Fitting a square peg into a round hole: are the current Surviving Sepsis Campaign guidelines feasible for Africa?

In their article, Baelani and colleagues surveyed anesthesia providers from African low- and middle-income countries (LMICs) to evaluate whether or not the current Surviving Sepsis Campaign (SSC) guidelines are feasible in such resource-constrained settings. The authors report that an alarmingly low percentage of hospitals have the capacity to implement the SSC guidelines in their entirety but a higher percentage are able to implement the majority of SSC guidelines and grade 1 recommendations. In reality, the probability of adherence to SSC guidelines for septic management is even lower than reported, given that the majority of sepsis management in African LMICs is likely performed by non-intensivists outside of intensive care units. Efforts to address the challenges of managing severely ill patients in LMICs have recently been taken on by the World Health Organization. After reviewing available evidence for sepsis management predominantly from high-income countries, a panel of experts developed a consensus-based strategy tailored for resource-limited settings. However, more research that can evaluate the challenges specific to sepsis management in LMICs and not currently addressed by the SSC guidelines is needed. Comprehensive, evidence-based guidelines combined with innovative approaches to sepsis management in LMICs are required to make a meaningful impact on worldwide sepsis survival

Evidence for the Hierarchical Formation of the Galactic Spheroid

The possibility that the Galactic spheroid was assembled from numerous chemically-distinct, proto-Galactic fragments is investigated using a Monte-Carlo technique designed to simulate the chemical evolution of the Galaxy in hierarchical formation scenarios which involve no gas dissipation. By comparing the observed and simulated metallicity distributions of Galactic globular clusters and halo field stars, we estimate the level of fragmentation in the collapsing proto-Galaxy. Although the formation process is highly stochastic, the simulations often show good agreement with the observed metallicity distributions, provided the luminosity function of proto-Galactic fragments had a power-law form with exponent ~ -2. While this steep slope is strongly at odds with the presently observed luminosity function of the Local Group, it is in close agreement with the predictions of semi-analytic and numerical models of hierarchical galaxy formation. We discuss a number of possible explanations for this discrepancy. These simulations suggest that the Galactic halo and its globular cluster system were assembled via the accretion and disruption of approximately 1000 metal-poor, proto-Galactic fragments by the dominant Galactic building block: a proto-bulge whose own metal-rich globular clusters system has been preferentially eroded by dynamical processes. We argue that the same process (ie, hierarchical growth involving little gas dissipation) is responsible for the formation of both giant elliptical galaxies and the bulge-halo components of spiral galaxies. (ABRIDGED).Comment: 20 pages, 9 postscript figures. Accepted for publication in the ApJ, April 10 2000 issu

The ACS Virgo Cluster Survey. XIV. Analysis of Color-Magnitude Relations in Globular Cluster Systems

We examine the correlation between globular cluster (GC) color and magnitude using HST/ACS imaging for a sample of 79 early-type galaxies (-21.7<M_B<-15.2 mag) with accurate SBF distances from the ACS Virgo Cluster Survey. Using the KMM mixture modeling algorithm, we find a highly significant correlation, d(g-z)/dz = -0.037 +- 0.004, between color and magnitude for the subpopulation of blue GCs in the co-added GC color-magnitude diagram of the three brightest Virgo galaxies (M49, M87 and M60): brighter GCs are redder than their fainter counterparts. For the single GC systems of M87 and M60, we find similar correlations; M49 does not appear to show a significant trend. There is no correlation between (g-z) and M_z for GCs of the red subpopulation. The correlation d(g-z)/dg for the blue subpopulation is much weaker than d(g-z)/dz. Using Monte Carlo simulations, we attribute this to the fact that the blue subpopulation in M_g extends to higher luminosities than the red subpopulation, which biases the KMM fits. The correlation between color and M_z thus is a real effect. This conclusion is supported by biweight fits to the same color distributions. We identify two environmental dependencies of the color-magnitude relation: (1) the slope decreases in significance with decreasing galaxy luminosity; and (2) the slope is stronger for GCs at smaller galactocentric distances. We examine several mechanisms that might give rise to the observed color-magnitude relation: (1) presence of contaminators; (2) accretion of GCs from low-mass galaxies; (3) stochastic effects; (4) capture of field stars by individual GCs; and (5) GC self-enrichment. We conclude that self-enrichment and field-star capture, or a combination of these processes, offer the most promising means of explaining our observations.Comment: 15 pages, 12 figures, accepted for publication in the Astrophysical Journal. Uses emulateapj.cl

