Association between oxidative stress and melanoma progression

Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level

Serum levels of interleukin 27 and oxidative stress parameters in patients with primary skin melanoma

Uvod: Melanom nastaje usled maligne transformacije melanocita i dominantno se javlja u koži, ali se može razviti i u sluznici, oku i u bilo kojem tkivu koje sadrži melanocite. Predstavlja najređi oblik malignih tumora kože, ali i najagresivniji jer je odgovoran za 60% smrtnih ishoda povezanih sa malignim tumorima kože. Glavni ciljevi ove studije bili su ispitati korelaciju serumskih vrednosti IL27 i vrednosti parametara oksidativnog stresa (tSOD, MnSOD, CAT, MDA i O2•–) sa kliničko-patološkim karakteristikama melanoma, kao i ispitati međusobnu povezanost koncentracija IL27 i vrednosti parametara oksidativnog stresa. Pacijenti i metode: U studiju je bilo uključeno 72 ispitanika sa dijagnozom melanoma, prosečne starosti oko 50 godina i 30 zdravih ispitanika. Koncentracija IL27 je određivana metodom ELISA testa, dok su parametri oksidativnog stresa određivani spektrofotometrijskim metodama. Rezultati: Rezultati ovog istraživanja pokazuju da su pacijenti sa melanom imali značajno povećanu produkciju O2•– i nivo markera oksidativnog stresa tj. MDA u odnosu na zdrave osobe, kao i izmenjen mehanizam antioksidativne zaštite (aktivnosti tSOD i MnSOD se nisu razlikovale između kontrolne grupe i obolelih od melanoma, dok je aktivnost SAT bila značajno veća kod obolelih od melanoma). Takođe, pokazano je da koncentracija IL27 značajno veća u serumu zdravih osoba u odnosu na obolele od melanoma. Pokazano je da je interleukin 27 dobar marker bolesti, dok su: O2•-, MDA i CAT osrednje dobri markeri bolesti. Takođe, pokazana je negativna korelacija između sledećih parametara: IL27 i CAT, IL27 i tSOD, IL27 i MnSOD. Zaključak: Dobijeni rezultati ukazuju na moguću protektivnu ulogu IL27 u bolesnika sa melanomom, što može biti korisno za razumevanje efekata IL27 i ukazati na mogućnost upotrebe ovog citokina u imunoterapiji. Praćenje parametra oksidativnog stresa može biti korisno u proceni stepena razvoja oksidativnog stresa i statusa antioksidativne odbrane u funkciji progresije melanoma.Introduction: Melanoma is caused by malignant transformation of melanocytes and predominantly occurs in the skin, but can also develop in the mucous membrane, in the eye and in any tissue containing melanocytes. It is the rarest form of malignant skin tumors, but also the most aggressive because it is responsible for 60% of deaths associated with malignant skin tumors. The main objectives of this study were to examine the correlation of serum IL27 values and the values of oxidative stress parameters (tSOD, MnSOD, CAT, MDA and O2•-) with clinical-pathological characteristics of melanoma, as well as to examine the interconnectedness of IL27 concentrations and the values of oxidative stress parameters. Patients and methods: The study included 72 subjects with a diagnosis of melanoma, an average age of 50 years and 30 healthy subjects. The IL27 concentration was determined by the ELISA test method, while the parameters of oxidative stress were determined by spectrophotometric methods. Results: The results of this study show that patients with melanoma had a significantly increased production of O2•- and level of MDA (markers of oxidative stress), compared to healthy controls, as well as the modified antioxidant mechanism (the activities of tSOD and MnSOD were not different between control group and melanoma patients, while CAT activity was significantly higher in melanoma patients). It has also been shown that the concentration of IL27 is significantly higher in the serum of healthy individuals compared to melanoma patients. Interleukin 27 has been shown to be a good marker of the disease, while: O2•-, MDA and CAT are the moderately good markers of the disease. Also, it has been shown a negative correlation between the following parameters: IL27 and CAT, IL27 and tSOD, IL27 and MnSOD. Conclusion: The obtained results indicate the possible protective role of IL27 in patients with melanoma, which can be useful for understanding the effects of IL27 and to indicate the possibility of using this cytokine in immunotherapy. Monitoring of the oxidative stress parameters can be useful in assessing the degree of development of oxidative stress and the status of antioxidant defense in the function of melanoma progression

