proteome than endogenous Wolbachia strains. The D. melanogaster ovarian environment also exhibited a higher level of diversity of proteomic responses to Wolbachia than D. simulans. Overall, many Wolbachia-responsive ovarian proteins detected in this study were consistent with expectations from the experimental literature. This suggests that context-specific changes in protein abundance contribute to Wolbachia manipulation of transmission-related mechanisms in oogenesis. IMPORTANCE Millions of insect species naturally carry bacterial endosymbionts called Wolbachia. Wolbachia bacteria are transmitted by females to their offspring through a robust egg-loading mechanism. The molecular basis for Wolbachia transmission remains poorly understood at this time, however. This proteomic study identified specific fruit fly ovarian proteins as being upregulated or downregulated in response to Wolbachia infection. The majority of these protein responses correlated specifically with the type of host and Wolbachia strain involved. This work corroborates previously identified factors and mechanisms while also framing the broader context of ovarian manipulation by Wolbachia

Hsp31 Is a Stress Response Chaperone That Intervenes in the Protein Misfolding Process

The Saccharomyces cerevisiae heat shock protein Hsp31 is a stress-inducible homodimeric protein that is involved in diauxicshift reprogramming and has glyoxalase activity. We show thatsubstoichiometric concentrations of Hsp31 can abrogate aggrega-tion of a broad array of substrates in vitro. Hsp31 also modulates the aggregation of -synuclein ( Syn), a target of the chaperoneactivity of human DJ-1, an Hsp31 homolog. We demonstrate thatHsp31 is able to suppress the in vitro fibrillization or aggregation of Syn, citrate synthase and insulin. Chaperone activity was also observed in vivo because constitutive overexpression of Hsp31 reduced the incidence of Syn cytoplasmic foci, and yeast cells were rescued from Syn-generated proteotoxicity upon Hsp31overexpression. Moreover, we showed that Hsp31 protein levels are increased byH2O2, in the diauxic phase of normal growth con-ditions, and in cells under Syn-mediated proteotoxic stress. Weshow that Hsp31 chaperone activity and not the methylglyoxalaseactivity or the autophagy pathway drives the protective effects.Wealso demonstrate reduced aggregation of the Sup35 prion domain,PrD-Sup35, as visualized by fluorescent protein fusions. In addi-tion, Hsp31 acts on its substrates prior to the formation of largeaggregates because Hsp31 does not mutually localize with prionaggregates, and it prevents the formation of detectable in vitro Syn fibrils. These studies establish that the protective role ofHsp31 against cellular stress is achieved by chaperone activity thatintervenes early in the protein misfolding process and is effectiveona wide spectrum of substrate proteins, including Synandprion proteins

Bilateral vs. unilateral countermovement jumps: comparing the magnitude and direction of asymmetry in elite academy soccer players

The aims of the present study were to compare the magnitude and direction of asymmetry in comparable bilateral and unilateral countermovement jumps (CMJ). Forty-five elite academy soccer players from under-23 (n = 15), under-18 (n = 16) and under-16 (n = 14) age groups performed bilateral and unilateral CMJ as part of their routine pre-season fitness testing. For the magnitude of asymmetry, no significant differences were evident for any metric between tests. However, eccentric impulse asymmetry was significantly greater than mean force and concentric impulse in both bilateral and unilateral tests (p < 0.01). For the direction of asymmetry, Kappa coefficients showed poor levels of agreement between test measures for all metrics (mean force = -0.15; concentric impulse = -0.07; eccentric impulse = -0.13). Mean jump data was also presented relative to body mass for each group. For the bilateral CMJ, significant differences were evident between groups, but showed little consistency in the same group performing better or worse across metrics. For the unilateral CMJ, eccentric impulse was the only metric to show meaningful differences between groups, with the under-18 group performing significantly worse than under-23 and under-16 players. This study highlights that despite the magnitude of asymmetry being similar for each metric between comparable bilateral and unilateral CMJ, consistency in the direction of asymmetry was poor. In essence, if the right limb produced the larger force or impulse during a bilateral CMJ, it was rare for the same limb to perform superior during the unilateral task. Thus, practitioners should be aware that bilateral and unilateral CMJ present different limb dominance characteristics and should not use one test to represent the other when measuring between-limb asymmetries

A practical guide to analyzing the force-time curve of isometric tasks in Excel

Understanding force generating capabilities of athletes is an important facet of strength diagnostics. The utilisation of isometric tasks such as the isometric squat and isometric mid-thigh pull are therefore popular methods used to gain a deeper understanding of as to what strength characteristics have changed over a given period. This article aims to provide information on how to understand and analyse the force time curve of isometric tasks in Microsoft Excel, thus providing practitioners an inexpensive and accessible alternative to readily available software on the market

Drop jump asymmetry is associated with reduced sprint and change-of-direction speed performance in adult female soccer players