Genomic distribution of H3K9me2 and DNA methylation in a maize genome

DNA methylation and dimethylation of lysine 9 of histone H3 (H3K9me2) are two chromatin modifications that can be associated with gene expression or recombination rate. The maize genome provides a complex landscape of interspersed genes and transposons. The genome-wide distribution of DNA methylation and H3K9me2 were investigated in seedling tissue for the maize inbred B73 and compared to patterns of these modifications observed in Arabidopsis thaliana. Most maize transposons are highly enriched for DNA methylation in CG and CHG contexts and for H3K9me2. In contrast to findings in Arabidopsis, maize CHH levels in transposons are generally low but some sub-families of transposons are enriched for CHH methylation and these families exhibit low levels of H3K9me2. The profile of modifications over genes reveals that DNA methylation and H3K9me2 is quite low near the beginning and end of genes. Although elevated CG and CHG methylation are found within gene bodies, CHH and H3K9me2 remain low. Maize has much higher levels of CHG methylation within gene bodies than observed in Arabidopsis and this is partially attributable to the presence of transposons within introns for some maize genes. These transposons are associated with high levels of CHG methylation and H3K9me2 but do not appear to prevent transcriptional elongation. Although the general trend is for a strong depletion of H3K9me2 and CHG near the transcription start site there are some putative genes that have high levels of these chromatin modifications. This study provides a clear view of the relationship between DNA methylation and H3K9me2 in the maize genome and how the distribution of these modifications is shaped by the interplay of genes and transposons.The research was supported by a grant from the National Science Foundation (IOS-1237931) to MWV and NMS. This work also used resources or cyberinfrastructure provided by iPlant Collaborative. The iPlant Collaborative is funded by a grant from the National Science Foundation (DBI-0735191; www. iplantcollaborative.org). Start-up funds from the University of Georgia and a research grant from the National Science Foundation (IOS-1339194) to RJS supported aspects of this study

HIV-1 subtypes and drug resistance mutations among female sex workers varied in different cities and regions of the Democratic Republic of Congo

Background Complex mosaic structures of HIV-1 were found in the Democratic Republic of Congo (DRC). Currently, there is limited information on the circulating HIV-1 strains, the distribution of these strains and antiretroviral (ART) resistant viruses in different regions of the country, and the HIV-1 strains harbored by the high-risk groups like female sex workers (FSW) reported to be the source of recombinant and ART resistant viruses. Methods Dried Blood Spots (DBS), collected from 325 infected FSWs in ten cities from 2012 DRC HIV/STI Integrated Biological and Behavioral Surveillance Survey, were tested for HIV-1 genotypes and antiretroviral resistance mutations. Regional segregation of HIV-1 clades was detected using phylogenetics. The significance for differences in HIV-1 subtype and drug resistance mutations were evaluated using Chi-square tests. Results There were 145 (env) and 93 (pol) sequences analyzed. Based on env sequences, the predominant subtype was A1 (44%), and recombinants as defined pol sequences comprised 35% of the total sample. Paired sequences of pol and env from DRC FSW revealed mosaic recombinant in 54% of the sequences. Distinct geographic distributions of different HIV-1 subtypes and recombinants were observed. Subtype A1 was prevalent (40%) in Goma located in the East and significantly higher than in Mbuji-Mayi (p\u3c0.05) in the South-centra

