I bisogni professionali del territorio e le esigenze formative dei corsisti: La progettazione dei CPIA nel PON

The analysis of the twelve CPIA’s experiences, in the context of the National Operational Plan (PON) “Per la Scuola” 2014–2020, makes it possible to recognize in the training courses for the Rafforzamento delle competenze di base anche legate ad interventi di formazione professionale of the real practices of expansion of the training offer and effective link between CPIA and training agency, company, secondary school and all the subjects of the Territorial Network for Lifelong Learning. The PON has been a useful tool to respond both to the training needs of the students with training interventions that involve the education, training and work services and to the need for effective collaboration between all the institutional subjects and those not involved in the creation of the Network.Il racconto e l’analisi delle esperienze realizzate da dodici CPIA nell’ambito del PON “Per la Scuola” 2014-2020, ha permesso di riconoscere nei percorsi formativi finalizzati al Rafforzamento delle competenze di base anche legate ad interventi di formazione professionale delle vere e proprie pratiche di ampliamento dell’offerta formativa e di raccordo efficace tra CPIA ed enti della formazione, imprese, istituti di II grado e tutti i soggetti della Rete Territoriale per l’Apprendimento Permanente. Il PON ha rappresentato uno strumento utile per rispondere da una parte, al fabbisogno formativo dei corsisti con interventi formativi che prevedano il coinvolgimento dei servizi di istruzione, formazione e lavoro e dall’altra al fabbisogno di una collaborazione fattiva tra tutti i soggetti istituzionali e non coinvolti nella realizzazione della Rete

A Credibility Assessment Plan for an In Silico Model that Predicts the Dose–Response Relationship of New Tuberculosis Treatments

Tuberculosis is one of the leading causes of death in several developing countries and a public health emergency of international concern. In Silico Trials can be used to support innovation in the context of drug development reducing the duration and the cost of the clinical experimentations, a particularly desirable goal for diseases such as tuberculosis. The agent-based Universal Immune System Simulator was used to develop an In Silico Trials environment that can predict the dose–response of new therapeutic vaccines against pulmonary tuberculosis, supporting the optimal design of clinical trials. But before such in silico methodology can be used in the evaluation of new treatments, it is mandatory to assess the credibility of this predictive model. This study presents a risk-informed credibility assessment plan inspired by the ASME V&V 40‐2018 technical standard. Based on the selected context of use and regulatory impact of the technology, a detailed risk analysis is described together with the definition of all the verification and validation activities and related acceptability criteria. The work provides an example of the first steps required for the regulatory evaluation of an agent-based model used in the context of drug development

Gene expression and pathway bioinformatics analysis detect a potential predictive value of MAP3K8 in thyroid cancer progression

Thyroid cancer is the commonest endocrine malignancy. Mutation in the BRAF serine/threonine kinase is the most frequent genetic alteration in thyroid cancer. Target therapy for advanced and poorly differentiated thyroid carcinomas include BRAF pathway inhibitors. Here, we evaluated the role of MAP3K8 expression as a potential driver of resistance to BRAF inhibition in thyroid cancer. By analyzing Gene Expression Omnibus data repository, across all thyroid cancer histotypes, we found that MAP3K8 is up-regulated in poorly differentiated thyroid carcinomas and its expression is related to a stem cell like phenotype and a poorer prognosis and survival. Taken together these data unravel a novel mechanism for thyroid cancer progression and chemo-resistance and confirm previous results obtained in cultured thyroid cancer stem cellsComment: 5 page

Liposome-Mediated Inhibition of Inflammation by Hydroxycitrate

Hydroxycitrate (HCA), a main organic acid component of the fruit rind of Garcinia cambogia, is a natural citrate analog that can inhibit the ATP citrate lyase (ACLY) enzyme with a consequent reduction of inflammatory mediators (i.e., nitric oxide (NO), reactive oxygen species (ROS), and prostaglandin E2 (PGE2)) levels. Therefore, HCA has been proposed as a novel means to prevent, treat, and ameliorate conditions involving inflammation. However, HCA presents a low membrane permeability, and a large quantity is required to have a biological effect. To overcome this problem, HCA was formulated in liposomes in this work, and the enhancement of HCA cell availability along with the reduction in the amount required to downregulate NO, ROS, and PGE2 in macrophages were assessed. The liposomes were small in size (~60 nm), monodispersed, negatively charged (−50 mV), and stable on storage. The in vitro results showed that the liposomal encapsulation increased by approximately 4 times the intracellular accumulation of HCA in macrophages, and reduced by 10 times the amount of HCA required to abolish LPS-induced NO, ROS, and PGE2 increase. This suggests that liposomal HCA can be exploited to target the citrate pathway involved in inflammatory processes

OUTCOME DI SVILUPPO A BREVE TERMINE IN SOGGETTI SOTTOPOSTI A TRATTAMENTO IPOTERMICO

Trattamento ipotermico, asfissi