Production of 4-keto-D-arabonate by oxidative fermentation with newly isolated Gluconacetobacter liquefaciens

Production of 4-keto-D-arabonate (4KAB) was confirmed in a culture medium of Gluconacetobacter liquefaciens strains, newly isolated from water kefir in Argentina. The strains rapidly oxidized D-glucose, Dgluconate (GA), and 2-keto-D-gluconate (2KGA), and accumulated 2,5-diketo-D-gluconate (25DKA) exclusively before reaching the stationary phase. 25DKA was in turn converted to 4KAB, and 4KAB remained stable in the culture medium. The occurrence of 4KAB was assumed by Ameyama and Kondo about 50 years ago in their study on the carbohydrate metabolism of acetic acid bacteria (Bull. Agr. Chem. Soc. Jpn., 22, 271-272, 380-386 (1958)). This is the first report confirming microbial production of 4KAB.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Production of 4-keto-D-arabonate by oxidative fermentation with newly isolated Gluconacetobacter liquefaciens

Production of 4-keto-D-arabonate (4KAB) was confirmed in a culture medium of Gluconacetobacter liquefaciens strains, newly isolated from water kefir in Argentina. The strains rapidly oxidized D-glucose, Dgluconate (GA), and 2-keto-D-gluconate (2KGA), and accumulated 2,5-diketo-D-gluconate (25DKA) exclusively before reaching the stationary phase. 25DKA was in turn converted to 4KAB, and 4KAB remained stable in the culture medium. The occurrence of 4KAB was assumed by Ameyama and Kondo about 50 years ago in their study on the carbohydrate metabolism of acetic acid bacteria (Bull. Agr. Chem. Soc. Jpn., 22, 271-272, 380-386 (1958)). This is the first report confirming microbial production of 4KAB.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Systematic Study of Acetic Acid Bacteria Isolated in Thailand

博士論文 (Doctoral dissertation

Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey

A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale

The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)

<p><strong>Abstract:</strong> The genus Kloeckeraspora Niehaus (1932) was not accepted for a long time. This paper has tried to reinstate the genus. Two phylogenetic trees were constructed for three Kloeckeraspora and four representative Hanseniaspora species. In addition, the pair-wise sequence similarities were calculated. In the phylogeneetic tree based on the 26S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method, the three species of the genus Kloeckeraspora represented an extremely long branch. The calculated pair-wise sequence similarities were abnormally low (86.2 - 88.5%) between the two genera. Incidentally, the sequence similarity between Vanderwatozyma polyspora and Saccharomyces cerevisiae used as references was 94.0%. The genus Kloeckeraspora should be unequivocally accepted and retained. However, the two genera were not yet perfectly taxonomic homogeneous-natured.</p> <p><strong>Keywords:</strong> Kloeckeraspora osmophila; Kloeckerasora vineae; Kloeckeraspora occidentalis; Hanseniaspora valvyensis; Hanseniaspora uvarum; Hanseniaspora guilliermondii.</p> <p><strong>Title:</strong> The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)</p> <p><strong>Author:</strong> Taweesak Malimas, Huong Thi Lan Vu, Pattaraporn Yukphan, Somboon Tanasupawat, Yuzo Yamada</p> <p><strong>International Journal of Novel Research in Life Sciences                                                    </strong></p> <p><strong>ISSN 2394-966X</strong></p> <p><strong>Vol. 10, Issue 2, March 2023 - April 2023</strong></p> <p><strong>Page No: 32-37</strong></p> <p><strong>Novelty Journals</strong></p> <p><strong>Website: www.noveltyjournals.com</strong></p> <p><strong>Published Date: 03-April-2023</strong></p> <p><strong>Amendment Date: 05-October-2023</strong></p> <p><strong>DOI: <a href="https://doi.org/10.5281/zenodo.8409923">https://doi.org/10.5281/zenodo.8409923</a></strong></p> <p><strong>Paper Download Link (Source)</strong></p> <p><a href="https://www.noveltyjournals.com/upload/paper/The%20Reinstatement%20of%20the%20Genus-03042023-1.pdf"><strong>https://www.noveltyjournals.com/upload/paper/The%20Reinstatement%20of%20the%20Genus-03042023-1.pdf</strong></a></p>International Journal of Novel Research in Life Sciences, ISSN 2394-966X, Novelty Journals, Website: www.noveltyjournals.co

Production of 4-keto-D-arabonate by oxidative fermentation with newly isolated Gluconacetobacter liquefaciens

