Muography applied to nuclear waste storage sites

Legacy storage sites for nuclear waste can pose a serious environmental problem. In fact, since certain sites date from the middle of the last century when safety protocols had not been properly established and strict bookkeeping was not enforced, a situation has evolved where the content of storage silos is basically known only with a large uncertainty both on quantity and quality. At the same time maintenance work on old storage structures is becoming ever more urgent and yet this work requires exactly that information which is now lacking on the type of waste that was stored inside. Because of the difficulty in accessing the storage silos and the near impossibility of making visual inspections inside, techniques have to be developed which can determine the presence or absence of heavy elements (i.e. uranium) within the structures. Muography is a very promising technique which could allow the survey of previously inaccessible structures. We have begun an evaluation performing feasibility studies using simulations based on real case scenarios. This paper will outline the storage site scenarios and then present some of the results obtained from the Monte Carlo simulations

Plasma proteins containing damaged L-isoaspartyl residues are increased in uremia: Implications for mechanism

Plasma proteins containing damaged L-isoaspartyl residues are increased in uremia: Implications for mechanism.BackgroundSeveral alterations of protein structure and function have been reported in uremia. Impairment of a transmethylation-dependent protein repair mechanism possibly related to a derangement in homocysteine metabolism is also present in this condition, causing erythrocyte membrane protein damage. Homocysteine may affect proteins via the accumulation of its parent compound S-adenosylhomocysteine (AdoHcy), a powerful in vivo methyltransferase inhibitor. However, since plasma homocysteine is mostly protein bound, a direct influence on protein structures cannot be ruled out. We measured the levels of L-isoaspartyl residues in plasma proteins of uremic patients on hemodialysis. These damaged residues are markers of molecular age, which accumulate when transmethylation-dependent protein repair is inhibited and/or protein instability is increased.MethodsL-isoaspartyl residues in plasma proteins were quantitated using human recombinant protein carboxyl methyl transferase (PCMT). Plasma concentrations of homocysteine metabolites were also measured under different experimental conditions in hemodialysis patients.ResultsThe concentration of damaged plasma proteins was increased almost twofold compared to control (controls 147.83 ± 17.75, uremics 282.80 ± 26.40 pmol of incorporated methyl groups/mg protein, P < 0.003). The major protein involved comigrated with serum albumin. Although hyperhomocysteinemia caused a redistribution of thiols bound to plasma proteins, this mechanism did not significantly contribute to the increase in isoaspartyl residues. The S-adenosylmethionine (AdoMet)/AdoHcy concentration ratio, an indicator of the flux of methyl group transfer, was altered. This ratio was partially corrected by folate treatment (0.385 ± 0.046 vs. 0.682 ± 0.115, P < 0.01), but protein L-isoaspartate content was not.ConclusionsPlasma protein damage, as determined by protein L-isoaspartyl content, is increased in uremia. This alteration is to be ascribed to an increased protein structural instability, rather than the effect of hyperhomocysteinemia

Role of perineural invasion as a prognostic factor in laryngeal cancer

The diffusion of laryngeal cancer cells in the perineural space is a parameter associated with a negative prognosis, high loco-regional recurrence and low disease-free survival rates. The spread of tumor cells on the perineural sheath highlights the histopathological and clinically aggressive behavior of this type of tumor, which may extend proximally or distally in the nerve for >10 cm. Therefore, the surgical resection margin is generally insufficient to treat patients with laryngeal cancer presenting with perineural invasion (PNI) with surgery alone. In PNI, the minor laryngeal nerves are frequently involved, rather than the superior and inferior laryngeal nerves. The aim of the present study was: i) To evaluate the prognostic importance of PNI; ii) to correlate the rate of infiltration with factors associated with the tumor, including histotype, site and tumor-node-metastasis stage, and with the type of surgery (total or partial laryngectomy); and iii) to evaluate the rate of disease-free survival according to the outcome of combined surgery and radiotherapy (RT) treatment, by means of retrospective analysis. The results of the present study highlighted the importance of performing a closer clinical and instrumental follow-up in patients with laryngeal cancer whose histopathological examination is positive for PNI. In such cases, it is important to complement the surgical therapeutic treatment with adjuvant RT

Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor

Periprostatic adipose tissue (PPAT) has emerged as a key player in the prostate cancer (PCa) microenvironment. In this study, we evaluated the ability of PPAT to promote PCa cell migration, as well as the molecular mechanisms involved. Methods: We collected conditioned mediums from in vitro differentiated adipocytes isolated from PPAT taken from PCa patients during radical prostatectomy. Migration was studied by scratch assay. Results: Culture with CM of human PPAT (AdipoCM) promotes migration in two different human androgen-independent (AI) PCa cell lines (DU145 and PC3) and upregulated the expression of CTGF. SB431542, a well-known TGFβ receptor inhibitor, counteracts the increased migration observed in presence of AdipoCM and decreased CTGF expression, suggesting that a paracrine secretion of TGFβ by PPAT affects motility of PCa cells. Conclusions: Collectively, our study showed that factors secreted by PPAT enhanced migration through CTGF upregulation in AI PCa cell lines. These findings reveal the potential of novel therapeutic strategies targeting adipocyte-released factors and TGFβ/CTGF axis to fight advanced PCa dissemination

Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging

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a possible point of contact between cosmic ray physics and archaeology muon absorption radiography at the tharros phoenician roman site

Several on-going activities exploiting the potential of the muon-absorption radiography technique are focusing on the study of large volcanic structures located in different geographical regions. Nonetheless, the possible application of this non-destructive surveying method to other fields is also under consideration by different groups. Looking backward to the history of muon radiography, we can learn how the first important success of this technique was achieved in the archaeological field by the physicist Luis W. Alvarez in the 1960s. Further examples of possible applications can be found concerning geological and mining applications. In this paper a possible application of muon absorption radiography in the context of the archaeological research is proposed. Results of a simulation of a simplified case study are presented

a geant4 framework for generic simulations of atmospheric muon detection experiments

A flexible and adaptive simulation framework based on Geant4 is presented. The framework has been developed in order to speed up the deployment of full-featured Monte Carlo simulation codes for small/medium sized particle detection experiments with generic geometries. Specific components related to atmospheric muon experiments have been developed and are presented as well

The MURAVES muon telescope: technology and expected performances

The MURAVES project aims to study the inner structure of the upper part of the Mt. Vesuvius volcano by muon radiography (muography) technique. Very high energy muons, produced by cosmic rays in the at- mosphere, can penetrate large thickness of rocks. By measuring the at- tenuation of the muons flux trough the volcano cone is possible to obtain a 2D image of the density structure. Internal discontinuities, with a spa- tial resolution of about 10 m, can be, in principle, resolved. An absolute average density measurement can be provided too. The project, funded by the Italian Ministry of University, Research and Education (MIUR), is led by INGV and INFN. In this article the mechanical structure of the de- tectors and background suppression techniques are reported

Association between Vitamin D Receptor Gene Polymorphisms and Periodontal Bacteria: A Clinical Pilot Study

Abstract: Background: Periodontitis is an inflammatory disease caused by microorganisms involving the supporting tissues of the teeth. Gene variants may influence both the composition of the biofilm in the oral cavity and the host response. The objective of the study was to investigate the potential correlations between the disease susceptibility, the presence and the quantity of periodontopathogenic oral bacterial composition and the VDR gene polymorphisms. Methods: Fifty (50) unrelated periodontal patients and forty-one (41) healthy controls were selected for genomic DNA extraction. DNA concentration was measured and analyzed. The periodontopathogenic bacterial species were identified and quantified using a Real Time PCR performed with species-specific primers and probes. Results: Genotype distribution showed a different distribution between the groups for BsmI rs1544410 genotypes (p = 0.0001) with a prevalence of the G(b) allele in periodontal patients (p = 0.0003). Statistical significance was also found for VDR TaqI rs731236 (p ≤ 0.00001) with a prevalence of the T(T) allele in periodontal patients (p ≤ 0.00001). The average bacterial copy count for the periodontitis group was significantly higher than that of control group. Dividing patients into two groups based on high or low bacterial load, FokI rs2228570 T allele (f) was statistically more represented in patients with high bacterial load. Conclusions: The findings of the study suggest the involvement of the VDR gene BsmI and TaqI polymorphisms in periodontal disease, while FokI and BsmI may be involved in determining an increased presence of periodontopathogens