Role of antiviral therapy in the natural history of hepatitis B virus-related chronic liver disease

Hepatitis B virus (HBV) infection is a dynamic state of interactions among HBV, hepatocytes, and the host immune system. Natural history studies of chronic hepatitis B (CHB) infection have shown an association between active viral replication and adverse clinical outcomes such as cirrhosis and hepatocellular carcinoma. The goal of therapy for CHB is to improve quality of life and survival by preventing progression of the disease to cirrhosis, decompensation, end-stage liver disease, hepatocellular carcinoma (HCC) and death. This goal can be achieved if HBV replication is suppressed in a sustained manner. The accompanying reduction in histological activity of CHB lessens the risk of cirrhosis and of HCC, particularly in non-cirrhotic patients. However, CHB infection cannot be completely eradicated, due to the persistence of covalently closed circular DNA in the nucleus of infected hepatocytes, which may explain HBV reactivation. Moreover, the integration of the HBV genome into the host genome may favour oncogenesis, development of HCC and may also contribute to HBV reactivation

Effect of the temperature in a mixed culture pilot scale aerobic process for food waste and sewage sludge conversion into polyhydroxyalkanoates

The utilisation of urban organic waste as feedstock for polyhydroxyalkanoates (PHA) production is growing since it allows to solve the main concerns about their disposal and simultaneously to recover added-value products. A pilot scale platform has been designed for this purpose. The VFA-rich fermentation liquid coming from the anaerobic treatment of both source-sorted organic fraction of municipal solid waste (OFMSW) and waste activated sludge (WAS) has been used as substrate for the aerobic process steps: a first sequencing batch reactor (SBR, 100 L) for the selection of a PHA-producing biomass, and a second fed-batch reactor (70 L) for PHA accumulation inside the cells. The SBR was operated at 2.0-4.4 kg COD/(m3 d) as OLR, under dynamic feeding regime (feast-famine) and short hydraulic retention time (HRT; 1 day). The selected biomass was able to accumulate up to 48% g PHA/g VSS. Both steps were performed without temperature (T) control, avoiding additional consumption of energy. In this regard, the applied OLR was tuned based on environmental T and, as a consequence, on biomass kinetic, in order to have a constant selective pressure. The latter was mainly quantified by the PHA storage yield (YP/Sfeast 0.34-0.45 CODP/CODS), which has been recognized as the main parameters affecting the global PHA productivity [1.02-1.82 g PHA/(L d)] of the process

Effectiveness of different strategies to prevent from heat stress in a group of dairy farms located in the Province of Padova

Heat stress during the summer season is an important factor which can impair dairy cows physiology and productivity. A survey was carried out on a sample of 30 dairy farms of the Province of Padova to assess the effectiveness of different strategies for heat stress control. All farms used a fan cooling system but in those were a sprinkler device was also operating an increased milk yield was observed (+5.0%). Cows receiving the diet in two daily distributions (morning and evening) increased DM intake (+9.0%) and milk yield (+15.0%) in comparison to animals fed once a day. No difference, instead, were observed in farms where cows were fed once a day in the morning or in the evening. A positive milk response (+8.1%) was recorded in farms equipped with wide waterers at the exit of the milking parlour

Predictive ability of mid-infrared spectroscopy for major mineral composition and coagulation traits of bovine milk by using the uninformative variable selection algorithm

Milk minerals and coagulation properties are important for both consumers and processors, and they can aid in increasing milk added value. However, large-scale monitoring of these traits is hampered by expensive and time-consuming reference analyses. The objective of the present study was to develop prediction models for major mineral contents (Ca, K, Mg, Na, and P) and milk coagulation properties (MCP: rennet coagulation time, curd-firming time, and curd firmness) using mid-infrared spectroscopy. Individual milk samples (n = 923) of Holstein-Friesian, Brown Swiss, Alpine Grey, and Simmental cows were collected from single-breed herds between January and December 2014. Reference analysis for the determination of both mineral contents and MCP was undertaken with standardized methods. For each milk sample, the mid-infrared spectrum in the range from 900 to 5,000 cm 121 was stored. Prediction models were calibrated using partial least squares regression coupled with a wavenumber selection technique called uninformative variable elimination, to improve model accuracy, and validated both internally and externally. The average reduction of wavenumbers used in partial least squares regression was 80%, which was accompanied by an average increment of 20% of the explained variance in external validation. The proportion of explained variance in external validation was about 70% for P, K, Ca, and Mg, and it was lower (40%) for Na. Milk coagulation properties prediction models explained between 54% (rennet coagulation time) and 56% (curd-firming time) of the total variance in external validation. The ratio of standard deviation of each trait to the respective root mean square error of prediction, which is an indicator of the predictive ability of an equation, suggested that the developed models might be effective for screening and collection of milk minerals and coagulation properties at the population level. Although prediction equations were not accurate enough to be proposed for analytic purposes, mid-infrared spectroscopy predictions could be evaluated as phenotypic information to genetically improve milk minerals and MCP on a large scale

