Self-consistent triaxial de Zeeuw-Carollo Models

We use the usual method of Schwarzschild to construct self-consistent solutions for the triaxial de Zeeuw & Carollo (1996) models with central density cusps. ZC96 models are triaxial generalisations of spherical $\gamma$-models of Dehnen whose densities vary as $r^{-\gamma}$ near the center and $r^{-4}$ at large radii and hence, possess a central density core for $\gamma=0$ and cusps for $\gamma > 0$. We consider four triaxial models from ZC96, two prolate triaxials: $(p, q) = (0.65, 0.60)$ with $\gamma = 1.0$ and 1.5, and two oblate triaxials: $(p, q) = (0.95, 0.60)$ with $\gamma = 1.0$ and 1.5. We compute 4500 orbits in each model for time periods of $10^{5} T_{D}$. We find that a large fraction of the orbits in each model are stochastic by means of their nonzero Liapunov exponents. The stochastic orbits in each model can sustain regular shapes for $\sim 10^{3} T_{D}$ or longer, which suggests that they diffuse slowly through their allowed phase-space. Except for the oblate triaxial models with $\gamma =1.0$, our attempts to construct self-consistent solutions employing only the regular orbits fail for the remaining three models. However, the self-consistent solutions are found to exist for all models when the stochastic and regular orbits are treated in the same way because the mixing-time, $\sim10^{4} T_{D}$, is shorter than the integration time, $10^{5} T_{D}$. Moreover, the ``fully-mixed'' solutions can also be constructed for all models when the stochastic orbits are fully mixed at 15 lowest energy shells. Thus, we conclude that the self-consistent solutions exist for our selected prolate and oblate triaxial models with $\gamma = 1.0$ and 1.5.Comment: 6 Pages, 3 Figures, 2 Tables. Accepted for Publication in A&

An approach to identify issues affecting ERP implementation in Indian SMEs

Purpose: The purpose of this paper is to present the findings of a study which is based on the results of a comprehensive compilation of literature and subsequent analysis of ERP implementation success issues in context to Indian Small and Medium scale Enterprises (SME’s). This paper attempts to explore the existing literature and highlight those issues on ERP implementation and further to this the researchers applied TOPSIS (Technique for order preference by similarity to ideal solution) method to prioritize issues affecting successful implementation of ERP. Design/methodology/approach: Based on the literature review certain issues leading to successful ERP implementation have been identified and to identify key issues Pareto Analysis (80-20 Rule) have been applied. Further to extraction of key issues a survey based on TOPSIS was carried out in Indian small and medium scale enterprises. Findings: Based on review of literature 25 issues have been identified and further Pareto analysis has been done to extract key issues which is further prioritized by applying Topsis method. Research limitations/implications: Beside those identified issues there may be other issues that need to be explored. There is scope to enhance this study by taking into consideration different type of industries and by extending number of respondents. Practical implications: By identifying key issues for SMEs, managers can better prioritize issues to make implementation process smooth without disruption. ERP vendors can take inputs from this study to change their implementation approach while targeting small scale enterprises. Originality/value: There is no published literature available which followed a similar approach in identification of the critical issues affecting ERP in small and mid-sized companies in India or in any developing economyPeer Reviewe

Lactobacillus acidophilus Derived Biosurfactant as a Biofilm Inhibitor: A Promising Investigation Using Microfluidic Approach

Background: Biomedical devices and implants are adversely affected by biofilm-associated infections that pose serious public health issues. Biosurfactants (BSs) can combat pathogenic biofilms through their antimicrobial, antibiofilm and antiadhesive capabilities. The objective of our research was to produce biosurfactant (BS) from Lactobacillus acidophilus NCIM 2903 and investigate its antibiofilm, antiadhesive potential using microfluidics strategies by mimicking the micro-environment of biofilm. Methods: Antibiofilm and antiadhesive potential was effectively evaluated using different methods like microfluidics assay, catheter assay, polydimethlysiloxane (PDMS) disc assay. Along with this chemical and physical characteristics of BS were also evaluated. Results: Cell free biosurfactant (CFBS) obtained was found to be effective against biofilm which was validated through the microfluidic (MF) or Lab on Chip (LOC) approach. The potency of CFBS was also evaluated on catheter tubing and PDMS surfaces (representative bioimplants). The efficacy of CFBS was also demonstrated through the reduction in surface tension, interfacial tension, contact angle and low critical micelle concentration. Conclusion: CFBS was found to be a potent antimicrobial and antibiofilm agent. We believe that perhaps this is the first report on demonstrating the inhibiting effect of Lactobacillus spp. derived CFBS against selected bacteria via LOC approach. These findings can be explored to design various BSs based formulations exhibiting antimicrobial, antibiofilm and antiadhesive potential for biomedical applications

Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant

Abstract Background Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903. Results Extracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6 mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane - PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains. Conclusion The current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices

A family of triaxial modified Hubble mass models: effects of the additional radial functions

The projected properties of triaxial generalization of the modified Hubble mass models are studied. These models are constructed by adding the additional radial functions, each multiplied by a low-order spherical harmonic, to the models of \citet{ct00}. The projected surface density of mass models can be calculated analytically which allows us to derive the analytic expressions of axial ratio and position angles of major axis of constant density elliptical contours at asymptotic radii. The models are more general than those studied earlier in the sense that the inclusions of additional terms in density distribution, allows one to produce varieties of the radial profile of axial ratio and position angle, in particular, their small scale variations at inner radii. Strong correlations are found to exist between the observed axial ratio evaluated at $0.25 R_{e}$ and at $4 R_{e}$ which occupy well-separated regions in the parameter space for different choices of the intrinsic axial ratios. These correlations can be exploited to predict the intrinsic shape of the mass model, independent of the viewing angles. Using Bayesian statistics, the result of a test case launched for an estimation of the shape of a model galaxy is found to be satisfactory.Comment: 23 pages, 8 figures, accepted for publication in New Astronom

Screening of Aeromonads as associated pathogens from Non-Tuberculous Mycobacterial infections in the aquaculture industry, West Bengal, India

The disease termed as ‘Ulcerative disease’ or ‘Erythematous disease’ is found in fishes and fish handlers and is caused by a group of waterborne Mycobacterium spp. called non-tuberculous mycobacteria (NTM). The wounds are frequently invaded by secondary pathogens like Aeromonas spp. which is ubiquitous in nature. NTMs are free-living bacterium inhabiting water bodies, causing skin lesions in fish and fish handlers. The secondary invaders are natural inhabitants and are found in excess due to anthropogenic pollution in aquatic environments affecting the fishes and human subjects as low-level latent infectants in wounds caused by NTM. This study highlights the various aspects mycobacteriosis followed by secondary infection and hemorrhagic septicemia caused by Aeromonas spp. in the state of West Bengal (WB), India. NTM and Aeromonas spp. samples were examined from different districts of WB. In the case of Fish handlers, NTM infection as well as Aeromonas spp. infected wounds were highly significant (correlation coefficient (ρ) 0.859, p<0.01). Ulcerative fishes predominant with NTMs were significantly higher in the total samples studied (correlation coefficient 0.718, p<0.01) than the fishes infected with both Aeromonas spp. and NTM (ρ 0.188, p< 0.5). Systematic reporting of mycobacteriosis and associated pathogens studied here will help to improvise the drug regimes used in culture-based fisheries systems

Active emulsions in living cell membranes driven by contractile stresses and transbilayer coupling

The spatiotemporal organisation of proteins and lipids on the cell surface has direct functional consequences for signaling, sorting and endocytosis. Earlier studies have shown that multiple types of membrane proteins including transmembrane proteins that have cytoplasmic actin binding capacity and lipid-tethered GPI-anchored proteins (GPI-APs) form nanoscale clusters driven by active contractile flows generated by the actin cortex. To gain insight into the role of lipids in organizing membrane domains in living cells, we study the molecular interactions that promote the actively generated nanoclusters of GPI-APs and transmembrane proteins. This motivates a theoretical description, wherein a combination of active contractile stresses and transbilayer coupling drive the creation of active emulsions, mesoscale liquid ordered (lo) domains of the GPI-APs and lipids, at temperatures greater than equilibrium lipid-phase segregation. To test these ideas we use spatial imaging of homo-FRET combined with local membrane order and demonstrate that mesoscopic domains enriched in nanoclusters of GPI-APs are maintained by cortical actin activity and transbilayer interactions, and exhibit significant lipid order, consistent with predictions of the active composite model

Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production

Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current research deals with isolation of Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water for BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach to explore the genomic insight of the strain SAMP and displayed the presence of gene for BS biosynthesis. The genome analysis revealed this potential to be intrinsic to the strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) and oil displacement (OD) showed SAMP MCC 3013 as a potent BS producer. BS reduced SFT of phosphate buffer saline (PBS) pH: 7.0 from 72 to 30 mN/m with a critical micelle concentration (CMC) value of 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (1H NMR and 13C NMR) and liquid chromatography mass spectrometry (LC-MS) revealed terpene containing BS having sugar, lipid moieties. The genomic sequence analysis of P. maritimus SAMP showed complete genes in the pathway for the synthesis of terpenoid. Probably terpenoid is the accountable backbone molecule for the BS production, but the later stages of terpenoid conversion to the BS could not be found. Moreover, it is important to highlight that till today; no single report documents the in-detailed physico-chemical characterization of BS from Planococcus sp. Based on genomic and functional properties, the term terpene containing BS is denoted for the surfactant produced by P. maritimus