The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids.

Telomerase is an enzyme that adds repetitive DNA sequences to the ends of chromosomes and consists of two main subunits: the telomerase reverse transcriptase (TERT) protein and an associated telomerase RNA (TER). The telomerase essential N-terminal (TEN) domain is a conserved region of TERT proposed to mediate DNA substrate interactions. Here, we have employed single molecule telomerase binding assays to investigate the function of the TEN domain. Our results reveal telomeric DNA substrates bound to telomerase exhibit a dynamic equilibrium between two states: a docked conformation and an alternative conformation. The relative stabilities of the docked and alternative states correlate with the number of basepairs that can be formed between the DNA substrate and the RNA template, with more basepairing favoring the docked state. The docked state is further buttressed by the TEN domain and mutations within the TEN domain substantially alter the DNA substrate structural equilibrium. We propose a model in which the TEN domain stabilizes short RNA-DNA duplexes in the active site of the enzyme, promoting the docked state to augment telomerase processivity

The governance of wolves in transboundary regions: a triquetrous study of ephemeral agreements transcending sub-national and national boundaries

Thesis (M.S.) University of Alaska Fairbanks, 2013Contradictory management objectives in adjacent jurisdictions can affect transboundary wolves and their associated socio-ecological systems. Elite interviews and case study methodology were used in this thesis to explore three transboundary wolf management agreements, their effectiveness, and their impacts on wolves, ecosystems and stakeholders. Separate agreements between the State of Alaska and: Yukon-Charley Rivers National Preserve, and Denali National Park and Preserve, and an agreement between Italy and Switzerland show that despite a diversity of socio-ecological contexts, approaches, and hierarchical level of actors, transboundary wolf agreements are prone to ephemerality. The ephemerality of these agreements appears to be due primarily to institutional path dependency, and to political tension between management entities. The impacts of these agreements and their cessation, on socio-ecological systems are limited by the agreements' limited scopes. The agreements do however figure incrementally into larger trends, especially including changes in rural and urban identities, and in large carnivore management discourse. I argue that a diversity of wolf management approaches across a landscape, and the inherent conflict between management entities preserves adaptive capacity by preventing one size fits all prescriptions based on incomplete knowledge. Assuming no acute state of emergency, incremental rather than transformational change is more equitable to diverse stakeholders; allowing public perception, policy, and scientific knowledge to shift concurrently. The cases also suggest that facilitating trans-entity conversation and coordination at multiple levels would support understanding, and increase the prevalence of creative agreements contributing to amenable, incremental change. Landscape Conservation Cooperatives are put forth as a potential platform or template for this facilitation

Sports Literature for Struggling Secondary Readers

Exploring the topic of how to hook struggling readers at the secondary level. For many boys who struggle with reading, engaging them in sports literature is a way to appeal to the interest of a large number of adolescent males. The creation of a sports literature class would supplement the instruction in the regular English Language Arts class and would open the window into reading via a variety of texts

On the Curved Patterns Seen in the Graph of PPTs

Dr. Ron Knott constructed a graph of all Primitive Pythagorean Triples (PPTs) with legs up to length 10,000, using Mathematica. The patterns are very interesting, suggesting conic sections. We show that they indeed are parabolic curves which follow in a natural way from the mathematics of the subject matter.Comment: 8 pages, 10 figure

HIV/AIDS and behavioral healthcare in rural settings

Presentation at the 6th Annual Rural HIV Research and Training Conferenc

The Whirlwind Passeth (May 4, 1951)

Program for The Whirlwind Passeth (May 4, 1951). To view the photos from this production of The Whirlwind Passeth, please click here

Computing Pythagorean Triples

Pythagorean triples are the positive integer solutions to the Pythagoras equation for right triangles, a2+b2 = c2. They have been studied for many years, many centuries in fact. In this short paper we present a method for computing Pythagorean triples in general, the first two cases of which go back at least to the early Pythagoreans (570-495 BCE), and to Plato (429-347 BCE). But the method has not been investigated as extensively as presented here. Several other approaches are also considered.Comment: 17 pages, 16 figure