Development of primary invasive pneumococcal disease caused by serotype 1 pneumococci is driven by early increased type I interferon response in the lung

The pneumococcus is the world's foremost respiratory pathogen, but the mechanisms allowing this pathogen to proceed from initial asymptomatic colonization to invasive disease are poorly understood. We have examined the early stages of invasive pneumococcal disease (IPD) by comparing host transcriptional responses to an invasive strain and a noninvasive strain of serotype 1 Streptococcus pneumoniae in the mouse lung. While the two strains were present in equal numbers in the lung 6 h after intranasal challenge, only the invasive strain (strain 1861) had invaded the pleural cavity at that time point; this correlated with subsequent development of bacteremia in mice challenged with strain 1861 but not the noninvasive strain (strain 1). Progression beyond the lung was associated with stronger induction of the type I interferon (IFN-I) response in the lung at 6 h. Suppression of the IFN-I response through administration of neutralizing antibody to IFNAR1 (the receptor for type I interferons) led to significantly reduced invasion of the pleural cavity by strain 1861 at 6 h postchallenge. Our data suggest that strong induction of the IFN-I response is a key factor in early progression of invasive serotype 1 strain 1861 beyond the lung during development of IPD

Issues and challenges facing school libraries in selected primary schools in Gauteng Province, South Africa

There is no national policy for school libraries which compels school governing bodies and principals to have a library in their schools. It is thus not surprising that in 2011, only 21% of state schools had libraries, only 7% had stocked libraries and 79% of schools had no library at all (Department of Basic Education (DBE) Republic of South Africa, 2011a:23). This article forms part of a broader qualitative study which investigated 10 primary schools in Gauteng Province, South Africa that had libraries, or were in the process of setting up a library, with the intention of providing a rich description of the issues and challenges facing these schools. The 10 schools used in this study were chosen on the basis of their location and fee structures. They represented the full continuum of fees within the state structure. In this article, we discuss three core categories of predicaments faced by primary school libraries. These were resourcing the library, operating the library and the role of the library. Our main contention is that school libraries are not playing an effective role in supporting and enabling quality education for all South African children. This needs to be addressed by government as a matter of urgency.Keywords: academic achievement; books; information and communication technologies; information literacy; literacy; national school library policy; quality education; reading; school librarians; school librarie

Overlapping functionality of the Pht proteins in zinc homeostasis of streptococcus pneumoniae

Streptococcus pneumoniae is a globally significant pathogen that causes a range of diseases, including pneumonia, sepsis, meningitis, and otitis media. Its ability to cause disease depends upon the acquisition of nutrients from its environment, including transition metal ions such as zinc. The pneumococcus employs a number of surface proteins to achieve this, among which are four highly similar polyhistidine triad (Pht) proteins. It has previously been established that these proteins collectively aid in the delivery of zinc to the ABC transporter substrate-binding protein AdcAII. Here we have investigated the contribution of each individual Pht protein to pneumococcal zinc homeostasis by analyzing mutant strains expressing only one of the four pht genes. Under conditions of low zinc availability, each of these mutants showed superior growth and zinc accumulation profiles relative to a mutant strain lacking all four genes, indicating that any of the four Pht proteins are able to facilitate delivery of zinc to AdcAII. However, optimal growth and zinc accumulation in vitro and pneumococcal survival and proliferation in vivo required production of all four Pht proteins, indicating that, despite their overlapping functionality, the proteins are not dispensable without incurring a fitness cost. We also show that surface-attached forms of the Pht proteins are required for zinc recruitment and that they do not contribute to defense against extracellular zinc stress

Interaction of crustal heterogeneity and lithospheric processes in determining passive margin architecture on the southern Namibian margin

The influence of pre-rift crustal heterogeneity and structure on the evolution of a continental rift and its subsequent passive margin is explored. The absence of thick Aptian salts in the Namibian South Atlantic allows imaging of sufficient resolution to distinguish different pre-rift basement seismic facies. Aspects of the pre-rift basement geometry were characterized and compared with the geometries of the Cretaceous rift basin structure and with subsequent post-rift margin architectural elements. Half-graben depocentres migrated westwards within the continental synrift phase at the same time as basin-bounding faults became established as hard-linked arrays with lengths of c. 100 km. The rift–drift transition phase, marked by seaward-dipping reflectors, gave way to the early post-rift progradation of clastic sediments off the Namibian coast. In the Late Cretaceous, these shelf clastic sediments were much thicker in the south, reflecting the dominance of the newly formed Orange River catchment as the main entry point for sediments on the South African–Namibian margin. Tertiary clastic sediments largely bypassed the pre-existing shelf area, revealing a marked basinwards shift in sedimentation. The thickness of post-rift megasequences does not vary simply according to the location of synrift half-graben and thinned continental crust. Instead, the Namibian margin exemplifies a margin influenced by a complex interplay of crustal thinning, pre-rift basement heterogeneity, volcanic bodies and transient dynamic uplift events on the evolution of lithospheric strain and depositional architecture

