3,611 research outputs found
Continued fraction representation of the Coulomb Green's operator and unified description of bound, resonant and scattering states
If a quantum mechanical Hamiltonian has an infinite symmetric tridiagonal
(Jacobi) matrix form in some discrete Hilbert-space basis representation, then
its Green's operator can be constructed in terms of a continued fraction. As an
illustrative example we discuss the Coulomb Green's operator in
Coulomb-Sturmian basis representation. Based on this representation, a quantum
mechanical approximation method for solving Lippmann-Schwinger integral
equations can be established, which is equally applicable for bound-, resonant-
and scattering-state problems with free and Coulombic asymptotics as well. The
performance of this technique is illustrated with a detailed investigation of a
nuclear potential describing the interaction of two particles.Comment: 7 pages, 4 ps figures, revised versio
Three-potential formalism for the three-body scattering problem with attractive Coulomb interactions
A three-body scattering process in the presence of Coulomb interaction can be
decomposed formally into a two-body single channel, a two-body multichannel and
a genuine three-body scattering. The corresponding integral equations are
coupled Lippmann-Schwinger and Faddeev-Merkuriev integral equations. We solve
them by applying the Coulomb-Sturmian separable expansion method. We present
elastic scattering and reaction cross sections of the system both below
and above the threshold. We found excellent agreements with previous
calculations in most cases.Comment: 12 pages, 3 figure
Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching
Natural and synthetic nucleic acids are known to exert immunomodulatory properties. Notably, nucleic acids are known to modulate immune function via several different pathways and various cell types, necessitating a complex interpretation of their effects. In this study we set out to compare the effects of a CpG motif containing oligodeoxynucleotide (ODN) with those of a control and an inhibitory non-CpG ODN during cognate B cell-T cell interactions. We employed an antigen presentation system using splenocytes from TCR transgenic DO11.10 mice and the ovalbumin peptide recognized by the TCR as model antigen. We followed early activation events by measuring CD69 expression, late activation by MHC class II expression, cell division and antibody production of switched, and nonswitched isotypes. We found that both of the tested non-CpG ODN exerted significant immunomodulatory effects on early T cell and on late B cell activation events. Importantly, a synergism between non-CpG effects and T cell help acting on B cells was observed, resulting in enhanced IgG production following cognate T cell-B cell interactions. We propose that non-CpG ODN may perform as better adjuvants when a strong antigen-independent immune activation, elicited by CpG ODNs, is undesirable
Markers of inflammation and bone remodelling associated with improvement in clinical response measures in psoriatic arthritis patients treated with golimumab
<p>Objective To determine serum biomarker associations with clinical response to golimumab treatment in patients with psoriatic arthritis (PsA).</p>
<p>Methods GO–REVEAL was a randomised, placebo-controlled study of golimumab in patients with active PsA. Samples were collected from 100 patients at baseline, week 4 and week 14, and analysed for serum-based biomarkers and protein profiling (total 92 markers); data were correlated with clinical measures at week 14.</p>
<p>Results Serum levels of a subset of proteins (apolipoprotein C III, ENRAGE, IL-16, myeloperoxidase, vascular endothelial growth factor, pyridinoline, matrix metalloproteinase 3, C-reactive protein (CRP), carcinoembryonic antigen, intercellular adhesion molecule 1 and macrophage inflammatory protein 1α) at baseline or week 4 were strongly associated with American College of Rheumatology 20% improvement (ACR20) response and/or disease activity score in 28 joints (DAS28) at week 14. A smaller subset of proteins was significantly associated with a 75% improvement in the psoriasis area and severity index score (PASI75) at week 14, (adiponectin, apolipoprotein CIII, serum glutamic oxaloacetic transaminase, and tumour necrosis factor α). Subsets of proteins were identified as potentially predictive of clinical response for each of the clinical measures, and the power of these biomarker panels to predict clinical response to golimumab treatment was stronger than for CRP alone.</p>
<p>Conclusions This analysis provides insight into several panels of markers that may have utility in identifying PsA patients likely to have ACR20, DAS28, or PASI75 responses following golimumab treatment.</p>
Renormalization Group Flow Equations and the Phase Transition in O(N)-models
We derive and solve flow equations for a general O(N)-symmetric effective
potential including wavefunction renormalization corrections combined with a
heat-kernel regularization. We investigate the model at finite temperature and
study the nature of the phase transition in detail. Beta functions, fixed
points and critical exponents \beta, \nu, \delta and \eta for various N are
independently calculated which allow for a verification of universal scaling
relations.Comment: 34 pages, 3 tables, 11 postscript figures, LaTe
dUTPase based switch controls transfer of virulence genes in order to preserve integrity of the transferred mobile genetic elements
dUTPases ubiquitously regulate cellular dUTP levels to preserve
genome integrity. Recently, several other cellular processes were
reported to be controlled by dUTPases including the horizontal
transfer of Staphylococcus aureus pathogenicity islands (SaPI).
SaPIs are mobil genetic elements that encode virulence enhancing
factors e.g. toxins. Here, phage dUTPases were proposed to
counteract the repressor protein (Stl) and promote SaPI excision
and transfer. A G protein-like mechanism was proposed which is
unexpected in light of the kinetic mechanism of dUTPase.
Here we investigate the molecular mechanism of SaPI transfer
regulation, using numerous dUTPase variants and a wide range
of in vitro methods (steady-state and transient kinetics, VIS and
fluorescence spectroscopy, EMSA, quartz crystal microbalance,
X-ray crystallography).
Our results unambiguously show that Stl inhibits the enzymatic
activity of dUTPase in the nM concentration range and
dUTP strongly inhibits the dUTPase: Stl complexation. These
results identify Stl as a highly potent dUTPase inhibitor protein
and disprove the G protein-like mechanism. Importantly, our
results clearly show that the dUTPase:dUTP complex is inaccessible
to the Stl repressor. Unlike in small GTPases, hydrolysis of
the substrate nucleoside triphosphate (dUTP in this case) is
required prior to the interaction with the partner (Stl repressor in
this case). We propose that dUTPase can efficiently interact with
Stl and induce SaPI excision only if the cellular dUTP level is low (i.e. when dUTPase resides mainly in the apo enzyme form)
while high dUTP levels would inhibit SaPI transfer. This mechanism
may serve the preservation of the integrity of the transferred
SaPI genes and links the well-known metabolic role of
dUTPases to their newly revealed regulatory function in spread
of virulence factors
Is Overtourism overused? Understanding the impact of tourism in a city context
In less than two years, the concept of overtourism has come to prominence as one of the most discussed issues with regards to tourism in popular media and, increasingly, academia. In spite of its popularity, the term is still not clearly delineated and remains open to multiple interpretations. The current paper aims to provide more clarity with regard to what overtourism entails by placing the concept in a historical context and presenting results from a qualitative investigation among 80 stakeholders in 13 European cities. Results highlight that overtourism describes an issue that is multidimensional and complex. Not only are the issues caused by tourism and nontourism stakeholders, but they should also be viewed in the context of wider societal and city developments. The article concludes by arguing that while the debate on overtourism has drawn attention again to the old problem of managing negative tourism impacts, it is not well conceptualized. Seven overtourism myths are identified that may inhibit a well-rounded understanding of the concept. To further a contextualized understanding of overtourism, the paper calls for researchers from other disciplines to engage with the topic to come to new insights
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