Production of 4-keto-D-arabonate (4KAB) was confirmed in a culture medium of Gluconacetobacter liquefaciens strains, newly isolated from water kefir in Argentina. The strains rapidly oxidized D-glucose, Dgluconate (GA), and 2-keto-D-gluconate (2KGA), and accumulated 2,5-diketo-D-gluconate (25DKA) exclusively before reaching the stationary phase. 25DKA was in turn converted to 4KAB, and 4KAB remained stable in the culture medium. The occurrence of 4KAB was assumed by Ameyama and Kondo about 50 years ago in their study on the carbohydrate metabolism of acetic acid bacteria (Bull. Agr. Chem. Soc. Jpn., 22, 271-272, 380-386 (1958)). This is the first report confirming microbial production of 4KAB.Fil: Adachi, Osao. Yamaguchi University; JapónFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Akakabe, Yoshihiko. Yamaguchi University; JapónFil: Tanasupawat, Somboon. Chulalongkorn University; TailandiaFil: Yukphan, Pattaraporn. No especifíca;Fil: Shinagawa, Emiko. No especifíca;Fil: Yakushi, Toshiharu. Yamaguchi University; JapónFil: Matsushita, Kazunobu. Yamaguchi University; Japó

The generic circumscription of Mrakia and Mrakiella: The proposal of Thomashallia gen. nov.

<p><strong>Abstract:</strong> In the family Mrakiaceae, the type genus <em>Mrakia</em> senseu stricto included five teleomorphic species with the type species,<em> Mrakia frigida</em>. In contrast, the anamorphic genus <em>Mrakiella</em> sensu stricto contained nine species with the type species, <em>Mrakiella cryoconiti</em>. Between the two genera, the completely separated clusters were shown respectively in the phylogenetic tree based on the 28S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method. Between <em>Mrakia frigida</em> and <em>Mrakiella</em> <em>cryoconiti</em> as well as <em>Mrakiella aquatica</em>, the pair-wise sequence similarities were 98.6% and 97.4% (1.2% width) respectively. However, the similarities between <em>Mrakia frigida</em> and the remaining four <em>Mrakia</em> species were extremely high (99.4 - 100% with 0.6% width). On the other hand, the similarity was relatively low (98.2%) between <em>Mrakiella</em> <em>cryoconiti</em> and <em>Mrakiella aquatica</em>, showing the wide range or the diversity of the anamorphic genus phylogenetically. The teleomorphic genus <em>Thomashallia</em> was newly introduced based on the formation of basidia and basidiospores with the type species, <em>Thomashallia stelviica</em>. The genus <em>Krasilnikovozyma</em> contained four species with the type species, <em>Krasilnikovozyma curviuscula</em>. Thus, the three teleomorphic genera were respectively taxonomic homogeneous-natured, and the two of the three were surely characteristic of Q-8.   </p> <p><strong>Keywords:</strong> <em>Mrakia frigida</em>; <em>Mrakiella cryoconiti</em>; <em>Thomashallia </em>gen. nov.; <em>Thomashallia stelviica</em> comb. nov.; <em>Thomashallia montana</em> comb. nov.</p> <p><strong>Title:</strong> The generic circumscription of <em>Mrakia </em>and <em>Mrakiella</em>: The proposal of <em>Thomashallia</em> gen. nov.</p> <p><strong>Author:</strong> Taweesak Malimas, Huong Thi Lan Vu, Pattaraporn Yukphan, Somboon Tanasupawat, Yuzo Yamada</p> <p><strong>International Journal of Novel Research in Life Sciences                                                    </strong></p> <p><strong>ISSN 2394-966X</strong></p> <p><strong>Vol. 10, Issue 5, September 2023 - October 2023</strong></p> <p><strong>Page No: 33-41</strong></p> <p><strong>Novelty Journals</strong></p> <p><strong>Website: www.noveltyjournals.com</strong></p> <p><strong>Published Date: 10-October-2023</strong></p> <p><strong>DOI: <a href="https://doi.org/10.5281/zenodo.8424882">https://doi.org/10.5281/zenodo.8424882</a></strong></p> <p><strong>Paper Download Link (Source)</strong></p> <p><strong><a href="https://www.noveltyjournals.com/upload/paper/The%20generic%20circumscription%20of%20Mrakia-10102023-4.pdf">https://www.noveltyjournals.com/upload/paper/The%20generic%20circumscription%20of%20Mrakia-10102023-4.pdf</a></strong></p>International Journal of Novel Research in Life Sciences, ISSN 2394-966X, Novelty Journals, Website: www.noveltyjournals.co