Assessment of Pesticide Use in Major Vegetables From Farmers\u27 Perception and Knowledge in Dhading District, Nepal

A field study was carried out to assess the pesticide use status in major vegetable crops from farmers\u27 perception and knowledge in Dhading, Nepal in 2019. Field study was carried with 100 commercial farmer\u27s using semi-structure questionnaire by face to face interview. This study was analyzed by categorization of farmers into small holder (51) and large holder (49) groups on the basis of mean area of vegetable cultivation (6.48 ropani). The highest amount of pesticides is needed in tomato in both large holders and small holders according to the farmer\u27s experience. Among the study farmer\u27s, 41% of them spray the pesticides by making a cocktail or mixed method and 56%  follow the waiting period of 3-5 days in both of the cases. A significant positive correlation was found at 5% level of significance between the knowledge and practice pattern of waiting period of the pesticides and negative correlation was found between the Personal Protective equipment score and health hazard score. Headache was the major health hazards faced by the farmers which was higher in small holders (66.7%) as compared to the large holders (46.9%). Mask was the most used PPE by the farmer\u27s i.e. by 83% in overall. Fourty three percent of the farmer\u27s throw the pesticide containers in secret place after using of it.The use of PPE was seen lower in small holders as compared to the large holders. This study reveals the necessities of suitable program and policies regarding the knowledge, safe handling and use of pesticide among the farmer\u27s level

Effect of different spacing and mulching on growth and yield of Okra (Abelmoschus esculentus L.) in Chitwan, Nepal

Okra (Abelmoschus esculentus L.) is one of the most important vegetable crop of Nepal. Its yield and growth parameters are affected by different cultural practices. This study was conducted at Olericulture Farm of Agriculture and Forestry University, Rampur, Chitwan, Nepal during April 29 to July 9 of 2018. The field experiment was carried out in split plot design using three replications. The treatments consisted of three intra row spacing (30, 45 and 60 cm) and four different mulching materials (Silver plastic, Panicum repens, Lantana camara and bare soil). The objective of this experiment was to assess the effects of various intra-row spacings and mulching materials on growth and yield of okra. The effect of mulching materials on okra yield was found significant. The okra yield was highest (8104 kg/ha) under silver plastic mulch followed by control (5161kg/ha), Panicum repens (3901kg/ha) and Lantana camera (3701kg/ha), respectively. Silver plastic mulch enhanced the growth parameters like canopy length, plant height, leaf number, leaf length, girth and yield of okra. The spacings provided non significant effect on okra yield, however the yield of okra was highest (7295 kg/ha) under 30×30 cm spacing followed by 45×30 cm (4660 kg/ha) and 60 cm × 30 cm spacing (3703 kg/ha), respectively. Combination of silver plastic mulch along with 30 cm × 30 cm spacing provided the highest okra yield. This study suggests that farmers of the Chitwan should grow okra at spacing of 30 cm × 30 cm and under silver plastic mulch to produce higher yield

Efficacy of fungicides in management of Downy mildew disease of Cucumber (Cucumis sativus L.) under open field conditions, in Dhading district of Nepal

Downy mildew (Pseudoperonospora cubensis) is one of the most important disease of cucumber under open field conditions. Hence, the experiment was conducted to study the efficacy of fungicides in management of downy mildew disease of cucumber (Cucumis sativus L.) under open field in Chauradi-7 Dhading during spring season, 2019. Bhaktapur local variety of cucumber was used for the study. The experiment was laid out in single factor randomized complete block design with four replications. Three different bio-fungicides; Trichoderma viride + Trichoderma harzianum, Bacillus subtilis, Verticillium lecanii and one chemical fungicide; Krilaxyl (metalaxyl 8% + mancozeb 64%) were used as treatments. Normal water spray served as control. It was observed that bio-fungicides had insignificant effect in controlling the downy mildew. Pathological observation such as disease incidence percentage, severity percentage and AUDPC in bio-fungicides treated plots were not significantly different from water-sprayed control plots. Similarly, biometric attributes such as number of fruit, length and diameter of fruit, weight of fruit were also non-significant. Krilaxyl resulted significant effect in controlling downy mildew, providing maximum disease reduction (37.48%-50.72%) with lowest disease incidence (1.25-61.25%) and lowest value of AUDPC (138.7). Krilaxyl treated plots had higher number of fruit (6), longer length of fruit (17.15-18.95), higher diameter of fruit (4.16- 4.45 cm) and higher weight of fruit (677 - 759 g). Hence, chemical fungicide Krilaxyl was found better for the management of downy mildew disease in open field cucumber cultivation. Further, researches on bio-fungicides, especially of native strains are required for best result

Assessment of pesticide use in major vegetables from farmers’ perception and knowledge in Dhading district, Nepal

