Antimicrobial activity of some plant essential oils against Listeria monocytogenes

The antimicrobial activity of 32 plant essential oils commonly used in food industry was examined against four strains of Listeria monocytogenes and one strain of Listeria innocua . Two different procedures were carried out to test the essential oils, a paper disc diffusion method and an inhibition curve. In the former procedure an absolute ethanolic solution (1:5 v/v) of each oil was tested on the plates inoculated with a bacterial concentration of 106 CFU/ml. Five of the 32 essential oils (cinnamon, clove, origanum, pimento, and thyme) showed antibacterial activity. Some of the five oils were also tested at lower concentration (1:50 v/v). The inhibition curve to study antilisteric efficacies of the five oils in a saline solution system was examined. Pimento oil showed marked and rapid activity (generally within 1 h of exposure), whereas clove, origanum, and thyme oils showed a more slow activity. The antilisteric activity of the tested oils seems to be strain dependent. A L. monocytogenes strain was also tested in a food matrix (minced pork meat) against thyme essential oil. Minced pork meat with thyme oil reduced the L. monocytogenes population by ca. 100-fold over the first week of storage

Hydrological and Hydraulic Flood Hazard Modeling in Poorly Gauged Catchments: An Analysis in Northern Italy

Flood hazard is assessed for a watershed with scarce hydrological data in the lower plain of Northern Italy, where the current defense system is inadequate to protect a highly populated urban area located at a river confluence and crossed by numerous bridges. An integrated approach is adopted. Firstly, to overcome the scarcity of data, a regional flood frequency analysis is performed to derive synthetic design hydrographs, with an original approach to obtain the flow reduction curve from recorded water stages. The hydrographs are then imposed as upstream boundary conditions for hydraulic modeling using the fully 2D shallow-water model PARFLOOD with the recently proposed inclusion of bridges. High‐resolution simulations of the potential flooding in the urban center and surrounding areas are, therefore, performed as a novel extensive application of a truly 2D framework for bridge modeling. Moreover, simulated flooded areas and water levels, with and without bridges, are compared to quantify the interference of the crossing structures and to assess the effectiveness of a structural measure for flood hazard reduction, i.e., bridge adaptation. This work shows how the use of an integrated hydrological–hydraulic approach can be useful for infrastructure design and civil protection purposes in a poorly gauged watershed

Experimental and numerical evaluation of the force due to the impact of a dam-break wave on a structure

Flood events caused by the collapse of dams or river levees can have damaging consequences on buildings and infrastructure located in prone areas. Accordingly, a careful prediction of the hydrodynamic load acting on structures is important for flood hazard assessment and potential damage evaluation. However, this represents a challenging task and requires the use of suitable mathematical models. This paper investigates the capability of three different models, i.e. a 2D depth-averaged model, a 3D Eulerian two-phase model, and a 3D Smoothed Particle Hydrodynamics (SPH) model, to estimate the impact load exerted by a dam-break wave on an obstacle. To this purpose, idealised dam-break experiments were carried out by generating a flip-through impact against a rigid squat structure, and measurements of the impact force were obtained directly by using a load cell. The dynamics of the impact event was analyzed and related to the measured load time history. A repeatability analysis was performed due to the great variability typically shown by impact phenomena, and a confidence range was estimated. The comparison between numerical results and experimental data shows the capability of 3D models to reproduce the key features of the flip-through impact. The 2D modelling based on the shallow water approach is not entirely suitable to accurately reproduce the load hydrograph and predict the load peak values; this difficulty increases with the strength of the wave impact. Nevertheless, the error in the peak load estimation is in the order of 10% only, thus the 2D approach may be considered appropriate for practical applications. Moreover, when the shallow water approximation is expected to work well, 2D results are comparable with the experimental data, as well as with the numerical predictions of far more sophisticated and computationally demanding 3D solvers. All the numerical models overestimate the falling limb of the load hydrograph after the impact. The SPH model ensures good evaluation of the long-time load impulse. The 2D shallow water solver and the 3D Eulerian model are less accurate in predicting the load impulse but provide similar results. A sensitivity analysis with respect to the model parameters allows to assess model uncertainty

Distribution of epidemic clonal genetic markers among Listeria monocytogenes 4b isolates.

