J Clin Immunol

We report a longitudinal analysis of the immune response associated with a fatal case of COVID-19 in Europe. This patient exhibited a rapid evolution towards multiorgan failure. SARS-CoV-2 was detected in multiple nasopharyngeal, blood, and pleural samples, despite antiviral and immunomodulator treatment. Clinical evolution in the blood was marked by an increase (2–3-fold) in differentiated effector T cells expressing exhaustion (PD-1) and senescence (CD57) markers, an expansion of antibody-secreting cells, a 15-fold increase in γδ T cell and proliferating NK-cell populations, and the total disappearance of monocytes, suggesting lung trafficking. In the serum, waves of a pro-inflammatory cytokine storm, Th1 and Th2 activation, and markers of T cell exhaustion, apoptosis, cell cytotoxicity, and endothelial activation were observed until the fatal outcome. This case underscores the need for well-designed studies to investigate complementary approaches to control viral replication, the source of the hyperinflammatory status, and immunomodulation to target the pathophysiological response. The investigation was conducted as part of an overall French clinical cohort assessing patients with COVID-19 and registered in clinicaltrials.gov under the following number: NCT04262921

Impact of species and antibiotic therapy of enterococcal peritonitis on 30-day mortality in critical care - An analysis of the OUTCOMEREA database

Introduction: Enterococcus species are associated with an increased morbidity in intraabdominal infections (IAI). However, their impact on mortality remains uncertain. Moreover, the influence on outcome of the appropriate or inappropriate status of initial antimicrobial therapy (IAT) is subjected to debate, except in septic shock. The aim of our study was to evaluate whether an IAT that did not cover Enterococcus spp. was associated with 30-day mortality in ICU patients presenting with IAI growing with Enterococcus spp. Material and methods: Retrospective analysis of French database OutcomeRea from 1997 to 2016. We included all patients with IAI with a peritoneal sample growing with Enterococcus. Primary endpoint was 30-day mortality. Results: Of the 1017 patients with IAI, 76 (8%) patients were included. Thirty-day mortality in patients with inadequate IAT against Enterococcus was higher (7/18 (39%) vs 10/58 (17%), p = 0.05); however, the incidence of postoperative complications was similar. Presence of Enterococcus spp. other than E. faecalis alone was associated with a significantly higher mortality, even greater when IAT was inadequate. Main risk factors for having an Enterococcus other than E. faecalis alone were as follows: SAPS score on day 0, ICU-acquired IAI, and antimicrobial therapy within 3 months prior to IAI especially with third-generation cephalosporins. Univariate analysis found a higher hazard ratio of death with an Enterococcus other than E. faecalis alone that had an inadequate IAT (HR = 4.4 [1.3-15.3], p = 0.019) versus an adequate IAT (HR = 3.1 [1.0-10.0], p = 0.053). However, after adjusting for confounders (i.e., SAPS II and septic shock at IAI diagnosis, ICU-acquired peritonitis, and adequacy of IAT for other germs), the impact of the adequacy of IAT was no longer significant in multivariate analysis. Septic shock at diagnosis and ICU-acquired IAI were prognostic factors. Conclusion: An IAT which does not cover Enterococcus is associated with an increased 30-day mortality in ICU patients presenting with an IAI growing with Enterococcus, especially when it is not an E. faecalis alone. It seems reasonable to use an IAT active against Enterococcus in severe postoperative ICU-acquired IAI, especially when a third-generation cephalosporin has been used within 3 months. © 2019 The Author(s)

How do the geological and geophysical signatures of permeable fractures in granitic basement evolve after long periods of natural circulation? Insights from the Rittershoffen geothermal wells (France)

Abstract Two deep wells were drilled at Rittershoffen (Alsace, France) to produce high-temperature fluids to supply heat to a biorefinery. The GRT-2 production well was drilled to a depth of 3196 m MD and was deviated to target a permeable local fault in the granitic basement buried beneath a thick sedimentary cover. The objective of this study is to better understand the permeability of fractured reservoirs within crystalline rocks, focusing on the production well GRT-2. Based on a petrographic and mineralogical analysis of cutting samples, several granitic facies associated with hydrothermal alteration were identified on the basis of the amounts of illite, chlorite, anhydrite, secondary geodic quartz, and oxides. These observations were correlated with various geological and geophysical datasets (gamma ray, porosity, density, electrical resistivity, caliper, borehole image logs, temperature, rate of penetration, and mud losses) to localize and identify permeable fracture zones. In sections where acoustic image logs were not available, such as in the deepest part of the well, the geometries of the fracture zones were interpreted from an oriented caliper log. The caliper log interpretation detected one-third of the fractures detected by acoustic image logs. However, two major fracture sets striking N–S and dipping eastward or westward were observed. Furthermore, a synthetic resistivity log that fits the measured resistivity log relatively well was built using the Archie and Waxman and Smits models. This approach is a proxy for estimating the porosity and the mineralogical changes based on the cation exchange capacity, which is controlled by the chlorite/illite ratio, derived from electrical logs in granitic formations. The correlation of all these results allowed the identification of a resistivity signature of a permeable fracture zone that spatially fits with the temperature signature. The major contribution of this study is the identification of a hierarchy of permeable fractures based on petrophysical signatures. The geophysical signature of fracture zones with low residual permeability exhibits a broad depth extent, whereas the geophysical signature of a highly permeable fracture zone is more localized. Past hydrothermal circulation has enlarged the altered and porous zones around open fractures, and in some cases, intense illitization has plugged these fracture zones and reduced their permeabilities

