Demography and Evolutionary Ecology of the Carnivorous Subshrub Drosophyllum lusitanicum (L.) Link (Drosophyllaceae)

Natural disturbances occur in various ecosystems and have resulted in the evolution of life histories to buffer or even benefit from disturbance regimes. However, human activities increasingly interact with natural disturbances, posing potentially significant threats to the viability of disturbance-adapted species and therefore causing biodiviersity loss. With fires regularly affecting 50 % of the Eath’s surface, such compounded effects of disturbance interactions are particularly prominent in fire-prone ecosystems. Using the rare carnivorous subshrub Drosophyllum lusitanicum (Drosophyllaceae), endemic to Mediterranean heathlands under increasing human pressure in the southwestern Iberian Peninsula and northern Morocco, this doctoral work illustrates how interactions between fire and small-scale human disturbances affect population dynamics and the potential evolutionary trajectory of populations. Greenhouse and in-situ field experiments and stochastic demographic models quantified biological and ecological characteristics of the study species that could be linked to an important, positive role of recurrent fires in population dynamics. At the same time, population censuses across the species range revealed that small-scale human disturbances related to removal of competitively superior shrub neighbors significantly increased the probability of population occurrence and the abundance of several life-cycle stages. Subsequently, stochastic integral projection models confirmed that moderate interactions between human and fire disturbances may significantly improve species viability in the absence of fires. However, a crucial finding of this work was that frequent human disturbances as well as frequent interactions between fires and chronic vegetation removal may be detrimental to population viability because the two fundamentally different disturbance types exert opposing selection pressures on populations. These findings are of potentially great importance for the management of disturbance-adapted species because they highlight the importance of including compounding effects of environmental drivers into demographic models and the need to consider the local disturbance history when designing conservation strategies of species exposed to various disturbance types

Attract them anyway: benefits of large, showy flowers in a highly autogamous, carnivorous plant species

Reproductive biology of carnivorous plants has largely been studied on species that rely on insects as pollinators and prey, creating potential conflicts. Autogamous pollination, although present in some carnivorous species, has received less attention. In angiosperms, autogamous self-fertilization is expected to lead to a reduction in flower size, thereby reducing resource allocation to structures that attract pollinators. A notable exception is the carnivorous pyrophyte Drosophyllum lusitanicum (Drosophyllaceae), which has been described as an autogamous selfing species but produces large, yellow flowers. Using a flower removal and a pollination experiment, we assessed, respectively, whether large flowers in this species may serve as an attracting device to prey insects or whether previously reported high selfing rates for this species in peripheral populations may be lower in more central, less isolated populations. We found no differences between flower-removed plants and intact, flowering plants in numbers of prey insects trapped. We also found no indication of reduced potential for autogamous reproduction, in terms of either seed set or seed size. However, our results showed significant increases in seed set of bagged, hand-pollinated flowers and unbagged flowers exposed to insect visitation compared with bagged, non-manipulated flowers that could only self-pollinate autonomously. Considering that the key life-history strategy of this pyrophytic species is to maintain a viable seed bank, any increase in seed set through insect pollinator activity would increase plant fitness. This in turn would explain the maintenance of large, conspicuous flowers in a highly autogamous, carnivorous plant

Removal of emerging pollutants in conventional and microalgae based biotechnology urban wastewater treatment plants

Wastewater treatment plants (WWTPs) reduce portion of the input of pharmaceuticals in aquatic systems, but there is no data available about the elimination of emerging contaminants with microalgae technology. The aim of this work was to determine the average mass flows and concentrations of pharmaceuticals in influents and effluents from two sewages treatment plants using conventional and microalgae based biotechnologies and to compare the removal of pharmaceuticals using both depuration technologies. Only between 20 to 60% of five pharmaceuticals groups is reduce in both WWTP using conventional technologies consisting of a pretreatment, primary settling and secondary treatment by aerobic biological reactor. Using microalgae based biotechnologies efficiency of removal pharmaceuticals is higher than conventional technologies and it increase by using DAF (Dissolve Air Flotation) technology to separate algae biomass

Pathways to global-change effects on biodiversity: new opportunities for dynamically forecasting demography and species interactions

In structured populations, persistence under environmental change may be particularly threatened when abiotic factors simultaneously negatively affect survival and reproduction of several life cycle stages, as opposed to a single stage. Such effects can then be exacerbated when species interactions generate reciprocal feedbacks between the demographic rates of the different species. Despite the importance of such demographic feedbacks, forecasts that account for them are limited as individual-based data on interacting species are perceived to be essential for such mechanistic forecasting-but are rarely available. Here, we first review the current shortcomings in assessing demographic feedbacks in population and community dynamics. We then present an overview of advances in statistical tools that provide an opportunity to leverage population-level data on abundances of multiple species to infer stage-specific demography. Lastly, we showcase a state-of-the-art Bayesian method to infer and project stage-specific survival and reproduction for several interacting species in a Mediterranean shrub community. This case study shows that climate change threatens populations most strongly by changing the interaction effects of conspecific and heterospecific neighbours on both juvenile and adult survival. Thus, the repurposing of multi-species abundance data for mechanistic forecasting can substantially improve our understanding of emerging threats on biodiversity.12 página

Mediterranean Heathland as a Key Habitat for Fire Adaptations: Evidence from an Experimental Approach

Some fire ecology studies that have focused on garrigue-like vegetation suggest a weak selective pressure of fire in the Mediterranean Basin compared to other Mediterranean-type regions. However, fire-prone Mediterranean heathland from the western end of the Mediterranean Basin has been frequently ignored in the fire ecology literature despite its high proportion of pyrogenic species. Here, we explore the evolutionary ecology of seed traits in the generalist rockrose Cistus salviifolius L. (Cistaceae) aiming to ascertain the role of the Mediterranean heathland for fire adaptations in the Mediterranean Region. We performed a germination experiment to compare the relationship of seed size to (i) heat-stimulated germination, (ii) dormancy strength, and (iii) heat survival in plants from 'high-fire' heathland vs. 'low-fire' coastal shrubland. Germination after heat-shock treatment was higher in large seeds of both 'high-fire' and 'low-fire' habitats. However, dormancy was weaker in small seeds from 'low-fire' habitats. Finally, seed survival to heat shock was positively related to seed size. Our results support that seed size is an adaptive trait to fire in C. salviifolius, since larger seeds had stronger dormancy, higher heat-stimulated germination and were more resistant to heat shock. This seed size-fire relationship was tighter in 'high-fire' Mediterranean heathland than 'low-fire' coastal shrubland, indicating the existence of di fferential fire pressures and evolutionary trends at the landscape scale. These findings highlight the Mediterranean heathland as a relevant habitat for fire-driven evolution, thus contributing to better understand the role of fire in plant evolution within the Mediterranean region

Effects of extreme rainfall events on the distribution of selected emerging contaminants in surface and groundwater: The Guadalete River basin (SW, Spain)

This study is focused on the Guadalete River basin (SW, Spain), where extreme weather conditions have become common, with and alternation between periods of drought and extreme rainfall events. Combined sewer overflows (CSOs) occur when heavy rainfall events exceed the capacity of the wastewater treatment plants (WWTP), as well as pollution episodes in parts of the basin due to uncontrolled sewage spills and the use of reclaimed water and sludge from the local WWTP. The sampling was carried out along two seasons and three campaigns during dry (March 2007) and extreme rainfall (April and December 2010) in the Guadalete River, alluvial aquifer and Jerez de la Frontera aquifer. Results showed minimum concentrations for synthetic surfactants in groundwater (< 37.4 μg·L− 1) during the first campaign (dry weather conditions), whereas groundwater contaminants increased in December 2010 as the heavy rainfall caused the river to overflow. In surface water, surfactant concentrations showed similar trends to groundwater observations. In addition to surfactants, pharmaceuticals and personal care products (PPCPs) were analyzed in the third campaign, 22 of which were detected in surface waters. Two fragrances (OTNE and galaxolide) and one analgesic/anti-inflammatory (ibuprofen) were the most abundant PPCPs (up to 6540, 2748 and 1747 ng·L− 1, respectively). Regarding groundwater, most PPCPs were detected in Jerez de la Frontera aquifer, where a synthetic fragrance (OTNE) was predominant (up to 1285 ng·L− 1)