India Needs Smart Frontiers: An Assessment

In the era of Fourth Industrial Revolution, technology has transformed the ways of doing any business, including warfare. The human element is slowly giving way to machines who would perform major functions including substituting and supplementing rank and file in the Military. Over 22,500 km of India’s frontiers are guarded by various Border Guarding Forces including the Coast Guards to prevent surprise incursions by the adversaries’ State, State-sponsored or Non- State Actors. The Border Guarding Forces act as eyes and ears of the frontiers and can take on basic defensive actions for which colossal manpower is used. For a Military threat, Regular Forces take over the responsibility for the Defense of the Nation. There is a case for optimizing manpower and increasing efficiency by bringing smart systems in delivering constant situational awareness and response solutions. This would also ensure that all stakeholders are networked in real-time. A combination of sensors, electronic and digital platforms, Data and Communication centers, using Terrestrial and Non-Terrestrial means, would build a ‘system of systems for Common Operational Picture at Strategic, Operational and Tactical levels. Data/intelligence analysis and automation would be possible if such systems are ubiquitous and part of the overall National security apparatus. The smart frontiers would soon be an inescapable necessity for the Defense of the nation

India Needs Smart Frontiers: An Assessment

In the era of Fourth Industrial Revolution, technology has transformed the ways of doing any business, including warfare. Thehuman element is slowly giving way to machines who would perform major functions including substituting and supplementing rankand file in the Military. Over 22,500 km of India’s frontiers are guarded by various Border Guarding Forces including the CoastGuards to prevent surprise incursions by the adversaries’ State, State-sponsored or Non- State Actors. The Border Guarding Forces act as eyes and ears of the frontiers and can take on basic defensive actions for which colossal manpower is used. For a Military threat, Regular Forces take over the responsibility for the Defense of the Nation. There is a case for optimizing manpower and increasing efficiency by bringing smart systems in delivering constant situational awareness and response solutions. This would also ensure that all stakeholders are networked in real-time. A combination of sensors, electronic and digital platforms, Data and Communication centers, using Terrestrial and Non-Terrestrial means, would build a ‘system of systems for Common Operational Picture at Strategic, Operational and Tactical levels. Data/intelligence analysis and automation would be possible if such systems are ubiquitous and part of the overall National security apparatus. The smart frontiers would soon be an inescapable necessity for the Defense of the nation

India Needs Smart Frontiers: An Assessment

In the era of Fourth Industrial Revolution, technology has transformed the ways of doing any business, including warfare. Thehuman element is slowly giving way to machines who would perform major functions including substituting and supplementing rankand file in the Military. Over 22,500 km of India’s frontiers are guarded by various Border Guarding Forces including the CoastGuards to prevent surprise incursions by the adversaries’ State, State-sponsored or Non- State Actors. The Border Guarding Forces act as eyes and ears of the frontiers and can take on basic defensive actions for which colossal manpower is used. For a Military threat, Regular Forces take over the responsibility for the Defense of the Nation. There is a case for optimizing manpower and increasing efficiency by bringing smart systems in delivering constant situational awareness and response solutions. This would also ensure that all stakeholders are networked in real-time. A combination of sensors, electronic and digital platforms, Data and Communication centers, using Terrestrial and Non-Terrestrial means, would build a ‘system of systems for Common Operational Picture at Strategic, Operational and Tactical levels. Data/intelligence analysis and automation would be possible if such systems are ubiquitous and part of the overall National security apparatus. The smart frontiers would soon be an inescapable necessity for the Defense of the nation

Development of screening technique for artificial creation of false smut in rice

False smut (Ustilaginoidea virens (Cooke) Takahashi) of rice is an emerging disease and a potential threat to rice growers in different countries due to reduction in yield, quality and production of mycotoxins. Development and cultivation of resistant varieties is desirable approach for its management. Different inoculation techniques viz. spray inoculation, syringe inoculation and dusting method were evaluated for creating artificial epiphytotic conditions in susceptible variety PR116 under natural as well as artificially provided humidity conditions. Spray inoculation method produced more number of smut balls per plant (3.21) and per cent infected panicles (33.44%) followed by syringe inoculation (2.00 and 26.57%, respectively) and dusting method (0.33 and 6.67%, respectively). However, syringe inoculation method produced more spikelet sterility (14.82%) in rice panicles. More disease occurrence was reported in plants provided with artificial humidity after inoculations (51.12% infected panicles) than the plants grown under natural conditions (13.33% infected panicles). Late transplanted crop developed higher disease severity (18.17% infected panicles and 3.34 smut balls/plant) which might be attributed by slight decrease in temperature and increase in relative humidity conditions (up to 88% RH) at time of infection from boot to early flowering stage of the rice crop

Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa)

A study was carried out to find out the favourable culture media for mass multiplication and the effect of carbon sources and duration on chlamydospore germination of Ustilaginoidea virens (Cook.) Tak. Different synthetic and semi synthetic solid, liquid media, substrate media were evaluated for mass multiplication of U. virens. Potato sucrose broth was the best medium for fast mycelia growth (104.80 mg) and sporulation (6.78 × 105 spores/ml), potato dextrose broth also favored mycelial growth, whereas corn meal dextrose broth was not suitable. Out of four solid media tested, maximum colony diameter (68.74 mm) and sporulation (6.86 × 105 spores/ml) was produced by potato sucrose agar followed by potato dextrose agar. Maximum sporulation was produced on substrate media based on barley seeds (5.54 × 105 spores/ml) followed by rice seeds (3.72 × 105 spores/ml) whereas rice husk based media produced minimum sporulation. Higher concentration of sucrose and dextrose at 2 per cent supported maximum germination of spores of U. virens and sucrose was found superior in inducing germination than dextrose. The results of this study help to understand the physiological and biochemical requirements for the growth and development of the pathogen, which could serve as an input in disease management to minimize the effect of false smut disease on rice

Towards an anti-fibrotic therapy for scleroderma: targeting myofibroblast differentiation and recruitment

BACKGROUND: In response to normal tissue injury, fibroblasts migrate into the wound where they synthesize and remodel new extracellular matrix. The fibroblast responsible for this process is called the myofibroblast, which expresses the highly contractile protein alpha-smooth muscle actin (alpha-SMA). In normal tissue repair, the myofibroblast disappears. Conversely, abnormal myofibroblast persistence is a key feature of fibrotic dieases, including scleroderma (systemic sclerosis, SSc). Myofibroblasts can be derived from differentiation of local resident fibroblasts or by recruitment of microvascular pericytes. CLINICAL PROBLEM ADDRESSED: Controlling myofibroblast differentiation and persistence is crucial for developing anti-fibrotic therapies targeting SSc. BASIC SCIENCE ADVANCES: Insights have been recently generated into how the proteins transforming growth factor beta (TGFbeta), endothelin-1 (ET-1), connective tissue growth factor (CCN2/CTGF) and platelet derived growth factor (PDGF) contribute to myofibroblast differentiation and pericyte recruitment in general and to the persistent myofibroblast phenotype of lesional SSc fibroblast, specifically. RELEVANCE TO CLINICAL CARE: This minireview summarizes recent findings pertinent to the origin of myofibroblasts in SSc and how this knowledge might be used to control the fibrosis in this disease. CONCLUSIONS: TGFbeta, ET-1, CCN2 and PDGF are likely to cooperate in driving tissue repair and fibrogenic responses in fibroblasts. TGFbeta, ET-1 and CCN2 appear to contribute to myofibroblast differentiation; PDGF appears to be involved with pericyte recruitment. Thus, different therapeutic strategies may exist for targeting the multisystem fibrotic disorder SSc

Structural Elucidation of Cisoid and Transoid Cyclization Pathways of a Sesquiterpene Synthase Using 2-Fluorofarnesyl Diphosphates

Sesquiterpene skeletal complexity in nature originates from the enzyme-catalyzed ionization of (trans,trans)-farnesyl diphosphate (FPP) (1a) and subsequent cyclization along either 2,3-transoid or 2,3-cisoid farnesyl cation pathways. Tobacco 5-epi-aristolochene synthase (TEAS), a transoid synthase, produces cisoid products as a component of its minor product spectrum. To investigate the cryptic cisoid cyclization pathway in TEAS, we employed (cis,trans)-FPP (1b) as an alternative substrate. Strikingly, TEAS was catalytically robust in the enzymatic conversion of (cis,trans)-FPP (1b) to exclusively (≥99.5%) cisoid products. Further, crystallographic characterization of wild-type TEAS and a catalytically promiscuous mutant (M4 TEAS) with 2-fluoro analogues of both all-trans FPP (1a) and (cis,trans)-FPP (1b) revealed binding modes consistent with preorganization of the farnesyl chain. These results provide a structural glimpse into both cisoid and transoid cyclization pathways efficiently templated by a single enzyme active site, consistent with the recently elucidated stereochemistry of the cisoid products. Further, computational studies using density functional theory calculations reveal concerted, highly asynchronous cyclization pathways leading to the major cisoid cyclization products. The implications of these discoveries for expanded sesquiterpene diversity in nature are discussed

Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular β-glucosidases

The membrane lipid glucosylceramide (GlcCer) is continuously formed and degraded. Cells express two GlcCer-degrading β-glucosidases, GBA and GBA2, located in and outside the lysosome, respectively. Here we demonstrate that through transglucosylation both GBA and GBA2 are able to catalyze in vitro the transfer of glucosyl-moieties from GlcCer to cholesterol, and vice versa. Furthermore, the natural occurrence of 1-O-cholesteryl-β-D-glucopyranoside (GlcChol) in mouse tissues and human plasma is demonstrated using LC-MS/MS and 13C6-labelled GlcChol as internal standard. In cells the inhibition of GBA increases GlcChol, whereas inhibition of GBA2 decreases glucosylated sterol. Similarly, in GBA2-deficient mice GlcChol is reduced. Depletion of GlcCer by inhibition of GlcCer synthase decreases GlcChol in cells and likewise in plasma of inhibitor-treated Gaucher disease patients. In tissues of mice with Niemann-Pick type C, a condition characterized by intralysosomal accumulation of cholesterol, marked elevations in GlcChol occur as well. When lysosomal accumulation of cholesterol is induced in cultured cells, GlcChol is formed via lysosomal GBA. This illustrates that reversible transglucosylation reactions are highly dependent on local availability of suitable acceptors. In conclusion, mammalian tissues contain GlcChol formed by transglucosylation through β-glucosidases using GlcCer as donor. Our findings reveal a novel metabolic function for GlcCer.Bio-organic SynthesisMedical Biochemistr