Timing of geothermal activity in an active island-arc volcanic setting: preliminary 40Ar/39Ar data from Bouillante geothermal field (Guadeloupe, French West Indies),

International audienceMineral separates of adularia have been extracted from three samples of highly silicified hydrothermal breccias, newly discovered in the active Bouillante geothermal field (Guadeloupe archipelago), and investigated by 40Ar/39Ar geochronology in order to constrain the timing of geothermal activity in this part of the active Lesser Antilles island arc. The inverse isochron diagram indicates an age of 248±50 ka (2σ) for all adularia from one breccia sample (n=8), with an initial 40Ar/36Ar ratio of atmospheric composition (309±12 (2σ)) attesting that this age is valid. This age is concordant with the weighted mean age of 290±40 ka for the same sample. Adularia from other samples yields concordant ages. The obtained 40Ar/39Ar ages can be related either to the magmatic activity of the Bouillante Volcanic Chain (c. 850–250 ka ago) or to the initiation of the volcanic activity of the active Grande Découverte–Soufrière system (200 ka ago–present day). Our results demonstrate that the Bouillante hydrothermal event is coeval with change in the volcanic pulses previously recognized in the magmatic history of the studied area. The possible duration calculated for this hydrothermal activity requires at least two superposed volcanic pulses to be developed

Mineral assemblages and distribution of phyllosilicates composition along the main section of the Agua Rica deposit, Catamarca, Argentina. Implications for future mine development

International audienceAgua Rica (27 degrees 26'S-66 degrees 16'O) is a world class Cu (Mo-Au) deposit located in Catamarca, Argentina, in which the porphyry and high sulfidation epithermal stages usually distant 1 km vertically, are located at the same level of erosion. Seventy one samples and 120 phyllosilicates were analyzed from the study section (E-W 6969400 N) to determine distinctive mineralogical zones which differ not only by their hydrothermal history but also by their potential behavior during the ore processing operations via flotation. In the east side of the section, biotite K1.78-1.67 Na0.05-0.02(Al0.42-0.00Mg3.77-3.22Fe1.46-1.00Ti0.90-0.18Mn0.02-0.01) (Si5.42-4.82Al3.18-2.58) (OHF1.90-1.40Cl0.06-0.04)(4) of the early, high temperature potassic alteration (>550 degrees C to 370 degrees C) is encapsulated in a phyllic halo dominated by illite K1.73-1.08 Na0.10-0.0(Al3.96-3.49Mg0.36-0.03Fe0.25-0.01Ti0.06-0.0) (Si6.53-6.07Al1.93-1.47) (OHF0.54-0.0Cl0.02-0.0)(4) + quartz + pyrite + covellite + molybdenite + rutile. This mineral assemblage formed from fluids with temperatures <= 350 degrees C that attained high sulfur fugacity. The clasts of the hydrothermal and mineralized breccia bodies located at the center of the section and to the east contain widespread pyrophyllite K0.05-0.0(Al4.07-3.94Fe0.12-0.01)(Si7.92-7.50Al0.50-0.05)(OHF0.15-0.02Cl0.03-0.0)(4) that had replaced illite at temperatures between 360 degrees C and 280 degrees C. Minor amounts of dickite and widespread kaolinite occur in the advanced argillic and phyllic halos, respectively, as late minerals in clots or in veinlets thus, fluids cooled enough (<270 degrees and <200 degrees C, respectively) for their formation. The presence of phyllosilicate minerals is a potential cause of loss in recovery of copper and molybdenite during the flotation process. Carefully planned and controlled comprehensive liberation and flotation testing should be undertaken in conjunction with mineralogical analysis to optimize and quantify recoveries, separations, grades and gangue deportment

K-Ar dating and δ18O - δD tracing of illitization within and outside the Shea Creek uranium prospect, Athabasca Basin, Canada

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Oral probiotic treatment of Lactobacillus rhamnosus Lcr35 ® prevents visceral hypersensitivity to a colonic inflammation and an acute psychological stress

