Performance of the Sysmex XN-V hematology analyzer in determining the immature platelet fraction in dogs : A preliminary study and reference values

Altres ajuts: acords transformatius de la UABBackground: Immature platelets (IPs) are newly formed platelets released into circulation that have been demonstrated as good markers of thrombopoiesis. Although many flow cytometric and fully automated-based methods are available, the latest Sysmex XN-V hematology analyzer for veterinary use is equipped with a specific fluorescent platelet channel (PLT-F) that detects platelets using a platelet-specific dye. Objectives: The aims of this study were to evaluate the performance of the Sysmex XSN-1000 V in determining the IPF (immature platelet fraction) and other selected PLT-F channel parameters and to propose IPF reference intervals (RIs) for canine blood samples. Methods: Canine EDTA blood samples were analyzed on the Sysmex XN-1000 V to assess linearity, imprecision, carryover, stability, and the effect of platelet clumping on selected platelet parameters from the PLT-F channel. We also reported the de novo generated RIs for the IPF in dogs. Results: Imprecision was acceptable (CV <10%) for all parameters except for the absolute IPF values (IPF#), in which the reproducibility was 12.15% for the normal-low concentration samples. Linearity and carryover were excellent for all variables. Relative IPF values (IPF %) and IPF# remained stable for both storage conditions for up to 48 hours; however, a nonsignificant progressive increase in these parameters was observed from 12 hours at 4°C. We observed a statistical increase in IPF% and IPF# and a statistically significant decrease in PLT-F counts after intentional in vitro platelet aggregation. RIs were generated for all reference samples (n = 69) and for samples with (n = 25) or without (n = 44) platelet clumps. Conclusions: The performance of the new PLT-F channel-derived variables for dogs was excellent. Specific RIs for IPF should be used when platelet aggregates are present

Síndrome de feminización en un perro con un tumor testicular de células de Sertoli

Un perro de 8 años, criptórquido unilateral, se presenta con signos de disuria, alteraciones cutáneas y una masa en abdomen caudal. La ecografía determina la presencia de un quiste prostático y un posible testículo tumoral. Después de la cirugía se realiza histopatología que confirma la preencia de un tumor testicular de cálulas de Sertoli.

Laringitis granulomatosa en un perro

Perra mestiza de 13 años de edad con un historial de disnea aguda, debida a una obstrucción laríngea causada por una laringitis granulomatosa. Enfermedad de causa desconocida, posiblemente similar a la sarcoidosis humana

Hematologic and serum chemistry values of the captive european wildcat

Hematologic and serum chemistry values were determined for 20 adult captive European wildcats (Felis silvestris) in Lleida (Catalonia, Spain). Seven wildcats (4 females and 3 males) were captured in the wild and 13 (4 females and 9 males) were born and raised in captivity. Samples were collected between September and December from 1993 to 1998. Blood was obtained by jugular venipuncture after administration of either ketamine and xylazine or ketamine and medetomidine. Females had significantly higher mean eosinophil counts, albumin concentration, (and A/G ratio) and lower mature neutrophil counts, although these differences were not clinically relevant. Results for many of the blood parameters fall within the reference range for domestic cats. Mean values for alanine aminotransferase, aspartate aminotransferase, creatine phosphokinase, and lactate dehydrogenase activities as well as blood urea nitrogen, glucose, and sodium concentrations were higher than the upper limit of the reference range for domestic cat

Evaluación del analizador hematológico de impedancia eléctrica BAKER 9120+ con sangre de perro y gato

El objetivo de este trabajo es evaluar el analizador hematológico automático de impedancia eléctrica, Baker 9120+ (Menarini Diagnóstica S.A.), con sangre de perro y gato. La precisión intra e interensayo y la reproducibilidad del Baker 9120+ es excelente para todos los parámetros en ambas especies. El arrastre y la linealidad del analizador hematológico es muy buena para todos los parámetros, a excepción del recuento de plaquetas con sangre felina, debido a la sobreposición de la población eritrocitaria y plaquetar. No es posible determinar las diferentes poblaciones leucocitarias en ninguna de las dos especies, independientemente de la cantidad de hemolizante utilizado. La comparación del Baker 9120+ con otro analizador de impedancia eléctrica en el recuento celular y concentración de hemoglobina, y con el método manual para el valor hematocrito, es buena para todos los parámetros. El recuento de eritrocitos, leucocitos y la concentración de hemoglobina son estables durante las 72 horas postextracción, tanto a temperatura ambiente como en refrigeración. El recuento de plaquetas en sangre de perro desciende rápidamente con diferencias significativas a las 4 horas postextracción en las muestras refrigeradas y a las 24 horas en las muestras mantenidas a temperatura ambiente.An authomated blood cell analyser (Baker 9120+) was evaluated with canine and feline blood. Precision and reproducibility were excelent in both species for all the parameters. Carry-over and linearity was very good for all the parameters, except for the platelet count due to the overlapping between erythrocyte and platelet population. There was not possible to differenciate any of the leukocyte populations with the Baker 9120+. The comparability between the Baker 9120+ and another electric impedance analyzer for blood cell count and hemoglobin concentration and the microhematocrit method for the haematocrit value was good for all the parameters. Red blood cell, white blood cell count and haemoglobin concentration are stable during 72 hours after the extraction at 4 ºC and 25 ºC.Canine platelet count dropped significantly at 4 hours post- extraction in those samples stored at 4 "C and after 24 hours in those samples at room temperature