A field study was carried out to assess the pesticide use status in major vegetable crops from farmers’ perception and knowledge in Dhading, Nepal in 2019. Field study was carried with 100 commercial farmer’s using semi-structure questionnaire by face to face interview. This study was analyzed by categorization of farmers into small holder (51) and large holder (49) groups on the basis of mean area of vegetable cultivation (6.48 ropani). The highest amount of pesticides is needed in tomato in both large holders and small holders according to the farmer’s experience. Among the study farmer’s, 41% of them spray the pesticides by making a cocktail or mixed method and 56%  follow the waiting period of 3-5 days in both of the cases. A significant positive correlation was found at 5% level of significance between the knowledge and practice pattern of waiting period of the pesticides and negative correlation was found between the Personal Protective equipment score and health hazard score. Headache was the major health hazards faced by the farmers which was higher in small holders (66.7%) as compared to the large holders (46.9%). Mask was the most used PPE by the farmer’s i.e. by 83% in overall. Fourty three percent of the farmer’s throw the pesticide containers in secret place after using of it.The use of PPE was seen lower in small holders as compared to the large holders. This study reveals the necessities of suitable program and policies regarding the knowledge, safe handling and use of pesticide among the farmer’s level

Effect of Different Spacing and Mulching on Growth and Yield of Okra (Abelmoschus Esculentus L.) in Chitwan, Nepal

Okra (Abelmoschus esculentus L.) is one of the most important vegetable crop of Nepal. Its yield and growth parameters are affected by different cultural practices. This study was conducted at Olericulture Farm of Agriculture and Forestry University, Rampur, Chitwan, Nepal during April 29 to July 9 of 2018. The field experiment was carried out in split plot design using three replications. The treatments consisted of three intra row spacing (30, 45 and 60 cm) and four different mulching materials (Silver plastic, Panicum repens, Lantana camara and bare soil). The objective of this experiment was to assess the effects of various intra-row spacings and mulching materials on growth and yield of okra. The effect of mulching materials on okra yield was found significant. The okra yield was highest (8104 kg/ha) under silver plastic mulch followed by control (5161kg/ha), Panicum repens (3901kg/ha) and Lantana camera (3701kg/ha), respectively. Silver plastic mulch enhanced the growth parameters like canopy length, plant height, leaf number, leaf length, girth and yield of okra. The spacings provided non significant effect on okra yield, however the yield of okra was highest (7295 kg/ha) under 30×30 cm spacing followed by 45×30 cm (4660 kg/ha) and 60 cm × 30 cm spacing (3703 kg/ha), respectively. Combination of silver plastic mulch along with 30 cm × 30 cm spacing provided the highest okra yield. This study suggests that farmers of the Chitwan should grow okra at spacing of 30 cm × 30 cm and under silver plastic mulch to produce higher yield

Случай раннего выявления метастазов в бедренной кости у больного раком полового члена

Background. Bone metastasis is very common in the advanced stage of numerous carcinomas. In penile carcinoma, lymph nodes metastasis is somehow common but it is very rare reported to be secondary from penile cancer. till the date, there are only few cases of penis carcinoma reported bone metastasis in literature worldwide.Case Presentation. Herein, We presented a 51-year-old Nepalese male with squamous cell carcinoma of penis. computed tomography (ct) scan of the patient revealed that there was carcinoma involving glans penis and precure with bilateral external & internal inguinal lymphadenopathies. After then, the patient was under gone for partial penectomy and bilateral inguinal lymphadenectomy and complete 6-cycle chemotherapy. After one year of treatment, patient developed thigh pain and headache and he advised to have magnetic Resonance imaging (mRi) of brain, 99mTc-MDP whole body bone scan and ct scan of pelvis and thigh. The examination report reveals that there was a sclerotic change in vertex of skull bone and moderate 99mTc-MDP uptake in right proximal shaft of femur just below the neck d/d metastasis. The histopathological examination of the true cut biopsy taken from the lesion of the femur showed metastatic keratinizing squamous cell carcinoma which is rare case of femoral shaft bone metastasis secondary from penile carcinoma. Then patient was sent for surgical reconstruction of femur. Based on the case studies review femur shaft bone metastasis from penile cancer is extremely rare.Conclusion. The best of our knowledge; this is the first early detected bone metastases to shaft of the femur in a patient with penile cancer. early diagnosis helps to radical treatment as well as palliative treatment. surgery is the preferred option of the treatments, especially for metastatic foci in the long bones.Аннотация Метастазы в кости часто встречаются на поздних стадиях разных злокачественных новообразований. Для рака полового члена характерно лимфогенное метастазирование, костные метастазы встречаются очень редко. На данный момент в мировой литературе зарегистрировано лишь несколько случаев рака полового члена с метастазами в кости.Описание клинического случая. Мы представляем описание клинического случая плоскоклеточного рака полового члена у 51-летнего мужчины из Непала. Компьютерная томография (КТ) выявила рак полового члена, локализованный на головке и крайней плоти с двусторонней паховой лимфаденопатией. Пациенту выполнена частичная пенэктомия, двусторонняя паховая лимфаденэктомия, проведено 6 курсов адъювантной химиотерапии. Через один год после завершения лечения у пациента появились боли в бедре и головная боль, рекомендована МРТ головного мозга, сканирование костей скелета с 99mTc-MDP и КТ таза и бедра. Обследование показало наличие склеротических изменений в теменной кости черепа и умеренное накопление 99mTc-MDP в проксимальном отделе правом бедренной кости ниже шейки. Гистологическое исследование биоптата, взятого из очага в бедренной кости, выявило метастаз ороговевающего плоскоклеточного рака, что является редким случаем метастазирования рака полового члена в диафиз бедренной кости. Пациент был направлен на хирургическую реконструкцию бедренной кости. Согласно обзору клинических исследований, метастазы в диафизе бедренной кости при раке полового члена встречаются крайне редко.Заключение. Представлен первый случай раннего выявления костных метастазов в диафизе бедренной кости у пациента с раком полового члена. Ранняя диагностика помогает как радикальному, так и паллиативному лечению. Хирургическое вмешательство является предпочтительным вариантом лечения, особенно при метастатических очагах в длинных костях

