RITU (SEASON), RITUCHARYA AND PRAKRITI- A CONCEPTUAL STUDY

The Prakriti (genetic constitution) and Kala (season) are extra ordinarily related with each other from the origin of a Prakriti due to many common and mutual factors. Naturally the effect of Kala on the Prakriti is unavoidable. The off springs from the conjugation of same Sukra (sperm) and Shonita (ovum) are not alike by Prakriti, because the time of conception or fertilization of the ovum might be different. The prominent Doshas during the time of conception has got definite role in the formation of Prakriti during conception, as well as after birth also. If the time predominates the Vata dosha, there must be certain qualities of Vata Dosha, even though the conjugating Sukra and Shonita may be of different Dosha. In such cases the chances of mixed Prakriti is more. The Charakacharya said that, a chance of Sama prakriti find is very difficult or rare. Most of the Prakrities are Vatala, Pittala and Sleshmala with predominance of Vata, Pitta and Kapha respectively. The season has great role in the genetic constitution because the fertilization of ovum also depends upon the time. Sushruta said, after the cessation of the Rajo darshana (menstrual period) the 12 nights are useful for fertilization only. The modern physiology also states that the mature ovum get discharged through one of the two ovaries, every month, on or around the 14th day of cycle and so the period round about the 14th day is best for definite conception. This clearly shows the relation of Kala (season) and Prakriti (genetic constitution) since its origin and again ahead throughout the life time. Nowadays, the nature is changing its qualities due to various atmospheric changes like pollution, space experiments, heavy industrialization and population crisis. So while studying the effects of Kala (season) one has to face the hyper/ hypo and perverted faculties of Kala (season) which are affecting the generations. So the study of Kala (season) in respect of its various effects on Prakriti (genetic constitution), will be the essential thing in future. As per Ayurveda, Ritucharya (seasonal lifestyle) should be followed for prevention of diseases arising from variations in Tridosha due to Kala (season). In present study, we tried to advocate Ritucharya (seasonal lifestyle) with respect to Prakriti (genetic constitution)

Performance evaluation of a manually operated paddy drum seeder - a cost saving technology for paddy cultivation

 A study was conducted at farmer field to evaluate performance of mechanized paddy cultivation (T1), mechanized paddy cultivation with incorporation of Dhaincha before direct sowing of paddy seed (T2) and the traditional farmer practice (T3).  Consecutive three years of data were obtained for analysis and different crop growth parameters were measured like panicle length, number of tillers, grain yield etc. for each treatment.  It was found that the average grain yield for three years in different treatments T1 and T2 was enhanced by 10% and 14% respectively when compared with farmer practice.  Average cost of cultivation was reduced by 25% in treatment T2 where green manuring crop (Dhaincha) was grown and incorporated in soil with indigenous plough before paddy seeding.  Study also revealed that due to mechanized cultivation practices, the crop was ready to harvest eight to ten days early than farmer’s practices.  The experiment well proved to raise the socio-economic status of the farmers by changing their strategy towards mechanized organic paddy cultivation.   Keywords: drum seeder, mechanization, green manuring crop, growth parameters

Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urine

Background: The measurement of gene expression in post-digital rectal examination (DRE) urine specimens provides a non-invasive method to determine a patient’s risk of prostate cancer. Many currently available assays use whole urine or cell pellets for the analysis of prostate cancer-associated genes, although the use of extracellular vesicles (EVs) has also recently been of interest. We investigated the expression of prostate-, kidney-, and bladder- specific transcripts and known prostate cancer biomarkers in urine EVs. Methods: Cell pellets and EVs were recovered from post-DRE urine specimens, with the total RNA yield and quality determined by Bioanalyzer. The levels of prostate, kidney, and bladder- associated transcripts in EVs were assessed by TaqMan qPCR and targeted sequencing. Results: RNA was more consistently recovered from the urine EV specimens, with over 80% of the patients demonstrating higher RNA yields in the EV fraction as compared to urine cell pellets. The median EV RNA yield of 36.4 ng was significantly higher than the median urine cell pellet RNA yield of 4.8 ng. Analysis of the post-DRE urine EVs indicated that prostate-specific transcripts were more abundant than kidney- or bladder-specific transcripts. Additionally, patients with prostate cancer had significantly higher levels of the prostate cancer-associated genes PCA3 and ERG. Conclusions: Post-DRE urine EVs are a viable source of prostate-derived RNAs for biomarker discovery and prostate cancer status can be distinguished from analysis of these specimens. Continued analysis of urine EVs offers the potential discovery of novel biomarkers for pre- biopsy prostate cancer detection

epiCaPture: a urine DNA methylation test for early detection of aggressive prostate cancer

Purpose Liquid biopsies that noninvasively detect molecular correlates of aggressive prostate cancer (PCa) could be used to triage patients, reducing the burdens of unnecessary invasive prostate biopsy and enabling early detection of high-risk disease. DNA hypermethylation is among the earliest and most frequent aberrations in PCa. We investigated the accuracy of a six-gene DNA methylation panel (Epigenetic Cancer of the Prostate Test in Urine [epiCaPture]) at detecting PCa, high-grade (Gleason score greater than or equal to 8) and high-risk (D'Amico and Cancer of the Prostate Risk Assessment] PCa from urine. Patients and Methods Prognostic utility of epiCaPture genes was first validated in two independent prostate tissue cohorts. epiCaPture was assessed in a multicenter prospective study of 463 men undergoing prostate biopsy. epiCaPture was performed by quantitative methylation-specific polymerase chain reaction in DNA isolated from prebiopsy urine sediments and evaluated by receiver operating characteristic and decision curves (clinical benefit). The epiCaPture score was developed and validated on a two thirds training set to one third test set. Results Higher methylation of epiCaPture genes was significantly associated with increasing aggressiveness in PCa tissues. In urine, area under the receiver operating characteristic curve was 0.64, 0.86, and 0.83 for detecting PCa, high-grade PCa, and highrisk PCa, respectively. Decision curves revealed a net benefit across relevant threshold probabilities. Independent analysis of two epiCaPture genes in the same clinical cohort provided analytical validation. Parallel epiCaPture analysis in urine and matched biopsy cores showed added value of a liquid biopsy. Conclusion epiCaPture is a urine DNA methylation test for high-risk PCa. Its tumor specificity out-performs that of prostate-specific antigen (greater than 3 ng/mL). Used as an adjunct to prostate-specific antigen, epiCaPture could aid patient stratification to determine need for biopsy