Systematic review of animal-based indicators to measure thermal, social, and immune-related stress in pigs

The intense nature of pig production has increased the animals' exposure to stressful conditions, which may be detrimental to their welfare and productivity. Some of the most common sources of stress in pigs are extreme thermal conditions (thermal stress), density and mixing during housing (social stress), or exposure to pathogens and other microorganisms that may challenge their immune system (immune-related stress). The stress response can be monitored based on the animals' coping mechanisms, as a result of specific environmental, social, and health conditions. These animal-based indicators may support decision making to maintain animal welfare and productivity. The present study aimed to systematically review animal-based indicators of social, thermal, and immune-related stresses in farmed pigs, and the methods used to monitor them. Peer-reviewed scientific literature related to pig production was collected using three online search engines: ScienceDirect, Scopus, and PubMed. The manuscripts selected were grouped based on the indicators measured during the study. According to our results, body temperature measured with a rectal thermometer was the most commonly utilized method for the evaluation of thermal stress in pigs (87.62%), as described in 144 studies. Of the 197 studies that evaluated social stress, aggressive behavior was the most frequently-used indicator (81.81%). Of the 535 publications examined regarding immune-related stress, cytokine concentration in blood samples was the most widely used indicator (80.1%). Information about the methods used to measure animal-based indicators is discussed in terms of validity, reliability, and feasibility. Additionally, the introduction and wide spreading of alternative, less invasive methods with which to measure animal-based indicators, such as cortisol in saliva, skin temperature and respiratory rate via infrared thermography, and various animal welfare threats via vocalization analysis are highlighted. The information reviewed was used to discuss the feasible and most reliable methods with which to monitor the impact of relevant stressors commonly presented by intense production systems on the welfare of farmed pigs

Reference Range of Kaolin-Activated Thromboelastography (TEG) Values in Healthy Pet Rabbits (Oryctolagus cuniculus)

Coagulation is a protective mechanism that prevents blood loss. The prothrombin time and activated partial thromboplastin clotting time are the most used laboratory tests used to assess coagulation status in all mammals. However, these tests do not accurately reflect the in vivo coagulation process due to complex interactions between blood cells and coagulation elements. New cell-based models incorporate the role of cells to explain the formation/destruction of the blood clot through viscoelastic laboratory techniques, such as thromboelastography (TEG). Thromboelastography reference ranges have been determined for humans, dogs, cats, horses, rats, mice, and laboratory rabbits but they have not been previously defined in pet rabbits. The aim of the article is to establish the reference-range values for TEG parameters in pet rabbits. Included in this study were 24 healthy rescue pet rabbits. A TEG 5000 Thromboelastograph Hemostasis System with citrated kaolin-activated blood was used. The reference ranges obtained for reaction time (R) were 1.4-6.9 min; clot formation time (K) was 0.8-2.2 min; α-angle was 65.8-82.2 degrees; maximal amplitude (MA) was 53.7-73.5 mm; measure of clot strength/firmness (G-value) was 5796.6-13,885.9 dyn/cm 2 ; the percentage of clot lysis in 30 min (LY30%) was 0-41.5%. This study provides the reference ranges of TEG in pet rabbits. Thromboelastography (TEG) is a viscoelastic technique that allows the examination of both cellular and plasma protein clotting factors. Thromboelastography helps to investigate the underlying coagulopathy and to monitor therapeutic modalities. Although viscoelastic techniques have been used in human and veterinary medicine, reference ranges in pet rabbits are missing. The objective of this study is to establish the reference-range values of TEG parameters in healthy pet rabbits. 24 healthy pet rabbits of different breeds were included: 16 crossbreeds, four Californians, two lops, one lionhead, and one angora. Four rabbits were less than one year old and 20 were older than one year. Twelve rabbits were neutered females, 10 neutered males, and two were intact females. Health status was assessed through a physical examination, a complete blood work, and a coagulation profile. A TEG 5000 Thromboelastograph Hemostasis System was used with kaolin-activated citrated whole blood. All samples were analysed 30 min postextraction. The TEG reference ranges were reaction time (R) 1.4-6.9 min; clot formation time (K) 0.8-2.2 min; α angle 65.8-82.2 degrees; maximal amplitude (MA) 53.7-73.5 mm; measure of clot strength/firmness (G-value) 5796.6-13,885.9 dyn/cm 2 ; and percentage of clot lysis in 30 min (LY30%) 0-41.5%. This study provides the reference ranges of TEG in pet rabbits