Microstructural penetrometry of asteroid regolith analogues and Titan’s surface

We investigate essential aspects of penetrometer design required to measure particle properties on asteroids using a combination of laboratory analogue regoliths and spaceflight data returned by the Huygens probe from Titan. Penetrometry in granular material is complicated due to multiple and interdependent mechanical processes that occur during penetration. A numerical impact model is developed that simulates the behaviour of a penetrometer and its force sensor in a granular medium. The model is based on the Huygens ACC-E instrument that successfully returned penetrometry data from the surface of Titan. Penetrometry measurements are made in analogue asteroid regoliths using a laboratory copy of ACC-E. The average particle size in the targets ranged from 0.1 to 0.9 of the penetrometer tip diameter. To describe the structure seen in the data a number of metrics are defined to characterise the peaks seen in the returned signal. The significance of the variation of the metrics (such as peak height or width) with particle mass and radius are analysed in terms of penetrometer properties such as impact velocity, elastic properties and data logging parameters. We find the penetrometer can be used to measure average particle radius and mass adequately for a mid-range of particle radii. Electronic noise effects mostly the results from very small and very large particles. For high mass particles there is evidence that particle–particle impacts, within the target are being felt by the tip that make any straight forward interpretations using peak frequency a challenge. Using our numerical model the Huygens penetrometry data from Titan is analysed. A particle diameter, of around half a centimetre, is found to be consistent with the penetrometry data returned by Huygens. Recommendations and lessons learned, regarding data interpretation techniques are made for asteroid penetrometry (or any other extraterrestrial surface) when using this instrument

Investigating thermal properties of gas-filled planetary regoliths using a thermal probe

We introduce a general purpose penetrator, fitted with a heater, for measuring temperature and thermal diffusivity. Due to its simplicity of deployment and operation the penetrator is well suited for remote deployment by spacecraft into a planetary regolith. Thermal measurements in planetary regoliths are required to determine the surface energy balance and to measure their thermal properties. If the regolith is on a planet with an atmosphere a good understanding of the role of convection is required to properly interpret the measurements. This could also help to identify the significant heat and mass exchange mechanisms between the regolith and the atmosphere. To understand the role of convection in our regolith analogues we use a network of temperature sensors placed in the target. In practical applications a penetrator will push material out of the way as it enters a target possible changing its thermal properties. To investigate this effect a custom built test rig, that precisely controls and monitors the motion of the penetrator, is used. The thermal diffusivity of limestone powder and sand is derived by fitting a numerical thermal model to the temperature measurements. Convection seems to play an important role in the transfer of heat in this case. Firstly a diffusion-convection model fits the laboratory data better than a diffusivity-only model. Also the diffusivity derived from a diffusivity-convection model was found to be in good agreement with diffusivity derived using other methods published in the literature. Thermal diffusivity measurements, inspection of the horizontal temperature profiles and visual observations suggests that limestone powder is compacted more readily than sand during entry of the penetrator into the target. For both regolith analogues the disturbance of material around the penetrator was determined to have an insignificant effect on the diffusivity measurements in this case

The first histidine triad motif of phtd is critical for zinc homeostasis in Streptococcus pneumoniae

Streptococcus pneumoniae is the world's foremost human pathogen. Acquisition of the first row transition metal ion zinc is essential for pneumococcal colonization and disease. Zinc is acquired via the ATP-binding cassette transporter AdcCB and two zinc-binding proteins, AdcA and AdcAII. We have previously shown that AdcAII is reliant upon the polyhistidine triad (Pht) proteins to aid in zinc recruitment. Pht proteins generally contain five histidine (His) triad motifs that are believed to facilitate zinc binding and therefore play a significant role in pneumococcal metal ion homeostasis. However, the importance and potential redundancy of these motifs have not been addressed. We examined the effects of mutating each of the five His triad motifs of PhtD. The combination of in vitro growth assays, active zinc uptake, and PhtD expression studies show that the His triad closest to the protein's amino terminus is the most important for zinc acquisition. Intriguingly, in vivo competitive infection studies investigating the amino- and carboxyl-terminal His triad mutants indicate that the motifs have similar importance in colonization. Collectively, our new insights into the contributions of the individual His triad motifs of PhtD, and by extension the other Pht proteins, highlight the crucial role of the first His triad site in zinc acquisition. This study also suggests that the Pht proteins likely play a role beyond zinc acquisition in pneumococcal virulence

The Traits that Predict the Magnitude and Spatial Scale of Forest Bird Responses to Urbanization Intensity

As humans continue moving to urban areas, there is a growing need to understand the effects of urban intensification on native wildlife populations. Forest species in remnant habitat are particularly vulnerable to urban intensification, but the mechanisms behind these effects are poorly understood. An understanding of how species traits, as proxies for mechanisms, mediate the effects of urban intensification on forest species can help fill this knowledge gap. Using a large point count dataset from the Second Pennsylvania Breeding Bird Atlas, we tested for the effects of species traits on the magnitude and spatial scale of the responses of 58 forest bird species to urbanization intensity in landscapes surrounding count locations. Average urbanization intensity effect size across species was -0.36 ± 0.49 (SE) and average scale of effect of urbanization intensity was 4.87 ± 5.95 km. Resident forest bird species that are granivorous or frugivorous, cavity-nesting, and have larger clutch sizes and more fledglings per clutch had more positive associations with increasing urbanization intensity in landscapes. In addition, the effect of urbanization intensity on forest birds manifested most strongly at larger spatial scales for granivorous, frugivorous, or omnivorous species that are cavity-nesting, have larger clutch sizes and longer wingspans, and flock in larger numbers. To our knowledge, the present study represents the first direct tests of the effects of species traits on both the magnitude and spatial scale of the effect of urbanization on forest birds, as well as the first evidence that migratory status, clutch size, wingspan, and fledglings per clutch are important determinants of the responses of forest birds to urbanization. We discuss the possible mechanisms underlying our results and their implications for forest bird conservation in urbanizing landscapes