Recent genome sequencing of isolates of Listeria monocytogenes serotype 4b implicated in some major outbreaks of foodborne listeriosis has revealed unique genetic markers in these isolates. The isolates were grouped into two distinct epidemic clones, ECI and ECII. In the present study, selected ECI- and ECII-specific genetic markers were detected in 16 and 15 of 89 L. monocytogenes 4b isolates, respectively. The ECI markers were found in 6 of 34 clinical isolates, 9 of 50 food isolates, and 1 of 5 environmental isolates, and the ECII markers were detected in 7 of 34 clinical isolates, 7 of 50 food isolates, and 1 of 5 environmental isolates. Hence, of the isolates with the epidemic clonal genetic markers, 38% (13 of 34) were of clinical origin, 32% (16 of 50) were of food origin, and 40% (2 of 5) were of environmental origin. The predominance of the epidemic clonal markers among the clinical and environmental isolates supports the hypothesis that these markers are correlated with the pathogenic potential of strains and with their environmental persistence. Several isolates had only one epidemic clonal marker, either the ECI-specific marker 133 or the ECII-specific marker 4bSF18. Pulsed-field gel electrophoresis analysis revealed higher genomic diversity among the strains with ECII-like characteristics than among those strains carrying the ECI-specific genetic markers

Survival of Listeria monocytogenes in uncooked Italian dry sausage (salami).

This study was undertaken to supplement existing information on the survival of Listeria monocytogenes in Italian salami. The fact that Italian salami is frequently consumed by a large number of people poses some serious health implications. Some raw materials have been found to be microbiologically contaminated, for their production occurs without any thermic treatment, and these are in circulation throughout Italy all year round. We selected the product for its microbiological, technological, and commercial characteristics. We analyzed 1,020 samples taken during the autumn and winter 2002 and spring and summer 2003 periods and immediately before selling. The samples were collected from 17 plants with an annual production of between 1 and 2,000 metric tons and with a distribution of products in over 80% of Italy in geographic terms. To detect and enumerate L. monocytogenes, we followed International Organization for Standardization (ISO) 11290 part 1 and 2: 1996 (modified using chromogenic medium Agar Listeria according to Ottarviani and Agosti [ALOA]). L. monocytogenes was found in 22.7% of samples, but the contamination level was less than 10 CFU/g. Contamination prevalence ranged from 1.6 to 58.3% and was lower than 10% in 5 of the 17 plants checked. The most frequently isolated serotypes were 1/2c, 1/2a, 1/2b, and 4b. Additional studies are necessary to establish if the exposure to a small number of L. monocytogenes cells through the consumption of salami represents a significant health risk and, in light of the future introduction of the SANCO/4198/2001 revision 21 "Commission Regulation on Microbiological Criteria for Foodstuffs," is a necessary investigation

Clostridium botulinum spores and toxin in mascarpone cheese and other milk products

A total of 1,017 mascarpone cheese samples, collected at retail, were analyzed for Clostridium botulinum spores and toxin, aerobic mesophilic spore counts, as well as pH, a(w) (water activity), and Eh (oxidation-reduction potential). In addition 260 samples from other dairy products were also analyzed for spores and botulinum toxin. Experiments were carried out on naturally and artificially contaminated mascarpone to investigate the influence of different temperature conditions on toxin production by C. botulinum. Three hundred and thirty-one samples (32.5%) of mascarpone were positive for botulinal spores, and 7 (0.8%) of the 878 samples produced at the plant involved in an outbreak of foodborne botulism also contained toxin type A. The chemical-physical parameters (pH, a(w), Eh) of all samples were compatible with C. botulinum growth and toxinogenesis. Of the other milk products, 2.7% were positive for C. botulinum spores. Growth and toxin formation occurred in naturally and experimentally contaminated mascarpone samples after 3 and 4 days of incubation at 28 degrees C, respectively

Middle Palaeolithic technical behaviour: Material import-export and Levallois production at the SU 13 of Oscurusciuto rock shelter, Southern Italy

