Concentrating n-3 fatty acids from crude and refined commercial salmon oil

8 páginas, 2 figuras, 2 tablas.-- Mª Elsa Pando ... et al.The urea complexation was used to concentrate n-3 fatty acids (FA) from crude and refined commercial salmon oils. The experimental procedure included salmon oil saponification, free fatty acid (FFA) collection, formation of urea-FFA inclusion complexes, extraction of free n-3 FA and further analysis by gas-liquid chromatography of the corresponding FA methyl esters. Differences between crude and refined salmon oil could be observed. Crude oil provided higher typical odour, viscosity and suspension particle values, whereas crude salmon oil showed higher FFA and impurities content while p -anisidine and iodine values, moisture content and formation of conjugated dienes and trienes did not provide any significant differences between both oils; refined oil showed lower a * and b * scores when compared to its counterpart crude oil. Related to the n-3 PUFA concentration, a decrease in saturated fatty acids C 14:0, C 16:0, and C 18:0 and monounsaturated fatty acids C 18:1 9c, and C 18:1 11c, as well as a high yield of n-3 PUFA, EPA+DHA and total PUFA recovering could be observed starting from both crude and refined oils, which confirmed salmon oil to be a profitable source of such highly valuable constituents. Factors such as reaction temperature and ureaFFA ratio showed to be markedly significant to achieve higher value concentrationSupported by FONDECYT (Chile), Project Nº 1120627Peer reviewe

Optimisation of rancidity stability 6 in long-chain PUFA 7 concentrates obtained from a rainbow trout (Oncorhynchus 8 mykiss) by-product

10 páginas, 5 figuras, 2 tablasThis research was focused on the production of polyunsaturated fatty acid concentrates from a farmed rainbow trout (Oncorhynchus mykiss) by-product (i.e. belly muscle). The effect of different process variables (urea/fatty acids (FA) contents ratio, crystallisation time and temperature and stirring speed of the urea/FA mixture) on the lipid oxidation development during the urea complexation process was investigated. For this purpose, an experimental design (26 runs) following the response-surface methodology was developed. As a result, peroxide value and TOTOX index showed to be dependent on the crystallisation time and temperature and the urea/FA ratio, while no influence of the crystallisation stirring speed was detected on both indices; additionally, polyene index was affected by the urea/FA ratio and its interaction with the crystallisation time. An optimised desirability score near 1.0 was attained provided values of 2.8 °C, 3.05 h and 3.57 were applied for crystallisation temperature, crystallisation time and urea/FA ratio, respectivelyThe work was supported by the FONDECYT program (Government of Chile) throughout the Project N° 1120627Peer reviewe

Acylglycerol synthesis including EPA and DHA from rainbow trout (Oncorhynchus mykiss) belly flap oil and caprylic acid catalyzed by Thermomyces lanuginosus lipase under supercritical carbon dioxide

13 pages, 3 figures, 3 tablesSupercritical carbon dioxide (SCCO2) was studied as a medium for the esterification of eicosapentaenoic acid (n-3 C20:5, EPA) or docosahexaenoic acid (n-3 C22:6, DHA) and caprylic acid (C8:0, CA) in structured triacylglycerols (sTAG) using Thermomyces lanuginosus lipase as biocatalyst. Process variables (n-3 long-chain polyunsaturated fatty acid: CA, n-3 LCPUFA:CA content ratio), glycerol content (wt. %), and supercritical time, temperature and pressure were optimized by the Response Surface Methodology through a central composite design of 25–1 + star. Synthesis of sTAG with EPA, DHA and CA under SCCO2 was significantly affected by the n-3 LCPUFA:CA content ratio and supercritical time. MALDI-TOF mass spectrometry revealed that acylglycerols with the highest levels of EPA or DHA content in the sn-2 position were obtained when the following variables conditions were applied: 50% (n-3 LCPUFA:CA content ratio), 40 °C (supercritical temperature), 20 MPa (supercritical pressure), 4 h (supercritical time) and 20.0 wt. % (glycerol concentration). For such experimental conditions, esterification catalyzed by Thermomyces lanuginosus lipase under supercritical carbon dioxide allowed obtaining sTAG synthesized with 54.95% of CA, 11.64% of EPA and 13.77% of DHAThis work was supported by the Comisión Nacional de Investigación Científica y Tecnológica, FONDECYT program (Government of Chile) (Grant number 1120627) and the Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, ChilePeer reviewe

Differential scanning calorimetry analysis of structured acyglycerols obtained from caprylic acid and omega-3 concentrates of rainbow trout (Oncorhynchus mykiss) belly oil

Poster presented at WEFTA Seafood science for a change demand, held in Bilbao from the 9th to the 11th June 2014The thermal behaviour of structured acylglycerols prepared by lipase-mediated esterification of caprylic acid and omega-3 PUFA concentrates from rainbow trout (Oncorhynchus mykiss) belly oil by differential scanning calorimetry (DSC) was studied. The basic objective of the work was to determine the effect of process variables such as caprylic acid/omega-3 PUFA ratio, reaction temperature, reaction time, pressure during carbon-dioxide supercritical extraction and glycerol content in the structured acylglycerols. For it, changes occurring in parameters such as melting profile, melting range, melting onset, enthalpy and melting peak were analysed. A central composite design 25-1 + star of five factors including 28 runs and based on response surface methodology (RSM) was employed for analysing the esterification mediated by immobilized-1,3-specific lipase (Lipozyme TL IM) obtained from Thermomyces lanuginosus under supercritical carbon dioxide conditions. The melting curve was obtained from the program temperature from -80 to 60°C at 2°C/ min. Belly oil used as control showed a melting peak of -7.30°C with an enthalpy value of 123.2 J/g; meantime, melting point of omega-3-PUFA concentrate was -49.47°C, being its enthalpy score 18.7 J/g. The thermal conditions of structured acylglycerols showed to be significantly affected by all esterification process variables. Structured acylglycerols showing the highest properties were obtained when applying a low temperature and a high pressurization value, which led to a melting peak of -36.67°C and to an enthalpy value of 54.1 J/gN

Interesterificación enzimática de aceite de maqui (Aristotelia chilensis) prensado en frío y aceite de "belly" de Trucha Arcoíris (Oncorhynchus mykiss) mediante CO2 supercrítico

Poster.-- XXI Congreso Chileno de Ingeniería Química, Santiago de Chile del 13 al 16 de noviembre del 2023Proyecto Fodecyt Regular nº1221633N

Extracción de aceite proveniente de maqui liofilizado mediante fluido supercrítico CO2 y caracterización de los compuestos antioxidantes

Poster.-- XXI Congreso Chileno de Ingeniería Química, Santiago de Chile del 13 al 16 de noviembre del 2023Proyecto Fodecyt Regular nº1221633N