A Study of Wireless Sensor Networks to Comprehend their Relevance to Different Applications

Wireless sensor networks (WSNs) have experienced enormous growth, both in terms of the technology used and their practical applications. In order to understand the features of WSNs that make the solution suitable for a specific purpose, one needs to be aware of the theoretical concepts behind and technological aspects of WSNs. In this paper, the significance of WSNs is illustrated, with a particular emphasis placed on their demands and on understanding researchrelated problems. A review of the literature available is presented as well. Detailed discussions concerning sensor node architecture, different types of sensors used and their relevance for various types of WSNs is presented, highlighting the need to achieve application-specific requirements without degrading service quality. Multipath and cluster-based routing protocols are compared in order to analyze QoS requirements they are capable of satisfying, and their suitability for different application areas is reviewed. This survey highlights the performance of different routing protocols, therefore providing guidelines enabling each of the routing techniques to be used, in an efficient manner, with factors such as specific network structure, protocol operation and routing path construction taken into consideration in order to achieve better performance

Differentiated Service Model-Supported Cluster-Based Routing in Wireless Sensor Networks

Wireless Sensor Network finds its extensive use in healthcare applications for the transfer of time-critical data through wireless connectivity. The primary cause of network failure is the transfer of time-critical multimedia data. The article presents a new dfferentiated service modelsupported (DSM) cluster-based routing in wireless sensor networks (WSNs) that overcomes the above issue. DSM prioritizes the transfer of dfferent flow types based on packet type and packet size. The employment of computational offlading minimizes delay for critical and small-sized data packets and by carrying out data reduction of large-sized packets at proxy server. It outperforms the existing protocols in terms of energy efficiency, throughput, and reliability by prioritizing the transfer of time-critical health application data

To evaluate anti-nociceptive activity of Aloe vera gel extract in graded doses in Albino rats by using radiant heat induced pain by using Analgesiometer

Background: Pain is a reaction of the body to harmful stimuli and is therefore a protective early warning system, the sensation of pain in postoperative patients, cancer patients, and other chronic pain patients has little positive effect. Objective: To evaluate anti-nociceptive activity of Aloe vera gel extract in graded doses in Albino rats by using radiant heat induced pain by using Analgesiometer. Materials and methods:The present study is undertaken to evaluate the anti-nociceptive effect of Aloe vera in animal models of pain in Albino rats. It was conducted in the Department of Pharmacology, Kamineni Institute of Medical Sciences (KIMS) from October 2012 - September 2014.Results:Aloe vera leaf gel extract powder dissolved in distilled water was evaluated for anti-nociceptive activity in tail flick method in albino rats. Tramadol, the known synthetic opioid analgesic is used as standard drug for comparing the effects of Aloe vera. Aloe vera in the doses of 200mg/kg and 400mg/kg orally produced significant anti-nociceptive effect in tail flick method in comparison to control group (DW). The standard drug tramadol in the dose of 20mg/kg orally also produced significant anti-nociceptive effect in tail flick method in comparison to control group (DW).Conclusion: Aloe vera leaf gel extract powder (dissolved in distilled water) possesses anti-nociceptive activity at 200 mg/kg and 400 mg/kg dosage at 60, 90 and 120 minutes when given orally in tail flick, model in albino rats

Hypogonadism is independently associated with varicocele repair in a contemporary cohort of men in the USA

We aimed to identify demographic and clinical predictors of varicocele repair in a contemporary cohort of men in the USA. We queried the 2009–2015 MarketScan Database using relevant ICD9, ICD10, and CPT codes to identify all 18–45 year olds with varicoceles. Differences in age, area of residence, clinical characteristics, and medical management between men who did and did not undergo varicocelectomy (open, laparoscopic, or microsurgical) during the study period were compared using unpaired t-tests and Chi-squared tests for continuous and categorical variables, respectively. Multivariable logistic regression analysis was used to evaluate age, semen analyses, and serum hormone assessment as predictors of varicocele repair. SAS version 9.4 was used for all statistical analyses. Significance was set at P < 0.05. Approximately 40% of men with varicoceles underwent repair, primarily through an open approach. Men who underwent repair were more likely to have a diagnosis of male infertility (15.5% vs 7.9%, P < 0.001) and male hypogonadism (3.4% vs 0.9%) and were more likely to complete semen analyses (36.1% vs 12.2%, P < 0.001) and serum testosterone evaluation (42.5% vs 18.8%, P < 0.001). In multivariable regression models, the strongest predictors of varicocele repair were semen analysis (OR = 2.78, 95% CI: 2.56–3.02), age 18–25 years (OR = 2.66, 95% CI: 2.36–2.98), and serum testosterone evaluation (OR = 1.67, 95% CI: 1.51–1.86). Although male infertility remains the most important indication for varicocele repair, male hypogonadism is emerging as an independent predictor of varicocelectomy, which may represent a change in the clinical management of varicoceles in the USA