International audienceAims: This study evaluated the efficacy of a repeated oral treatment with two active pharmaceutical ingredients (Lcr Lenio (R) and Lcr Restituo (R)) derivated from the probiotic bacterial strain Lactobacillus rhamnosus Lcr35 (R) in two animal models mimicking different features of irritable bowel syndrome (IBS). IBS is characterized by visceral pain associated with alteration of bowel transit. IBS patients present visceral hypersensitivity with peripheral and central origins.Methods and Results: The injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) into the proximal colon as well as an acute partial restraint stress (PRS) produces colonic hypersensitivity measured in conscious rats by a decrease in pain threshold in response to distal colonic distension. Visceral hypersensitivity was produced by injection of TNBS 7 days before colonic distension or by acute PRS on testing day. Treatments were performed once a day during eight consecutive days.Conclusions: This study indicates that an 8-day probiotic treatment (Lcr Lenio and Lcr Restituo) produces an antihypersensitivity activity in both TNBS and PRS visceral pain models. As this probiotic strain attenuates peripherally and centrally induced visceral hypersensitivity in rats, it may be active in treatment of IBS symptoms. An immunomodulatory effect of the probiotics was highlighted in the TNBS model on the IL-23 secretion, suggesting a mechanism of action involving a regulation of the local IL-23/Th17 immune activation.Significance and Impact of the Study: Two formulas of Lcr35 (R) probiotic strain show very encouraging results for the treatment of IBS patients. Further studies are needed to better understand the role and mechanisms of probiotics on the pathogenesis of IBS

Genesis of the Loma Galena Pb-Ag deposit, Navidad District, Patagonia, Argentina: a unique epithermal system capped by an anoxic lake

Loma Galena (978,852 t Pb, 206 Moz Ag) is one of eight epithermal deposits in the world-class Navidad Pb + Ag ± (Zn, Cu) district located in the Cañadón Asfalto continental foreland basin, northern Patagonia, Argentina. This basin formed during the Jurassic in an extensional tectonic regime during the breakup of Gondwana. Host rocks comprise major listric faulted and tilted blocks of K-rich andesite to dacite lava flows (173.9–170.8 Ma; U-Pb ages for zircon) unconformably overlain by mudstone interbedded with stromatolitic and pisolitic limestones, sandstone, coal, and an Sr-rich evaporite layer deposited in a lacustrine environment. The mineralization occurs as disseminations in the organic-rich sedimentary rocks, in veins and hydrothermal breccia dikes in the hanging walls and footwalls of NW- and NE-striking normal faults, in volcanic autobreccias, and in a phreatic breccia at the contact of volcanic and sedimentary rocks. The earliest hydrothermal minerals consist of veins of colloform, crustiform, and cockade calcite 1 (δ13Cfluid –4.7 to 0.8‰; δ18Ofluid 4.8–11.6‰) and siderite. The precipitating fluids were likely basement-exchanged basinal brines having salinities of 9.5 to 16.4 wt % NaCl equiv and temperatures of 154.7° to 212°C. The interaction of these fluids with the host volcanic rocks formed calcite, albite, adularia, and celadonite-glauconite-group minerals followed by chlorite and siderite as fO2 decreased. Fluids intermittently boiled, as evidenced by bladed (platy) texture in calcite 1. Subsequent mineralizing stages contributed to the metal endowment of Loma Galena. The abundance of organic-rich mudstone and δ34S from –15.4 to 12.9‰ for sulfides suggests that the bottom waters of the lake were anoxic and the loci of microbial sulfate reduction (evaporites have δ34S 35‰). Mixing of upflowing metal-rich basinal fluids carrying some S from depth with this H2S-rich connate water efficiently precipitated Ag-bearing framboidal pyrite, colloform pyrite-marcasite, chalcopyrite, bornite, tennantite-tetrahedrite, sphalerite, and galena as veins, breccias, and disseminations in host rocks. The highest grade and tonnage of the ores are found in autobreccias at the junction of the uppermost lava flow and in the overlying mudstone, where the addition of a strong microbial signature is recorded in sulfides. This event also led to partial dissolution of magmatic and hydrothermal feldspar and calcite 1 in the altered volcanic rocks. Mineralization was followed by hydrothermal brecciation and successive precipitation of chalcedony (δ18Ofluid 2.6–4.8‰), barite (δ34S 15.7–22‰; 160.9°–183.8°C; 7.7–9.7 wt % NaCl equiv), calcite 2 (δ18Ofluid –10.2 to –3.7‰, 58°–95°C; 1.9–7.0 wt % NaCl equiv), strontianite, and quartz in brecciated veins and breccias; kaolinite (δ18Ofluid 2–6.2‰), illite-smectite, smectite, and carbonates with minor chalcedony and barite in the volcanic rocks; and calcite, chalcedony, and barite in the sedimentary rocks. A trend of decreasing salinity with decreasing temperature and lowering δ18O of the fluids with time suggests dilution of the basinal fluids by mixing with Jurassic meteoric water (δ18O −9 to −5.2‰). Loma Galena is a unique example of a polymetallic epithermal system formed in a sublacustrine anoxic environment that promoted the efficient deposition and preservation of Ag-bearing sulfides, thereby contributing to the large size and relatively high grade of the deposit