Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins

My dissertation hypothesis is that bovine trophoblast cells express cell-surface and secreted non-classical major histocompatibility complex class I (MHC-Ib) proteins which inhibit NK cells and other leukocytes by binding to inhibitory receptors (e.g., LILRB1, LILRB2, KIR2DL4, and/or CD94/NKG2A). Extremely polymorphic and ubiquitously expressed classical MHC class I (MHC-Ia) proteins, which present foreign antigenic peptides to CD8+ T lymphocytes, are involved in acceptance or rejection of tissue grafts. Non-classical MHC class I (MHC-Ib) glycoproteins, such as Human Leukocyte Antigen-G (HLA-G) and murine Qa-2, are important modulators of the maternal immune system during pregnancy. MHC-Ib proteins are: (a) oligomorphic or monomorphic, (b) expressed in specific tissues under specific condtions, and (c) produced as surface and/or soluble isoforms due to alternative splicing. Third trimester-bovine trophoblast cells express both MHC-Ia and MHC-Ib proteins. The MHC-Ib proteins expressed by trophoblast cells during the third trimester of pregnancy are encoded by four bovine leukocyte antigen (BoLA) loci: BoLA-NC1, BoLA-NC2, BoLA-NC3, and BoLA-NC4. Two MHC-Ia (N*01701 and N*01802) and three MHC-Ib (NC1*00501, NC3*00101 and NC4*00201) proteins showed cell-surface expression in transfection studies performed in murine P815 and human K562 cells. Two additional isoforms, NC1*00401 and NC2*00102, were not detected on the surface of these cells. Nevertheless, both class Ia proteins, N*01701 and N*01802, and five class Ib proteins, NC1*00401, NC1*00501, NC2*00102, NC3*00101, and NC4*00201, were detected in crude cell lysates on Western blots. Precipitation of proteins from culture supernatants showed that cell-surface MHC-Ia (N*01701 and N*01802) and MHC-Ib proteins (NC1*00501, NC3*00101, and NC4*00201) are shed from the surface of these cells into the media. The mechanism of shedding of these proteins is, however, not known. Monoclonal antibodies W6/32, IL-A88, H1A, H6A, H11A, H58A, and PT-85A recognized surface MHC-I isoforms with varying affinity. We were able to develop a sandwich enzyme-linked immunosorbent assay (ELISA) using either H1A or IL-A88 antibody as the capture antibody and the W6/32 antibody for detection. We produced monoclonal antibodies against cattle NC1*00501 and NC3*00101 proteins. One monoclonal antibody generated against BoLA-NC3*00101 was highly specific. Unfortunately, due to failure to clone the NC3*00101- hybridoma, we no longer have an infinite source of this monoclonal antibody for NC3*00101. We eluted peptides from NC3*00101-transfected MHC-null K562 cells and identified peptides using liquid chromatography-mass spectrum (LC-MS) analysis. Analysis of peptide binding data using the SAS Proc mixed statistical program, suggested that the peptide EVTNQLVVL is a potential peptide ligand, which can be used to make tetramers for enumeration of antigen-specific leukocytes

Food Distribution Value Chains under the Integrated Child Development Services

Globally, social provisioning of food is recognised as an important means to reduce the prevalence of malnutrition. Government food distribution programmes have potential for impact at scale in this context. This article examines the food distribution value chain of the Supplementary Nutrition Programme (SNP) under the Integrated Child Development Services (ICDS) scheme of the Government of India, targeted at pregnant and lactating women and children below six years of age. Following the conceptual framework outlined in Maestre, Poole, and Henson (2017), the article examines two different models in operation under the SNP value chain in two states of India: Telangana, where a state enterprise is engaged in the manufacture of fortified premix for distribution, and Tamil Nadu, which follows a public–private partnership (PPP) model. The article highlights the challenges and opportunities associated with the two models and attempts to provide insights for designing better delivery under public food distribution value chains.Department for International Development (DFID

Bilinear littlewood-paley for circle and transference

In this paper we have obtained the boundedness of bilinear Littlewood-Paley operators on the circle group T by using appropriate transference techniques. In particular, bilinear analogue of Carleson's Littlewood-Paley result for all possible indices has been obtained. Also, we prove some bilinear analogues of de Leeuw's results concerning multipliers of Rn

Private Business-Driven Value Chains and Nutrition: Insights from India

Despite rapid economic growth, undernutrition rates in South Asia remain among the highest in the world. It is also seen that both rural and urban populations in developing countries are increasingly dependent on markets for food. This makes examining the potential of different agri‑food models to deliver nutritious foods relevant. This article examines the value chains of two fortified foods manufactured by private sector business in India using the conceptual framework in Maestre, Poole and Henson (2017), to understand their potential to reach economically poor households. We find that both value chains have potential but are unsuccessful in reaching nutritionally vulnerable populations. In both cases, a favourable institutional environment can enable them to have a pro‑nutrition and pro-poor focus. A proactive state role and regulation are called for to provide the necessary institutional environment to ensure that private business-led value chains focus on enhanced intake of nutritious food by low-income households.Department for International Development (DFID

Hopf Semimetals

We construct two-band topological semimetals in four dimensions using the unstable homotopy of maps from the three-torus $T^3$ (Brillouin zone of a 3D crystal) to the two-sphere $S^2$. Dubbed ``Hopf semimetals'', these gapless phases generically host nodal lines, with a surface enclosing such a nodal line in the four-dimensional Brillouin zone carrying a Hopf flux. These semimetals show a unique class of surface states: while some three-dimensional surfaces host gapless Fermi-arc states {\em and} drumhead states, other surfaces have gapless Fermi surfaces. Gapless two-dimensional corner states are also present at the intersection of three-dimensional surfaces.Comment: 5 pages, 4 figures + Supplemental Material (4 pages, 2 figures