Serum levels of interleukin 27 and oxidative stress parameters in patients with primary skin melanoma

Uvod: Melanom nastaje usled maligne transformacije melanocita i dominantno se javlja u koži, ali se može razviti i u sluznici, oku i u bilo kojem tkivu koje sadrži melanocite. Predstavlja najređi oblik malignih tumora kože, ali i najagresivniji jer je odgovoran za 60% smrtnih ishoda povezanih sa malignim tumorima kože. Glavni ciljevi ove studije bili su ispitati korelaciju serumskih vrednosti IL27 i vrednosti parametara oksidativnog stresa (tSOD, MnSOD, CAT, MDA i O2•–) sa kliničko-patološkim karakteristikama melanoma, kao i ispitati međusobnu povezanost koncentracija IL27 i vrednosti parametara oksidativnog stresa. Pacijenti i metode: U studiju je bilo uključeno 72 ispitanika sa dijagnozom melanoma, prosečne starosti oko 50 godina i 30 zdravih ispitanika. Koncentracija IL27 je određivana metodom ELISA testa, dok su parametri oksidativnog stresa određivani spektrofotometrijskim metodama. Rezultati: Rezultati ovog istraživanja pokazuju da su pacijenti sa melanom imali značajno povećanu produkciju O2•– i nivo markera oksidativnog stresa tj. MDA u odnosu na zdrave osobe, kao i izmenjen mehanizam antioksidativne zaštite (aktivnosti tSOD i MnSOD se nisu razlikovale između kontrolne grupe i obolelih od melanoma, dok je aktivnost SAT bila značajno veća kod obolelih od melanoma). Takođe, pokazano je da koncentracija IL27 značajno veća u serumu zdravih osoba u odnosu na obolele od melanoma. Pokazano je da je interleukin 27 dobar marker bolesti, dok su: O2•-, MDA i CAT osrednje dobri markeri bolesti. Takođe, pokazana je negativna korelacija između sledećih parametara: IL27 i CAT, IL27 i tSOD, IL27 i MnSOD. Zaključak: Dobijeni rezultati ukazuju na moguću protektivnu ulogu IL27 u bolesnika sa melanomom, što može biti korisno za razumevanje efekata IL27 i ukazati na mogućnost upotrebe ovog citokina u imunoterapiji. Praćenje parametra oksidativnog stresa može biti korisno u proceni stepena razvoja oksidativnog stresa i statusa antioksidativne odbrane u funkciji progresije melanoma.Introduction: Melanoma is caused by malignant transformation of melanocytes and predominantly occurs in the skin, but can also develop in the mucous membrane, in the eye and in any tissue containing melanocytes. It is the rarest form of malignant skin tumors, but also the most aggressive because it is responsible for 60% of deaths associated with malignant skin tumors. The main objectives of this study were to examine the correlation of serum IL27 values and the values of oxidative stress parameters (tSOD, MnSOD, CAT, MDA and O2•-) with clinical-pathological characteristics of melanoma, as well as to examine the interconnectedness of IL27 concentrations and the values of oxidative stress parameters. Patients and methods: The study included 72 subjects with a diagnosis of melanoma, an average age of 50 years and 30 healthy subjects. The IL27 concentration was determined by the ELISA test method, while the parameters of oxidative stress were determined by spectrophotometric methods. Results: The results of this study show that patients with melanoma had a significantly increased production of O2•- and level of MDA (markers of oxidative stress), compared to healthy controls, as well as the modified antioxidant mechanism (the activities of tSOD and MnSOD were not different between control group and melanoma patients, while CAT activity was significantly higher in melanoma patients). It has also been shown that the concentration of IL27 is significantly higher in the serum of healthy individuals compared to melanoma patients. Interleukin 27 has been shown to be a good marker of the disease, while: O2•-, MDA and CAT are the moderately good markers of the disease. Also, it has been shown a negative correlation between the following parameters: IL27 and CAT, IL27 and tSOD, IL27 and MnSOD. Conclusion: The obtained results indicate the possible protective role of IL27 in patients with melanoma, which can be useful for understanding the effects of IL27 and to indicate the possibility of using this cytokine in immunotherapy. Monitoring of the oxidative stress parameters can be useful in assessing the degree of development of oxidative stress and the status of antioxidant defense in the function of melanoma progression