In-Vitro Anticoagulant Study of Leaf Extract of Couropita Guianensis

Anticoagulant activity of different extract of cannon ball tree was evaluated in-vitro. Medicinal plant acts as a metabolic bio factory, which can be accessed as therapeutic potential with lesser side effects. The phyto constituents of various medicinal plants can be correlated with pharmacological study. In vitro study was carried out with a vision to setup standards that could be beneficial for detecting authenticity of this medicinal plant. Numerical standards reported in this work could be useful for the compilation of suitable monograph of Couropita guianensis. Various phyto constituents such as alkaloids, tannins, saponnins and glycosides are producing a better opportunity for testing various pharmacological activities. The results obtained from this research revealed that that the plant contains bioactive agents which are connected with anticoagulant activity in plants

EXPERIMENTAL EVALUATION OF ANTIDEPRESSANT ACTIVITY OF AQUEOUS AND CHLOROFORM LEAF AND SHOOT EXTRACTS OF EICCHORNIA CRASSIPES LINN IN MICE

Objective: To investigate antidepressant activity of aqueous and chloroform extract of Eicchornia crassipes plant leaves and shoots in mice.Methods: The antidepressant activity of aqueous and chloroform extract of Eicchornia crassipes plant leaves and shoots were tested by forced swimtest (FST) and tail suspension test (TST) in albino mice and the results were compared for the both extracts. Imipramine was used as the standardrug for comparison. Results: Phytochemical screening showed presence of carbohydrates, alkaloids, flavanoids, steroids, saponins, amino acids, gums and mucilage.aqueous extract of Eicchornea crassipes (AEEC) and chloroform extract of E. crassipes (CEEC) did not produce any lethal effect even upto 2000 mg/kp.o during acute oral toxicity study. In FST and TST, CEEC showed diminution of duration of immobility time in 200 mg/kg but not in 100 mg/kg. Conclusion: From the above finding concluding that, shortening of immobility time in the FST and TST indicating, CEEC showed more antidepressantactivity acting either by the enhancement of central 5-HT or catecholamine neurotransmission compared to AEEC in mice.Keywords: Eicchornia crassipes, Aqueous extract of Eicchornia crassipes, Chloroform extract of Eicchornia crassipes, Forced swim test,Tail suspension test

SYNTHESIS & IN-VITRO PROTEIN DENATURATION SCREENING OF 2-[(1, 5-DISUBSTITUTEDPHENYL-4,5-DIHYDRO-1H-PYRAZOL-3-YL)OXY]BENZOIC ACID DERIVATIVES

Abstract Novel 2-[(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)oxy]benzoic acid (2a) & 2-[5-(2-hydroxyphenyl)-1-Phenyl-4,5-dihydro-1H-pyrazol-3-yl]benzoic acid (2b) were produced and examined for their in-vitro protein denaturation activities. It was discovered that compound 2b showed promise and had more potency than acetylsalicylic acid (NSAID) in inhibiting denaturation of bovine serum albumin. Docking research also supports this. The compound 2b has the highest docking scores with COX1(PDB ID 3N8Z), COX2 (PDB ID 4PH9), and TNF (PDB ID 2AZ5), respectively, of Etotal -233.75, -256.48, and -255.83. TLC and elemental tests were used to determine the compounds' purity. All of the generated molecules' analytical and spectral data (1H NMR, FTIR, and MS) were entirely consistent with the proposed structures

Procalcitonin albumin ratio: A useful predictor in intervention of urosepsis from febrile urinary tract infection

Because procalcitonin has been recommended as a bacterial infection marker, this study was conducted to investigate the utility of serum PCT as an early marker to decide on intervention for urinary tract infection.  We did a one-year retrospective analysis with 200 individuals who had febrile urinary tract infections. The independent risk variables for distinguishing urosepsis from febrile urinary tract infection were identified using univariate and multivariate logistic analysis. To examine the prediction accuracy of the procalcitonin/albumin ratio, a receiver operating characteristic (ROC) curve analysis was performed. The procalcitonin/albumin ratios in the urosepsis group were greater than those in the febrile urinary tract infection group were 84.62 percent and 96.00 percent, respectively. Furthermore, in the subset of 65 patients with urosepsis, the procalcitonin/albumin ratio in the uroseptic shock group was higher than in the non-urosepsis group. Our findings show that the procalcitonin/albumin ratio can distinguish between urosepsis and febrile urinary tract infection early in the diagnostic process. Furthermore, patients with urosepsis who had greater procalcitonin/albumin ratios were more likely to develop uroseptic shock. Our findings show that the procalcitonin/albumin ratio can be employed in clinical practise as a quick and low-cost biomarker

Formulation and in-vitro evaluation of Levocetirizine topical gel

ABSTRACT A second-generation histamine antagonist known as Levocetirizinedihydrochloride (LTZ) is used to treat a variety of allergy symptoms as well as skin conditions like hives, including itching, rashes, and dermatitis. When used orally, LTZ produces hepatotoxicity and sleepiness. The development and evaluation of a topical gel that can prevent systemic adverse effects was the goal of this study. As a result, different ratios/grades of carbopol were used as primary gelling agents and varied ratios of cellulose derivatives were used as combination polymers to create the gel. There were a total of thirteen (13) formulations created and evaluated physically as well as for other characteristics like drug content percentage, pH, spreadability, extrudability, homogeneity, consistency, in-vitro drug release, viscosity, and skin irritability. Drug purity and compatibility with excipients were shown by research using Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC).Rejected formulations were those that failed the physical appearance and homogeneity tests. The viscosity and in-vitro drug release research of three formulations that performed best in the homogeneity test were assessed. The formulation F6, which demonstrated the highest percentage of drug release, was then tested for skin irritancy on volunteers before being stabilized for three months under accelerated storage conditions. The F6 formulation shown positive qualities and it is further advised that it be tested for ex vivo permeability and topical absorption

Chemical diversity of dietary phytochemicals and their mode of chemoprevention

Despite the advancement in prognosis, diagnosis and treatment, cancer has emerged as the second leading cause of disease-associated death across the globe. With the remarkable application of synthetic drugs in cancer therapy and the onset of therapy-associated adverse effects, dietary phytochemicals have been materialized as potent anti-cancer drugs owing to their antioxidant, apoptosis and autophagy modulating activities. With dynamic regulation of apoptosis and autophagy in association with cell cycle regulation, inhibition in cellular proliferation, invasion and migration, dietary phytochemicals have emerged as potent anti-cancer pharmacophores. Dietary phytochemicals or their synthetic analogous as individual drug candidates or in combination with FDA approved chemotherapeutic drugs have exhibited potent anti-cancer efficacy. With the advancement in cancer therapeutics, dietary phytochemicals hold high prevalence for their use as precision and personalized medicine to replace conventional chemotherapeutic drugs. Hence, keeping these perspectives in mind, this review focuses on the diversity of dietary phytochemicals and their molecular mechanism of action in several cancer subtypes and tumor entities. Understanding the possible molecular key players involved, the use of dietary phytochemicals will thrive a new horizon in cancer therapy