Effects of Essential Hypertension on coronary Microcirculation: Focus on a Population of Hypertensives Affected by Microvascular Angina

A correlation between essential hypertension and the establishmentof myocardial ischemia is nowadays universally accepted. Coronary atherosclerosis is deemed to be the most important process through which the capability of coronary district to supply a blood flow consistent with myocardial needs can be impaired, until the onset of an anginal syndrome. In this study, we verified whether hypertensives’ coronaries, seen by performing an angiographic study, are properly definable as normal, even in presence of an overt exertional angina, or if they should rather be barely defined as “macroscopically unharmed”, through the clues of a microvascular alteratio

A suspected case of hunting accident. Case report

Nowadays, the use of molecular biology in forensics has made it possible to identify human victim and sometimes even the circumstances under which the death occurred through. In our case, a corpse of a 50-years old man with a gunshot wound was found in the woods. The suspected murderer declared that it had been a hunting accident while he shot a wild boar. During the autopsy, a bullet (Borra-bullet Gualandi, 32 gr) was found in the abdomen of the victim. The authors investigated the presence of boar and victim blood both on the bullet, in order to substantiate the thesis of the hunting accident. Laboratory investigations underscored the presence of human cellular material on the bullet, which corresponds to profile of the victim and human and porcine ribosomal DNA. Through this investigation, the authors showed that it was a hunting accident, because the bullet passed through the body of the boar before hitting the victim

Analysis of Diagnostic Images of Artworks and Feature Extraction: Design of a Methodology

none6noDigital images represent the primary tool for diagnostics and documentation of the state of preservation of artifacts. Today the interpretive filters that allow one to characterize information and communicate it are extremely subjective. Our research goal is to study a quantitative analysis methodology to facilitate and semi-automate the recognition and polygonization of areas corresponding to the characteristics searched. To this end, several algorithms have been tested that allow for separating the characteristics and creating binary masks to be statistically analyzed and polygonized. Since our methodology aims to offer a conservator-restorer model to obtain useful graphic documentation in a short time that is usable for design and statistical purposes, this process has been implemented in a single Geographic Information Systems (GIS) application.openAmura, Annamaria; Aldini, Alessandro; Pagnotta, Stefano; Salerno, Emanuele; Tonazzini, Anna; Triolo, PaoloAmura, Annamaria; Aldini, Alessandro; Pagnotta, Stefano; Salerno, Emanuele; Tonazzini, Anna; Triolo, Paol

Investigating submerged morphologies by means of the low-budget “GeoDive” method (high resolution for detailed 3D reconstruction and related measurements)

Geophysical methods allow to collect geological data on lake and sea bottoms and characterize large areas, even at high depths, but with high costs. Moreover, the most widespread acquisition methods for morpho-bathymetric survey and the related instruments used are almost always ship-, ROV- or AUV-based and consequently they require high budgets. It is known that shallow waters can represent a limit for certain vessels and techniques, preventing the acquisition in the shoreface zone. To overcome the limits, i.e. to survey with high accuracy nearshore shallow waters with a low budget, we tested and tuned the “GeoDive” method that allowed us to survey two test sites, featured by the presence of “block fields” (i.e., accumulations of huge blocks and boulders of gravitational origin) under shallow waters. The “GeoDive” method allowed us to map the submerged morphologies and to acquire high-resolution optical images for further photogrammetric processing. The latter was fundamental to obtain 3D high-resolution models, also with conditions of low visibility. An Action Sport Cam with high definition resolution has been used for video acquisition, in addition to the equipment used during scientific diving. By coupling the processing of underwater-acquired data with the direct surveys performed by underwater SCUBA operators, it was possible to perform some morphological and sedimentological measurements and observations on the experimental targets, with the help of suitable markers

Slow motion and metastability for a non local evolution equation

In this paper we consider a non local evolution mean field equation proving the existence of an invariant, unstable, one dimensional manifold connecting the critical droplet with the stable and the metastable phases. We prove that the points on the manifold are droplets longer or shorter than the critical one, and that their motion is very slow in agreement with the theory of metastable patterns

Interaction of a Long Alkyl Chain Protic Ionic Liquid and Water

A combined experimental/theoretical approach has been used to investigate the role of water in modifying the microscopic interactions characterizing the optical response of butyl-ammonium nitrate (BAN) water solutions. Raman spectra, dominated by the signal from the protic ionic liquid, were collected as a function of the water content, and the corresponding spatial organization of the ionic couples, as well as their local arrangement with water molecules, was studied exploiting classical molecular dynamics calculations. High quality spectroscopic data, combined with a careful analysis, revealed that water affects the vibrational spectrum BAN in solution: as the water concentration is increased, peaks assigned to stretching modes show a frequency hardening together with a shape narrowing, whereas the opposite behavior is observed for peaks assigned to bending modes. Calculation results clearly show a nanometric spatial organization of the ionic couples that is not destroyed on increasing the water content at least within an intermediate range. Our combined results show indeed that small water concentrations even increase the local order. Water molecules are located among ionic couples and are closer to the anion than the cation, as confirmed by the computation of the number of H-bonds which is greater for water-anion than for water-cation. The whole results set thus clarifies the microscopic scenario of the BAN-water interaction and underlines the main role of the extended hydrogen bond network among water molecules and nitrate anions.Comment: 27 pages, 10 figure

