Songs From Wilhelm Tell

The following selections are translated from the German of the first three songs from Wilhelm Tell, by Friedrich Schiller

Terminal deoxynucleotidyltransferase. Serological studies and radioimmunoassay

Mouse antisera against calf terminal deoxynucleotidyltransferase (terminal transferase) have been prepared. The sera have been used to characterize terminal transferase both by studying inhibition of enzyme activity and by developing a competition radioimmunoassay using highly purified 125I-labeled terminal transferase. By either assay, anti-terminal transferase serum did not cross-react significantly with calf DNA polymerases alpha and beta, Escherichia coli DNA polymerase I, or the reverse transcriptase of Moloney mouse leukemia virus. The calf terminal transferase did, however, share cross-reactive but not identical determinants with human and murine terminal transferase. The radioimmunoassay could detect as little as 2 ng of terminal transferase/mg of soluble protein in a tissue extract. Thymocytes were found to contain 280 ng of terminal transferase/mg of cell protein or about 1 X 10^(5) molecules/cell; bone marrow had about 1% of the level of enzyme found in thymus. Extracts of spleen, peripheral white blood cells, lymph nodes, liver, muscle, and kidney all lacked detectable antigenicity of terminal transferase. These data indicate that terminal transferase is a tissue-specific enzyme and is not related to other DNA polymerases

Intercomparisons of GOES-derived cloud parameters and surface observations over San Nicolas Island

The spatial sampling limitations of surface measurement systems necessitate the use of satellite data for the investigation of large-scale cloud processes. Understanding the information contained in the satellite-observed radiances, however, requires a connection between the remotely sensed cloud properties and those more directly observed within the troposphere. Surface measurements taken during the First ISCCP Regional Experiment (FIRE) Marine Stratocumulus Intensive Field Observations (IFO) are compared here to cloud properties determined from Geostationary Operational Environmental Satellite (GOES) data in order to determine how well the island measurements represent larger areas and to verify some of the satellite-measured parameters

Contrasting Multiple Social Network Autocorrelations for Binary Outcomes, With Applications To Technology Adoption

The rise of socially targeted marketing suggests that decisions made by consumers can be predicted not only from their personal tastes and characteristics, but also from the decisions of people who are close to them in their networks. One obstacle to consider is that there may be several different measures for "closeness" that are appropriate, either through different types of friendships, or different functions of distance on one kind of friendship, where only a subset of these networks may actually be relevant. Another is that these decisions are often binary and more difficult to model with conventional approaches, both conceptually and computationally. To address these issues, we present a hierarchical model for individual binary outcomes that uses and extends the machinery of the auto-probit method for binary data. We demonstrate the behavior of the parameters estimated by the multiple network-regime auto-probit model (m-NAP) under various sensitivity conditions, such as the impact of the prior distribution and the nature of the structure of the network, and demonstrate on several examples of correlated binary data in networks of interest to Information Systems, including the adoption of Caller Ring-Back Tones, whose use is governed by direct connection but explained by additional network topologies

Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone

The mouse thymus contains two forms of terminal deoxynucleotidyl transferase (TdT) which are distinguishable by the salt concentration necessary to elute them from a phosphocellulose column, by their distrubtion among the thymocyte subpopulations, and by their sensitivity to cortisone treatment. In the whole thymus the later eluting peak (peak II) is the predominant one with about 3-10% of the total activity appearing in peak I. Both peak I and peak II activities are most sensitively assayed by the polymerization of dGMP onto an oligo(dA) primer. The minor population of thymocytes which is less dense and cortisone-resistant contains a higher specific activity of peak I TdT. The majority of TdT activity is, however, found in the major population of thymocytes which occurs in the center region of a bovine serum albumin gradient and is cortisone-sensitive. A very low level of an activity indistinguishable from peak II TdT activity is also detected in the mouse bone marrow. Other tissues, such as spleen, liver, heart, and brain lack detectable amounts of TdT activity

Fast-field cycling NMR is sensitive to the method of cross-linking in BSA gels

This work was supported by ARUK (grant number 19689).Non peer reviewedPublisher PD

Pharmacogenetics, Kinetics, and Dynamics for Personalized Medicine by DF Kisor, MD Kane, JN Talbot and JE Sprague

Pharmacogenomics is the study of genetic differences responsible for the variability in drug response among individual patients. Emerging from this new science is the development of diagnostic tools crucial for improving treatment strategies, as well as reducing drug side effects. As the importance of this science is being realized, the need for this discipline in pharmacy student training is becoming increasingly crucial to their professional development and training as healthcare professionals. As this discipline evolves, there is a clear need for tools that can enable lecturers to deliver this essential training; the current book review investigates aspects that are specific to the needs of the target audience and will explore the potential of ‘Pharmacogenetics, Kinetics, and Dynamics for Personalized Medicine’ in achieving this task

Improving Information and Performance in Grain Marketing: an Assessment of Current Market Information Systems, and Recommendations for Developing a Public Grain MIS

This paper focuses on one dimension which is at the core of all modern economies: how to provide private and public agents with timely and accurate information regarding current and expected future market conditions. Its objective is to show the need for a public market information system for agricultural markets in Ethiopia, and to present concrete proposals that can be reviewed, debated, and modified as necessary by the relevant Ethiopian authorities in collaboration with Food Security Research Project (FSRP) personnel.food security, food policy, Crop Production/Industries, Marketing, Q18,