The Oscurusciuto rock shelter, located in southern Italy (Puglia), has yielded a long Middle Palaeolithic stratigraphy rich in lithic assemblages, fireplaces and faunal remains, attesting Neanderthal occupation during the MIS 3. This paper is focused on the stratigraphic unit 13, consisting of a sandy compact deposit mixed with pyroclastic sediment above a thick level of tephra-US 14, identified as Mt. Epomeo green tuff (dated Ar/Ar ~ 55 ka).Level 13 represents the first stable human occupation after the deposition of tephra. Our goal was to examine the lithic assemblage of this stratigraphic unit by means of an interdisciplinary approach (technology, RMU, refitting program) in order to identify the economic behaviour and technical strategies of Neanderthals occupying the stratigraphic unit 13 of Oscurusciuto.The technical strategies applied indicate fragmentation of the reduction processes, as well as probable events of importation and exportation of objects. The lithic material were introduced at different stages of manufacturing. Pieces were introduced in the form of rough objects (pebbles), as well as semi-finished items, and as finished tools. This fragmentation of the chaîne opératoire also demonstrate the palimpsest nature of the level which is made up of different events happening one after another.The main concept of debitage was Levallois, generally realized on local jasper and siliceous limestone pebbles or cortical flakes. Jasper and siliceous limestone flakes, backed flakes and convergent flakes were the technological objectives of the debitage. A marginal volumetric debitage aimed at producing bladelets was also attested.

Middle Palaeolithic lithic tools: Techno-functional and use-wear analysis of target objects from SU 13 at the Oscurusciuto rock shelter, Southern Italy

The Oscurusciuto rock shelter (Ginosa, Puglia, southern Italy) is a Middle Palaeolithic site characterized by a significant stratigraphy made up by several anthropic levels. The stratigraphic unit 13, consisting of a sandy compact deposit mixed with pyroclastic sediment, is a short palimpsest situated on a layer of tephra, identified as Mt. Epomeo green tuff (dated Ar/Ar ~ 55 ka BP). From a technological point of view, the aims of the production were backed flakes, convergent flakes, and other flakes obtained by means of a Levallois debitage, plus (less represented) bladelets produced by an additional volumetric reduction system. Our aim in this research was to examine a selection of the above-mentioned target objects produced by debitage in order to understand the manufacture and life-cycle of each single tool from a dynamic perspective. We integrated techno-functional and use-wear analyses: the first was implemented to globally comprehend each tool, identifying each single techno-functional unity (prehensile and transformative portions), whereas the second revealed the way in which these tools had been used, proceeding to identify the activity involved (e.g., piercing, cutting and/or scraping), and the type of material (vegetable/animal, soft/hard) on which these activities had been carried out. The combined use of these two approaches allows us to ascertain the intention of the prehistoric craftsmen, the gestures and procedures involved in making the tools, and the way they had been used. From one single object we are thus able to reconstruct a series of complex behaviours, encompassing the creation, the life-cycle and finally the ‘death’ or repurposing of the tool in question

Tissue distribution and acute toxicity of silver after single intravenous administration in mice: nano-specific and size-dependent effects

Background: Silver nanoparticles (AgNPs) are an important class of nanomaterials used as antimicrobial agents for a wide range of medical and industrial applications. However toxicity of AgNPs and impact of their physicochemical characteristics in in vivo models still need to be comprehensively characterized. The aim of this study was to investigate the effect of size and coating on tissue distribution and toxicity of AgNPs after intravenous administration in mice, and compare the results with those obtained after silver acetate administration. Methods: Male CD-1(ICR) mice were intravenously injected with AgNPs of different sizes (10 nm, 40 nm, 100 nm), citrate-or polyvinylpyrrolidone-coated, at a single dose of 10 mg/kg bw. An equivalent dose of silver ions was administered as silver acetate. Mice were euthanized 24 h after the treatment, and silver quantification by ICP-MS and histopathology were performed on spleen, liver, lungs, kidneys, brain, and blood. Results: For all particle sizes, regardless of their coating, the highest silver concentrations were found in the spleen and liver, followed by lung, kidney, and brain. Silver concentrations were significantly higher in the spleen, lung, kidney, brain, and blood of mice treated with 10 nm AgNPs than those treated with larger particles. Relevant toxic effects (midzonal hepatocellular necrosis, gall bladder hemorrhage) were found in mice treated with 10 nm AgNPs, while in mice treated with 40 nm and 100 nm AgNPs lesions were milder or negligible, respectively. In mice treated with silver acetate, silver concentrations were significantly lower in the spleen and lung, and higher in the kidney than in mice treated with 10 nm AgNPs, and a different target organ of toxicity was identified (kidney). Conclusions: Administration of the smallest (10 nm) nanoparticles resulted in enhanced silver tissue distribution and overt hepatobiliary toxicity compared to larger ones (40 and 100 nm), while coating had no relevant impact. Distinct patterns of tissue distribution and toxicity were observed after silver acetate administration. It is concluded that if AgNPs become systemically available, they behave differently from ionic silver, exerting distinct and size-dependent effects, strictly related to the nanoparticulate form