Molecular Dynamics Simulation of Vascular Network Formation

Endothelial cells are responsible for the formation of the capillary blood vessel network. We describe a system of endothelial cells by means of two-dimensional molecular dynamics simulations of point-like particles. Cells' motion is governed by the gradient of the concentration of a chemical substance that they produce (chemotaxis). The typical time of degradation of the chemical substance introduces a characteristic length in the system. We show that point-like model cells form network resembling structures tuned by this characteristic length, before collapsing altogether. Successively, we improve the non-realistic point-like model cells by introducing an isotropic strong repulsive force between them and a velocity dependent force mimicking the observed peculiarity of endothelial cells to preserve the direction of their motion (persistence). This more realistic model does not show a clear network formation. We ascribe this partial fault in reproducing the experiments to the static geometry of our model cells that, in reality, change their shapes by elongating toward neighboring cells.Comment: 10 pages, 3 figures, 2 of which composite with 8 pictures each. Accepted on J.Stat.Mech. (2009). Appeared at the poster session of StatPhys23, Genoa, Italy, July 13 (2007

Doctor of Philosophy

dissertationParalysis can be ameliorated through functional electrical stimulation (FES) of the intact peripheral nerves. The Utah Slanted Electrode Array (USEA) can improve FES systems by providing selective access to many independent motor unit populations.This dissertation includes three studies that expand the role of USEAs in FES applications. The fi rst study leverages the selectivity of the USEA to independently activate the hamstring muscles. Because the di fferent biarticular hamstring muscles can either ex or extend the limb (at the knee or hip), the ability to selectively activate each one independently is required to evoke functional movements such as stance and gait. USEAs implanted in the muscular branch of the sciatic nerve were able to selectively activate each muscle of the hamstring group. Activation of these muscles was graded with increasing stimulus strength, and provided ample dynamic range to allow for fine control of muscle force. The second study demonstrates the ability of the USEA to selectively block neural activity. Upper motor neuron damage can cause hyperre exia and spasticity as well as paralysis. By delivering high-frequency sinusoids through electrodes of the USEA, ber subsets in a nerve were blocked while allowing the remainder of the nerve to function normally. Sinusoids delivered through different electrodes allowed for deactivation of di fferent muscles. The ability to selectively interrupt activity in fiber subpopulations within a nerve will provide new therapeutic options for the positive symptoms of upper motor neuron damage. The fi nal study addresses the practical difficulty of choosing the appropriate stimulus parameters to evoke functional movements. In a USEA-based FES system, the electrodes and stimulus parameters that evoke the desired responses must be identifi ed empirically. USEAs were implanted into three diff erent hind limb nerves, and the response evoked by each electrode was measured noninvasively using 3-D endpoint force. Each electrode was classifi ed as evoking limb flexion or limb extension, and a range of stimulus intensities was identifi ed that evoked a graded force response. Excitation overlap between selected electrode pairs was quantifi ed using the refractory technique. This method will allow for electrode and stimulus parameter selection for use in an FES system using minimal, noninvasive instrumentation

Vaccination against SARS-CoV-2 in pregnancy during the Omicron wave: the prospective cohort study of the Italian obstetric surveillance system

Objectives: Evidence on the effects of the SARS-CoV-2 Omicron variant on vaccinated and unvaccinated pregnant women is sparse. This study aimed to compare maternal and perinatal outcomes of women infected with SARS-CoV-2 during the Omicron wave in Italy, according to their vaccine protection.Methods: This national prospective cohort study enrolled pregnant women with a positive SARS-CoV-2 nasopharyngeal swab within 7 days of hospital admission between 1 January and 31 May, 2022. Women who received at least one dose of vaccine during pregnancy and those who completed the vaccine cycle with the first booster were considered protected against moderate or severe COVID-19 (MSCD). A multivariable logistic regression model evaluated the association between vaccine protection and disease severity. Maternal age, educational level, citizenship, area of birth, previous comorbidities, and obesity were analysed as potential risk factors. Results: MSCD was rare (41/2147, 1.9%; 95% CI, 1.4-2.6), and the odds of developing it were significantly higher among unprotected women (OR, 2.78; 95% CI, 1.39-5.57). Compared with protected women (n = 1069), the unprotected (n = 1078) were more often younger, with lower educational degrees, and foreigners. A higher probability of MSCD was found among women with previous comorbidities (OR, 2.86; 95% CI, 1.34-6.12) and those born in Asian countries (OR, 3.05; 95% CI, 1.23-7.56). The percentage of preterm birth was higher among women with MSCD compared with milder cases (32.0% [8/25] versus 8.4% [161/1917], p < 0.001) as well as the percentage of caesarean section (52.0% [13/25] versus 31.6% [606/1919], p 0.029). Discussion: Although severe maternal and perinatal outcomes were rare, their prevalence was significantly higher among women without vaccine protection. Vaccination during pregnancy has the potential to protect both the mother and the baby, and it is therefore strongly recommended. Edoardo Corsi Decenti, Clin Microbiol Infect 2023;29:772 (c) 2023 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved