505 research outputs found

    The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition.

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    Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity

    Magnetic structures and reorientation transitions in noncentrosymmetric uniaxial antiferromagnets

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    A phenomenological theory of magnetic states in noncentrosymmetric tetragonal antiferromagnets is developed, which has to include homogeneous and inhomogeneous terms (Lifshitz-invariants) derived from Dzyaloshinskii-Moriya couplings. Magnetic properties of this class of antiferromagnets with low crystal symmetry are discussed in relation to its first known members, the recently detected compounds Ba2CuGe2O7 and K2V3O8. Crystallographic symmetry and magnetic ordering in these systems allow the simultaneous occurrence of chiral inhomogeneous magnetic structures and weak ferromagnetism. New types of incommensurate magnetic structures are possible, namely, chiral helices with rotation of staggered magnetization and oscillations of the total magnetization. Field-induced reorientation transitions into modulated states have been studied and corresponding phase diagrams are constructed. Structures of magnetic defects (domain-walls and vortices) are discussed. In particular, vortices, i.e. localized non-singular line defects, are stabilized by the inhomogeneous Dzyaloshinskii-Moriya interactions in uniaxial noncentrosymmetric antiferromagnets.Comment: 18 pages RevTeX4, 13 figure

    Systematic first-principles study of impurity hybridization in NiAl

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    We have performed a systematic first-principles computational study of the effects of impurity atoms (boron, carbon, nitrogen, oxygen, silicon, phosporus, and sulfur) on the orbital hybridization and bonding properties in the intermetallic alloy NiAl using a full-potential linear muffin-tin orbital method. The matrix elements in momentum space were used to calculate real-space properties: onsite parameters, partial densities of states, and local charges. In impurity atoms that are empirically known to be embrittler (N and O) we found that the 2s orbital is bound to the impurity and therefore does not participate in the covalent bonding. In contrast, the corresponding 2s orbital is found to be delocalized in the cohesion enhancers (B and C). Each of these impurity atoms is found to acquire a net negative local charge in NiAl irrespective of whether they sit in the Ni or Al site. The embrittler therefore reduces the total number of electrons available for covalent bonding by removing some of the electrons from the neighboring Ni or Al atoms and localizing them at the impurity site. We show that these correlations also hold for silicon, phosporus, and sulfur.Comment: Revtex, 8 pages, 7 eps figures, to appear in Phys. Rev.

    A stage-structured model to predict the effect of temperature and salinity on glass eel Anguilla anguilla pigmentation development

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    The pigmentation development process of glass eels Anguilla anguilla from stage V-B to VIA3 was modelled by gamma cumulative functions. These functions varied with respect to the factors temperature and salinity whose effects were adjusted by beta functions. Temperature was shown to accelerate pigmentation, while salinity acted as a secondary factor slowing down the pigmentation. The model fits the development of 15 samples kept at various temperatures and salinities in the Vilaine River, as well as samples monitored at other dates and places in Europe. It allows the prediction of the duration of estuarine residency for glass eels, in winter and spring, in the Atlantic estuaries

    The initial phase of the 2021 Cumbre Vieja ridge eruption (Canary Islands): Products and dynamics controlling edifice growth and collapse

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    Tajogaite cone in the Cumbre Vieja ridge (La Palma, Canary Islands) erupted between 19 September and 13 December 2021. The tephra and lava sourced from the newly formed fissure rapidly built a pyroclastic cone. During the early days of eruption and after several small-scale landslides, the west flank of the edifice partially collapsed on 25 September, breaching the cone and emplacing a prominent raft-bearing lava flow. Our research combines direct observations, digital elevation models, thermal and visible imaging, and textural and compositional investigation of the explosive products to describe and characterize the edifice growth and collapse. The cone built over a steep slope (26°) and its failure occurred after an intense phase of lava fountaining (up to 30 m3 s−1) that produced rapid pyroclastic accumulation. We suggest that an increased magma supply, to an ascent rate of 0.30 m s−1, led to the rapid growth of the cone (at 2.4 × 106 m3 day−1). Simultaneously, the SW lava flow reactivated and formed a lava ‘seep’ that undercut the flank of the cone, triggering a lateral collapse via rotational rockslide that moved at minimum speeds of 34–70 m h−1. The lateral collapse formed a ~ 200 m wide scar, involving 5.5 × 106 m3 of material, and covered 1.17 km2 with decametric edifice portions and raft-bearing lava. The collapse produced a modest change in the vent geometry, but did not affect eruptive activity long term. A short pause in the eruption after the collapse may have been favored by rapid emptying of the shallower magma system, reducing ascent rates and increasing crystallization times. These results reveal the complex chain of events related to the growth and destruction of newly formed volcanic cones and highlight hazards when situated close to inhabited areas

    Metallothionein (MT) -I and MT-II Expression Are Induced and Cause Zinc Sequestration in the Liver after Brain Injury

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    Experiments with transgenic over-expressing, and null mutant mice have determined that metallothionein-I and -II (MT-I/II) are protective after brain injury. MT-I/II is primarily a zinc-binding protein and it is not known how it provides neuroprotection to the injured brain or where MT-I/II acts to have its effects. MT-I/II is often expressed in the liver under stressful conditions but to date, measurement of MT-I/II expression after brain injury has focused primarily on the injured brain itself. In the present study we measured MT-I/II expression in the liver of mice after cryolesion brain injury by quantitative reverse-transcriptase PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) with the UC1MT antibody. Displacement curves constructed using MT-I/II knockout (MT-I/II−/−) mouse tissues were used to validate the ELISA. Hepatic MT-I and MT-II mRNA levels were significantly increased within 24 hours of brain injury but hepatic MT-I/II protein levels were not significantly increased until 3 days post injury (DPI) and were maximal at the end of the experimental period, 7 DPI. Hepatic zinc content was measured by atomic absorption spectroscopy and was found to decrease at 1 and 3 DPI but returned to normal by 7DPI. Zinc in the livers of MT-I/II−/− mice did not show a return to normal at 7 DPI which suggests that after brain injury, MT-I/II is responsible for sequestering elevated levels of zinc to the liver. Conclusion: MT-I/II is up-regulated in the liver after brain injury and modulates the amount of zinc that is sequestered to the liver

    Challenges for Food Security in Eritrea -- A Descriptive and Qualitative Analysis

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    Food security is about ensuring that all people at all times have both physical and economic access to the basic food they need. In a number of African countries chronic malnutrition and transitory food insecurity are pervasive. Like most African countries, Eritrea is also a victim of the problem of food insecurity. Based on this historical and recurrent food insecurity in Eritrea, an attempt is made in this paper to assess the possible causes of food insecurity in the country. Furthermore, the paper captures the available food security policy proposals of Eritrea and eventually draws conclusions and extends possible recommendations and policy remedies suited to the country
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