Role of Vitamin D and Vitamin D Receptor in Oral Lichen Planus: A Systematic Review

BACKGROUND: Oral lichen planus (OLP) is known to be a chronic inflammatory disease associated with various other systemic disorders. Studies have shown that vitamin D deficiency can be involved in the pathogenesis of lichen planus. The aim of this study was to investigate the role of vitamin D and vitamin D receptor in OLP.METHODS: In this review study, all English and Persian articles were searched by relevant keywords from the Google scholar, PubMed, science direct, Cochrane, Scopus and Sid databases until January 2020.RESULTS: From the 16 articles obtained after reviewing the abstracts, finally 14 appropriate articles were included in this study.CONCLUSION: According to the results of the studies, vitamin D deficiency may be associated with an increased risk of OLP lesions

Role of interleukin-4 in pathogenesis of oral lichen planus : a systematic review

Oral lichen planus (OLP) is a premalignant mucocutaneous disease that affects 1-2% of the adult population. Immunological factor may act as etiological factor. The cellular immune cells such as T cells are important in pathogenesis of OLP. Interleukin-4 (IL-4) is secreted by T-helper 2 (Th2). Several studies have been carried out on the role of IL-4 in OLP. The aim of this study was to review the level of IL-4 in OLP, effective factors in the production of IL-4 and its role in the development of OLP. A search in PubMed was performed on the literature published from 2000 until august 2019 using the following keywords: ?oral lichen planus? or ?OLP? and ?interleukin-4? or ?IL-4?. Originally, 37 articles were considered, of which 28 case-control articles were selected according to the inclusion/exclusion criteria. This review study shows that IL-4 plays a key role in the development of OLP. According to the past studies, there are several factors contributing to the production of this cytokine. Identification of the routes of production of IL-4 and its role in OLP might be useful for development of new preventive and therapeutic methods in management of patients with OLP

Salivary Vascular Endothelial Growth Factor and Epidermal Growth Factor Levels in Patients with Recurrent Aphthous Stomatitis: a Meta-Analysis

Recurrent aphthous stomatitis (RAS) has been identified as a common oral lesion with an unknown pathogenesis. Various studies have been conducted to show the important role of two factors named epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) in RAS, but certain results have not been achieved. The present meta-analysis was conducted to evaluate the salivary levels of EGF and VEGF in patients with RAS. For this purpose, the related articles in the Web of Science, PubMed, Embase, ProQuest and Scopus databases until January 2022 were searched and their abstracts were studied. Google scholar and scientific information database were also searched for articles in Persian. The searches were completed by the medical subject heading terms considering "recurrent aphthous stomatitis" and "saliva" in combination with "EGF" or "VEGF" keywords. All case control studies that evaluated the salivary levels of EGF and VEGF in patients with RAS were included in this study. To evaluate statistical heterogeneity between the studies, Cochrane Q and I2 tests were adopted. The extracted data then were used in the analysis process based on comprehensive meta-analysis software. Originally, 619 articles were considered, of which 7 articles were selected. According to this meta-analysis, salivary EGF and VEGF levels were significantly lower in the active and remission period of RAS than in healthy individuals (p Value< 0.05). In addition, salivary levels of these factors were significantly lower in the active stage of RAS than in the healing phase. This review study suggests that decreasing of salivary EGF and VEGF levels have significant role in the development of RAS

Comparing the effects of lycopene and corticosteroids on oral lichen planus: a systematic review and meta-analysis

Oral lichen planus (OLP) as an inflammatory disease is autoimmune in nature and there is no definitive treatment for it. Currently, topical and systemic corticosteroids have been adopted as the gold standard in the treatment of OLP, which have significant side effects. Nowadays, the tendency towards natural medicines with antioxidant properties as an alternative treatment has increased due to their low side effects. The main purpose of this systematic review and meta-analysis was to focus on comparing the effects of lycopene as an herbal intervention and corticosteroids on OLP in clinical trials. The search using keywords was done through provided databases from PubMed, Web of Science, Google Scholar, and Scopus until March 2023. After removing duplicate articles and screening them according to eligibility criteria, 5 articles remained and were considered for systematic review and meta-analysis. According to the meta-analysis, significant difference was not observed between the lycopene and corticosteroid groups in terms of reducing pain and improving clinical symptoms after treatment. Lycopene was tolerated well and no specific adverse effects were shown. The systematic review results showed the significant effect of lycopene in the treatment of OLP. The results of this meta-analysis can be useful because no significant side effects have been specified for lycopene. Hence, it might be used instead of steroids

Therapeutic Fc fusion protein misfolding: A three-phasic cultivation experimental design.

Cell culture process optimization is a critical solution to most of the challenges faced by the pharmaceutical manufacturing. One of the major problems encountered in large-scale production of therapeutic proteins is misfolded protein production. The accumulation of misfolded therapeutic proteins is an immunogenic signal and a risk factor for immunogenicity of the final product. The aim of this study was the statistical optimization of three-phasic temperature shift and timing for enhanced production of correctly folded Fc-fusion protein. The effect of culture temperatures were investigated using the biphasic culture system. Box-Behnken design was then used to compute temperature and time of shifting optimum. Response surface methodology revealed that maximum production with low level of misfolded protein was achieved at two-step temperature shift from 37°C to 30°C during the late logarithmic phase and 30°C to 28°C in the mid-stationary phase. The optimized condition gave the best results of 1860 mg L-1 protein titer with 24.5% misfolding level. The validation experiments were carried out under optimal conditions with three replicates and the protein misfolding level was decreased by two times while productivity increased by ~ 1.3-fold. Large-scale production in 250 L bioreactor under the optimum conditions was also verified the effectiveness and the accuracy of the model. The results showed that by utilizing two-step temperature shift, productivity and the quality of target protein have been improved simultaneously. This model could be successfully applied to other products