Resolving the Role of Plant Glutamate Dehydrogenase. I. in vivo Real Time Nuclear Magnetic Resonance Spectroscopy Experiments

In higher plants the glutamate dehydrogenase (GDH) enzyme catalyzes the reversible amination of 2-oxoglutarate to form glutamate, using ammonium as a substrate. For a better understanding of the physiological function of GDH either in ammonium assimilation or in the supply of 2-oxoglutarate, we used transgenic tobacco (Nicotiana tabacum L.) plants overexpressing the two genes encoding the enzyme. An in vivo real time 15N-nuclear magnetic resonance (NMR) spectroscopy approach allowed the demonstration that, when the two GDH genes were overexpressed individually or simultaneously, the transgenic plant leaves did not synthesize glutamate in the presence of ammonium when glutamine synthetase (GS) was inhibited. In contrast we confirmed that the primary function of GDH is to deaminate Glu. When the two GDH unlabeled substrates ammonium and Glu were provided simultaneously with either [15N]Glu or 15NH4+ respectively, we found that the ammonium released from the deamination of Glu was reassimilated by the enzyme GS, suggesting the occurrence of a futile cycle recycling both ammonium and Glu. Taken together, these results strongly suggest that the GDH enzyme, in conjunction with NADH-GOGAT, contributes to the control of leaf Glu homeostasis, an amino acid that plays a central signaling and metabolic role at the interface of the carbon and nitrogen assimilatory pathways. Thus, in vivo NMR spectroscopy appears to be an attractive technique to follow the flux of metabolites in both normal and genetically modified plants

Data Management and Processing in Seismology: An Application of Big Data Analysis for the Doublet Earthquake of 2021, 03 March, Elassona, Central Greece

On 3 March 2021 (10:16, UTC), a strong earthquake, Mw 6.3, occurred in Elassona, Central Greece. The epicenter was reported 10 km west of Tyrnavos. Another major earthquake followed this event on the same day at Mw 5.8 (3 March 2021, 11:45, UTC). The next day, 4 March 2021 (18:38, UTC), there was a second event with a similar magnitude as the first, Mw 6.2. Both events were 8.5 km apart. The following analysis shows that the previous events and the most significant aftershocks were superficial. However, historical and modern seismicity has been sparse in this area. Spatially, the region represents a transitional zone between different tectonic domains; the right-lateral slip along the western end of the North Anatolian Fault Zone (NAFZ) in the north Aegean Sea plate-boundary structure ends, and crustal extension prevails in mainland Greece. These earthquakes were followed by rich seismic activity recorded by peripheral seismographs and accelerometers. The installation of a dense, portable network from the Aristotle University of Thessaloniki team also helped this effort, installed three days after the seismic excitation, as seismological stations did not azimuthally enclose the area. In the present work, a detailed analysis was performed using seismological data. A seismological catalogue of 3.787 events was used, which was processed with modern methods to calculate 34 focal mechanisms (Mw > 4.0) and to recalculate the parameters of the largest earthquakes that occurred in the first two days

Data Management and Processing in Seismology: An Application of Big Data Analysis for the Doublet Earthquake of 2021, 03 March, Elassona, Central Greece

On 3 March 2021 (10:16, UTC), a strong earthquake, Mw 6.3, occurred in Elassona, Central Greece. The epicenter was reported 10 km west of Tyrnavos. Another major earthquake followed this event on the same day at Mw 5.8 (3 March 2021, 11:45, UTC). The next day, 4 March 2021 (18:38, UTC), there was a second event with a similar magnitude as the first, Mw 6.2. Both events were 8.5 km apart. The following analysis shows that the previous events and the most significant aftershocks were superficial. However, historical and modern seismicity has been sparse in this area. Spatially, the region represents a transitional zone between different tectonic domains; the right-lateral slip along the western end of the North Anatolian Fault Zone (NAFZ) in the north Aegean Sea plate-boundary structure ends, and crustal extension prevails in mainland Greece. These earthquakes were followed by rich seismic activity recorded by peripheral seismographs and accelerometers. The installation of a dense, portable network from the Aristotle University of Thessaloniki team also helped this effort, installed three days after the seismic excitation, as seismological stations did not azimuthally enclose the area. In the present work, a detailed analysis was performed using seismological data. A seismological catalogue of 3.787 events was used, which was processed with modern methods to calculate 34 focal mechanisms (Mw > 4.0) and to recalculate the parameters of the largest earthquakes that occurred in the first two days