Milk Fatty Acids Predicted by Mid-infrared Spectroscopy in Mixed Dairy Herds

Over the last years, healthy food has gained interest among consumers, especially with regard to the fat content of livestock products which has been associated to the risk of cardiovascular diseases. Individual milk samples (n = 12,624) of 2,977 Holstein-Friesian (HF), Brown Swiss (BS) and Simmental (SI) cows from 39 multibreed herds were analyzed for fat content, protein content, casein content and somatic cell count using mid-infrared spectroscopy (MIRS). Daily milk yield was also recorded. Groups of fatty acids (FA), expressed as percentage of milk fat, were predicted by MIRS: they were saturated (SFA), unsaturated (UFA), monounsaturated (MUFA) and polyunsaturated (PUFA) FA. Data were analyzed with a linear mixed model including the fixed effects of month of sampling, parity, days in milk (DIM), herd, breed, and interactions between parity and breed, and DIM and breed. The random effects were cow nested within breed and residual. Milk of HF cows exhibited the lowest percentage of SFA (70.45%) and the highest of UFA (31.20%), and milk of SI cows was intermediate between that of HF and BS breeds for all groups of FA. The values of groups of FA across DIM were similar for the different breeds. Results from this study indicate that, under similar environmental and management conditions, milk of HF exhibits better FA profile than milk of BS and SI

Milk Fatty Acids Predicted by Mid-infrared Spectroscopy in Mixed Dairy Herds

Over the last years, healthy food has gained interest among consumers, especially with regard to the fat content of livestock products which has been associated to the risk of cardiovascular diseases. Individual milk samples (n = 12,624) of 2,977 Holstein-Friesian (HF), Brown Swiss (BS) and Simmental (SI) cows from 39 multibreed herds were analyzed for fat content, protein content, casein content and somatic cell count using mid-infrared spectroscopy (MIRS). Daily milk yield was also recorded. Groups of fatty acids (FA), expressed as percentage of milk fat, were predicted by MIRS: they were saturated (SFA), unsaturated (UFA), monounsaturated (MUFA) and polyunsaturated (PUFA) FA. Data were analyzed with a linear mixed model including the fixed effects of month of sampling, parity, days in milk (DIM), herd, breed, and interactions between parity and breed, and DIM and breed. The random effects were cow nested within breed and residual. Milk of HF cows exhibited the lowest percentage of SFA (70.45%) and the highest of UFA (31.20%), and milk of SI cows was intermediate between that of HF and BS breeds for all groups of FA. The values of groups of FA across DIM were similar for the different breeds. Results from this study indicate that, under similar environmental and management conditions, milk of HF exhibits better FA profile than milk of BS and SI

Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis.

Cytokinesis culminates in the final separation, or abscission, of the two daughter cells at the end of cell division. Abscission relies on an organelle, the midbody, which forms at the intercellular bridge and is composed of various proteins arranged in a precise stereotypic pattern. The molecular mechanisms controlling midbody organization and function, however, are obscure. Here we show that proper midbody architecture requires cross-regulation between two cell division kinases, Citron kinase (CIT-K) and Aurora B, the kinase component of the chromosomal passenger complex (CPC). CIT-K interacts directly with three CPC components and is required for proper midbody architecture and the orderly arrangement of midbody proteins, including the CPC. In addition, we show that CIT-K promotes Aurora B activity through phosphorylation of the INCENP CPC subunit at the TSS motif. In turn, Aurora B controls CIT-K localization and association with its central spindle partners through phosphorylation of CIT-K's coiled coil domain. Our results identify, for the first time, a cross-regulatory mechanism between two kinases during cytokinesis, which is crucial for establishing the stereotyped organization of midbody proteins.C.M. holds a Cambridge Cancer Centre PhD studentship, which is supported by Cancer Research UK and the MRC. Z.I.B. was supported by a Gwynaeth Pretty PhD studentship and the BlueGnome Molecular Genetics Fund from the Department of Pathology, University of Cambridge, and was also a recipient of a short-term EMBO fellowship and a fellowship from the Cambridge Philosophical Society. G.C. and M.G. are supported by a grant ‘Progetto di Ricerca di Interesse Nazionale’ (PRIN 2012) from the Ministero dell'Istruzione, Università e Ricerca. P.P.D. is the recipient of the Maplethorpe Fellowship from Murray Edwards College, Cambridge, UK.This is the final version of the article. It first appeared from Royal Society Publishing via https://doi.org/10.1098/rsob.16001