Effects of CurraNZ, a New Zealand blackcurrant extract during 1 hour of treadmill running in female and male Marathon des Sables Athletes in hot conditions: two case studies

Four weeks before competition in the 2023 Marathon des Sables, a 6 stage, ~250 km running event in the Sahara Desert, we examined effects of 7-day intake of New Zealand blackcurrant extract (210 mg anthocyanins per day) on 1 h treadmill running-induced physiological and metabolic responses in the heat (~34°C, relative humidity: ~30%) in a non-acclimatized amateur female and male athlete (age: 23, 38 yrs, BMI: 24.2, 28.4 kg·m-2, body fat%: 29.2, 18.8%, V ̇O2max: 50.1, 52.1 mL·kg-1·min-1). During the 1 h run at 50%V ̇O2max (speed female: 7.3, male: 7.5 km·h-1), indirect calorimetry was used and heart rate recorded at 15-min intervals with core temperature monitoring (0.05 Hz). The 1 h runs were 3 h after a light breakfast and 2 h after intake of the final dose of New Zealand blackcurrant extract with water allowed ad libitum during the run. With the New Zealand blackcurrant extract, there were no effects in the female athlete. The respiratory exchange ratio (RER) of the female athlete in the non-supplement control condition was 0.77±0.01, indicating existing ~77% contribution of fat oxidation to the energy requirements. In the male athlete during 1 h of running, fat oxidation was higher by 21% (p<0.01), carbohydrate oxidation was 31% lower (p=0.05), RER was 0.03 units lower (p=0.04) and core temperature was 0.4°C lower (p<0.01) with no differences for heart rate, minute ventilation, oxygen uptake and carbon dioxide production for the New Zealand blackcurrant condition compared to the non-supplement control condition. Seven-day intake of New Zealand blackcurrant extract (210 mg anthocyanins per day) provided beneficial physiological and metabolic responses during exertional heat stress by 1 h of indoor (~34°C) treadmill running in a male Marathon des Sables athlete 4 weeks before competition. Future work is required to address whether New Zealand blackcurrant provide a nutritional ergogenic effect for Marathon des Sables athletes during long-duration running in the heat combined with personalized nutrition

Starspot-induced optical and infrared radial velocity variability in T Tauri star Hubble 4

We report optical (6150 Ang) and K-band (2.3 micron) radial velocities obtained over two years for the pre-main sequence weak-lined T Tauri star Hubble I 4. We detect periodic and near-sinusoidal radial velocity variations at both wavelengths, with a semi-amplitude of 1395\pm94 m/s in the optical and 365\pm80 m/s in the infrared. The lower velocity amplitude at the longer wavelength, combined with bisector analysis and spot modeling, indicates that there are large, cool spots on the stellar surface that are causing the radial velocity modulation. The radial velocities maintain phase coherence over hundreds of days suggesting that the starspots are long-lived. This is one of the first active stars where the spot-induced velocity modulation has been resolved in the infrared.Comment: Accepted for publication in The Astrophysical Journa

Employing Respondent Driven Sampling (RDS) to recruit people who inject drugs (PWID) and other hard-to-reach populations during COVID-19: Lessons learned