Studies that examine the effects of inter-limb asymmetry on measures of physical performance are scarce, especially in adult female populations. The aim of the present study was to establish the relationship between inter-limb asymmetry and speed and change-of-direction speed (CODS) in adult female soccer players. Sixteen adult players performed a preseason test battery consisting of unilateral countermovement jump (CMJ), unilateral drop jump (DJ), 10 m, 30 m, and 505 CODS tests. Inter-limb asymmetry was calculated using a standard percentage difference equation for jump and CODS tests, and Pearson's r correlations were used to establish a relationship between asymmetry and physical performance as well as asymmetry scores themselves across tests. Jump-height asymmetry from the CMJ (8.65%) and DJ (9.16%) tests were significantly greater (p < 0.05) than asymmetry during the 505 test (2.39%). CMJ-height asymmetry showed no association with speed or CODS. However, DJ asymmetries were significantly associated with slower 10 m (r = 0.52; p < 0.05), 30 m (r = 0.58; p < 0.05), and 505 (r = 0.52⁻0.66; p < 0.05) performance. No significant relationships were present between asymmetry scores across tests. These findings suggest that the DJ is a useful test for detecting existent between-limb asymmetry that might in turn be detrimental to speed and CODS performance. Furthermore, the lack of relationships present between different asymmetry scores indicates the individual nature of asymmetry and precludes the use of a single test for the assessment of inter-limb differences

Field-induced effects in the spin liquid candidate PbCuTe2_{2}O6_{6}

PbCuTe$_2$O$_6$ is considered as one of the rare candidate materials for a three-dimensional quantum spin liquid (QSL). This assessment was based on the results of various magnetic experiments, performed mainly on polycrystalline material. More recent measurements on single crystals revealed an even more exotic behavior, yielding ferroelectric order below $T_{\text{FE}}\approx 1\,\text{K}$, accompanied by distinct lattice distortions, and a somewhat modified magnetic response which is still consistent with a QSL. Here we report on low-temperature measurements of various thermodynamic, magnetic and dielectric properties of single crystalline PbCuTe$_2$O$_6$ in magnetic fields $B\leq 14.5\,\text{T}$. The combination of these various probes allows us to construct a detailed $B$-$T$ phase diagram including a ferroelectric phase for $B \leq$ $8\,\text{T}$ and a $B$-induced magnetic phase at $B \geq$ $11\,\text{T}$. These phases are preceded by or coincide with a structural transition from a cubic high-temperature phase into a distorted non-cubic low-temperature state. The phase diagram discloses two quantum critical points (QCPs) in the accessible field range, a ferroelectric QCP at $B_{c1}$ = $7.9\,\text{T}$ and a magnetic QCP at $B_{c2}$ = $11\,\text{T}$. Field-induced lattice distortions, observed in the state at $T>$ $1\,\text{K}$ and which are assigned to the effect of spin-orbit interaction of the Cu$^{2+}$-ions, are considered as the key mechanism by which the magnetic field couples to the dielectric degrees of freedom in this material

Force-time characteristics of the countermovement jump: analyzing the curve in Excel

Increased popularity in the utilization of force plates to measure countermovement jumps (cmjs) for performance monitoring warrants the need for strength and conditioning coaches and sport scientists to better under-stand its force-time characteristics and the calculation of its associated variables. this article aims to provide information on how to understand and analyze the force-time curve of cmjs in microsoft excel, thus providing practitioners an inexpensive and accessible alternative to readily avail-able software on the market

Relative strength explains the differences in multi-joint rapid force production between sexes

The primary aim of this study was to determine whether relative strength explains the differences in the rapid force production (force developed during first 150-, 200-, and 250 ms) of females and males, and to evaluate the relationships between peak force and rapid force production. Sixty-three team sport athletes (females: n = 25, age = 21.5 ± 1.3 years, stature = 166 ± 5 cm, body mass = 60.65 ± 10.04 kg; males: n = 38, age = 21.9 ± 1.1 years, stature = 178 ± 7 cm, body mass = 76.55 ± 12.88 kg) performed a series of isometric mid-thigh pull (IMTP) trials, with all participants' data used for correlational analysis. After testing, females and males were divided into 20 strength-matched pairs, based on their relative peak force (peak force ∙ body mass). There were no meaningful differences between sexes for relative force at 150 ms (g = 0.007 [95% CI -0.627, 0.648]), 200 ms (g = -0.059 [95% CI -0.695, 0.588]) and 250 ms (g = -0.156 [95% CI -0.778, 0.473]). Similarly, when expressed as a percentage of peak force there were no meaningful differences in force at 150 ms (g = -0.015 [95.0%CI -0.650, 0.680]), 200 ms (g = -0.099 [95.0%CI -0.714, 0.559]) or 250 ms (g = -0.272 [95.0%CI -0.856, 0.328]) between strength-matched females and males. Based on the correlations, there were very large to nearly perfect relationships (r = 0.77-0.94, p <0.001) between peak force and rapid force production, with peak force explaining 59%, 77% and 89% of the variance in force at 150-, 200- and 250 ms, respectively. When comparing females and males, relative strength (based on body weight or a percentage of peak force) should be considered, and practitioners should be aware of the role of peak force in rapid force production