CSmetaPred: a consensus method for prediction of catalytic residues

Abstract Background Knowledge of catalytic residues can play an essential role in elucidating mechanistic details of an enzyme. However, experimental identification of catalytic residues is a tedious and time-consuming task, which can be expedited by computational predictions. Despite significant development in active-site prediction methods, one of the remaining issues is ranked positions of putative catalytic residues among all ranked residues. In order to improve ranking of catalytic residues and their prediction accuracy, we have developed a meta-approach based method CSmetaPred. In this approach, residues are ranked based on the mean of normalized residue scores derived from four well-known catalytic residue predictors. The mean residue score of CSmetaPred is combined with predicted pocket information to improve prediction performance in meta-predictor, CSmetaPred_poc. Results Both meta-predictors are evaluated on two comprehensive benchmark datasets and three legacy datasets using Receiver Operating Characteristic (ROC) and Precision Recall (PR) curves. The visual and quantitative analysis of ROC and PR curves shows that meta-predictors outperform their constituent methods and CSmetaPred_poc is the best of evaluated methods. For instance, on CSAMAC dataset CSmetaPred_poc (CSmetaPred) achieves highest Mean Average Specificity (MAS), a scalar measure for ROC curve, of 0.97 (0.96). Importantly, median predicted rank of catalytic residues is the lowest (best) for CSmetaPred_poc. Considering residues ranked ≤20 classified as true positive in binary classification, CSmetaPred_poc achieves prediction accuracy of 0.94 on CSAMAC dataset. Moreover, on the same dataset CSmetaPred_poc predicts all catalytic residues within top 20 ranks for ~73% of enzymes. Furthermore, benchmarking of prediction on comparative modelled structures showed that models result in better prediction than only sequence based predictions. These analyses suggest that CSmetaPred_poc is able to rank putative catalytic residues at lower (better) ranked positions, which can facilitate and expedite their experimental characterization. Conclusions The benchmarking studies showed that employing meta-approach in combining residue-level scores derived from well-known catalytic residue predictors can improve prediction accuracy as well as provide improved ranked positions of known catalytic residues. Hence, such predictions can assist experimentalist to prioritize residues for mutational studies in their efforts to characterize catalytic residues. Both meta-predictors are available as webserver at: http://14.139.227.206/csmetapred/

Additional file 1: Table S1. of CSmetaPred: a consensus method for prediction of catalytic residues

Datasets used in present work. List of pdb entries along with known catalytic residues from six datasets. (PDF 576 kb

Current Scenario of Postcholecystectomy Bile Leak and Bile Duct Injury at a Tertiary Care Referral Centre of Nepal

Objective. With the adoption of safe cholecystectomy principles at an academic institute, the risk of major bile duct injury has decreased. This study aims at evaluating the present status of bile duct injury, compared to the study published in 2013 by index centre. Methods. This is a retrospective review of a prospectively maintained database of bile leak and bile duct injury from 2014 to 2019. Patients who completed postcholecystectomy bile leak or bile duct injury treatment and were on regular follow-up were included. Results. Eighteen patients (0.78%) among 2,300 consecutive cholecystectomies presented with bile duct injury, including 8 (0.35%) major bile duct injuries and 10 (0.43%) bile leaks compared to major bile duct injury rate of 0.68% (92/11,345 cholecystectomies) between 2001 and 2010. Injuries were classified as Strasberg’s type A (52.9%), type D (5.9%), and type E (41.1%). Eight patients (47%) of bile leak were managed conservatively with drains, while two required laparotomy and lavage. The mean time for spontaneous closure of bile leak was 11 days. Intraoperative repair was done in three cases: Roux en Y hepaticojejunostomy in 2 and end-to-end repair over T-tube in 1 for sharp transection of the duct. Delayed repair (Roux-en-Y hepaticojejunostomy) was done in five patients. The median postcholecystectomy hospital stay was 8 days, with no mortality. There was no restricture at a median follow-up of 13 months. Conclusion. With the adoption of a safe culture of cholecystectomy, the major bile duct injury rate has decreased currently. Repair of bile duct injury by experienced hepatobiliary surgeon results in excellent outcome