Background: Respondent Driven Sampling (RDS) is an effective sampling strategy to recruit hard-to-reach populations but the impact of the COVID-19 pandemic on the use of this strategy in the collection of data involving human subjects, particularly among marginalized and vulnerable populations, is not known. Based on an ongoing study using RDS to recruit and study the interactions between HIV infection, injection drug use, and the microbiome in Puerto Rico, this paper explores the eectiveness of RDS during the pandemic and provided potential strategies that could improve recruitment and data collection. Results: RDS was employed to evaluate its effectiveness in recruiting a group of people who inject drugs (PWID) and controls (N = 127) into a study in the midst of the COVID-19 pandemic. The participants were distributed among three subsets: 15 were HIV+ and PWID, 58 were HIV- PWID, and 54 were HIV+ and not PWID. Findings: Results showthat recruitment through peer networks using RDS was possible across all sub-groups. Yet, while those in the HIV+ PWID sub-group managed to recruit from other-sub groups of HIV- PWID and HIV+, this occurred at a lower frequency. Conclusion: Despite the barriers introduced by COVID-19, it is clear that even in this environment, RDS continues to play a powerful role in recruiting hard-to-reach populations. Yet, more attention should be paid at how future pandemics, natural disasters, and other big events might affect RDS recruitment of vulnerable and hard-to-reach populations

Source to sink: Evolution of lignin composition in the Madre de Dios River system with connection to the Amazon basin and offshore:Lignin evolution in Amazon

While lignin geochemistry has been extensively investigated in the Amazon River, little is known about lignin distribution and dynamics within deep, stratified river channels or its transformations within soils prior to delivery to rivers. We characterized lignin phenols in soils, river particulate organic matter (POM), and dissolved organic matter (DOM) across a 4 km elevation gradient in the Madre de Dios River system, Peru, as well as in marine sediments to investigate the source-to-sink evolution of lignin. In soils, we found more oxidized lignin in organic horizons relative to mineral horizons. The oxidized lignin signature was maintained during transfer into rivers, and lignin was a relatively constant fraction of bulk organic carbon in soils and riverine POM. Lignin in DOM became increasingly oxidized downstream, indicating active transformation of dissolved lignin during transport, especially in the dry season. In contrast, POM accumulated undegraded lignin downstream during the wet season, suggesting that terrestrial input exceeded in-river degradation. We discovered high concentrations of relatively undegraded lignin in POM at depth in the lower Madre de Dios River in both seasons, revealing a woody undercurrent for its transfer within these deep rivers. Our study of lignin evolution in the soil-river-ocean continuum highlights important seasonal and depth variations of river carbon components and their connection to soil carbon pools, providing new insights into fluvial carbon dynamics associated with the transfer of lignin biomarkers from source to sink

Hubble Space Telescope Observations of cD Galaxies and their Globular Cluster Systems

We have used WFPC2 on the Hubble Space Telescope to obtain F450W and F814W images of four cD galaxies (NGC 541 in Abell 194, NGC 2832 in Abell 779, NGC 4839 in Abell 1656 and NGC 7768 in Abell 2666) in the range 5400 < cz < 8100 km s^{-1}. For NGC 541, the HST data are supplemented by ground-based B and I images obtained with the FORS1 on the VLT. We present surface brightness and color profiles for each of the four galaxies, confirming their classification as cD galaxies. Isophotal analyses reveal the presence of subarcsecond-scale dust disks in the nuclei of NGC 541 and NGC 7768. Despite the extreme nature of these galaxies in terms of spatial extent and luminosity, our analysis of their globular cluster systems reveals no anomalies in terms of specific frequencies, metallicity gradients, average metallicities, or the metallicity offset between the globulars and the host galaxy. We show that the latter offset appears roughly constant at \Delta [Fe/H] ~ 0.8 dex for early-type galaxies spanning a luminosity range of roughly four orders of magnitude. We combine the globular cluster metallicity distributions with an empirical technique described in a series of earlier papers to investigate the form of the protogalactic mass spectrum in these cD galaxies. We find that the observed GC metallicity distributions are consistent with those expected if cD galaxies form through the cannibalism of numerous galaxies and protogalactic fragments which formed their stars and globular clusters before capture and disruption. However, the properties of their GC systems suggest that dynamical friction is not the primary mechanism by which these galaxies are assembled. We argue that cDs instead form rapidly, via hierarchical merging, prior to cluster virialization.Comment: 36 pages, 20 postscript figures, uses emulateapj. Accepted for publication in the Astronomical Journa