Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles

The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.Ministry of Science and Technological Development of the Republic of Serbia [145073

Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles

The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.Ministry of Science and Technological Development of the Republic of Serbia [145073

New national and regional bryophyte records, 37

International audienc

New national and regional bryophyte records, 36

In the moss flora of Uruguay there are recorder ten species of genus Fissidens (Matteri, 2004). Three of them (F. macrobryoides, F. prionocheilos and F. vitreo-limbatus) are species incertae because the type material for the names not was located (Purssel, 2007). As part of project "Studies on Bryophytes in the Cone Sur (Systematic and Phylogeny)" some specimens recently collected in Uruguay were determinate as Fissidens asplenioides a no previously species recorded in this country. The presence of F. asplenioides in Uruguay complete the distribution range of the species in the cone Sur (is present in Argentina, Bolivia, Brazil, Chile and Paraguay). However, within the Neotropical region there are few records of F. asplenoides in the phytogeographic province of Pampa. In Uruguay, where other bryophytes were recently recorded (Ellis et al., 2011, 2012a, b), F. asplenioides was collected in the river on semi-submerged rock.Fil: Ellis, L. T.. Natural History Museum; Reino UnidoFil: Bakalin, V. A.. Botanical Garden-Institute; Rusia. Institute of Biology and Soil Science; RusiaFil: Baisheva, E.. Scientific Centre of Russian Academy of Sciences; RusiaFil: Bednarek Ochyra, H.. Institute of Botany; PoloniaFil: Ochyra, R.. Institute of Botany; PoloniaFil: Borovichev, E. A.. Polar-Alpine Botanical Garden-Institute; RusiaFil: Choi, S. S.. Chonbuk National University; Corea del SurFil: Sun, B. Y.. Chonbuk National University; Corea del SurFil: Erzberger, P.. No especifica;Fil: Fedosov, V. E.. M.V. Lomonosov Moscow State University; RusiaFil: Garilleti, R.. Universidad de Valencia; EspañaFil: Albertos. B.. Universidad de Valencia; EspañaFil: Górski, P.. Poznań University of Life Sciences; PoloniaFil: Hájková, P.. Masaryk University; República Checa. Institute of Botany; República ChecaFil: Hodgetts, N. G.. No especifica;Fil: Ignatov, M.. Main Botanical Garden; RusiaFil: Koczur. A.. Institute of Nature Conservation; PoloniaFil: Kurbatova, L. E.. Komarov Botanical Institute; RusiaFil: Lebouvier, M.. Universite de Rennes I; FranciaFil: Mezăka, A.. University of Latvia; LetoniaFil: Miravet, J.. Jardí Botànic Marimurtra; EspañaFil: Pawlikowski, P.. Uniwersytet Warszawski; ArgentinaFil: Porley, R. D.. No especifica;Fil: Rosselló, J. A.. Jardí Botànic Marimurtra; España. Universidad de Valencia; EspañaFil: Sabovljević, M. S.. University of Belgrade; SerbiaFil: Pantović, J.. University of Belgrade; SerbiaFil: Sabovljević, A.. University of Belgrade; SerbiaFil: Schröder, W.. No especifica;Fil: Ştefănut, S.. Institute of Biology of Romanian Academy; RumaniaFil: Suarez, Guillermo Martin. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; ArgentinaFil: Schiavone, M.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; ArgentinaFil: Yayintas, Ö. T.. Çanakkale Onsekiz Mart University; TurquíaFil: Váňa, J.. Charles University; República Chec