Introduction

This article serves as an introduction for this special issue of the Victoria University of Wellington Law Review including the papers presented at the inaugural New Zealand Private Law Scholars' Roundtable in February 2016.&nbsp

Functional analysis of a human A1 adenosine receptor/green fluorescent protein/Gi1α fusion protein following stable expression in CHO cells

AbstractFusion proteins between the human A1 adenosine receptor and the pertussis toxin resistant (Cys351Gly) mutant of the G-protein α subunit Gi1α (A1/Gi), and between the human A1 adenosine receptor, the Aequorea victoria green fluorescent protein (GFP) and Cys351Gly Gi1α (A1/GFP/Gi), were expressed in CHO cells. The agonist NECA caused a stimulation of [35S]GTPγS binding at both fusion proteins with similar concentration dependence as at the native receptor. However in the presence of pertussis toxin NECA stimulation of [35S]GTPγS binding was only seen at the A1/GFP/Gi fusion protein. The regulation of the adenylyl cyclase and MAP kinase effector systems by both fusion proteins was attenuated following pertussis toxin treatment. These studies demonstrate for the first time the characterisation of a fusion protein between a G-protein coupled receptor, GFP and a G-protein α subunit

Global Scale Variation in the Salinity Sensitivity of Riverine Macroinvertebrates: Eastern Australia, France, Israel and South Africa

Salinity is a key abiotic property of inland waters; it has a major influence on biotic communities and is affected by many natural and anthropogenic processes. Salinity of inland waters tends to increase with aridity, and biota of inland waters may have evolved greater salt tolerance in more arid regions. Here we compare the sensitivity of stream macroinvertebrate species to salinity from a relatively wet region in France (Lorraine and Brittany) to that in three relatively arid regions eastern Australia (Victoria, Queensland and Tasmania), South Africa (south-east of the Eastern Cape Province) and Israel using the identical experimental method in all locations. The species whose salinity tolerance was tested, were somewhat more salt tolerant in eastern Australia and South Africa than France, with those in Israel being intermediate. However, by far the greatest source of variation in species sensitivity was between taxonomic groups (Order and Class) and not between the regions. We used a bayesian statistical model to estimate the species sensitivity distributions (SSDs) for salinity in eastern Australia and France adjusting for the assemblages of species in these regions. The assemblage in France was slightly more salinity sensitive than that in eastern Australia. We therefore suggest that regional salinity sensitivity is therefore likely to depend most on the taxonomic composition of respective macroinvertebrate assemblages. On this basis it would be possible to screen rivers globally for risk from salinisation

Soil property differences and irrigated-cotton lint yield— Cause and effect? An on-farm case study across three cotton-growing regions in Australia

The average lint yield of irrigated cotton in Australia ranges from 2270 to 3700kg/ ha, but yields vary substantially between farms and also between fields on the same farm. Differences in soil properties may cause these yield variations. Identifying which factors are causal and what management can be implemented to mitigate the impacts should help optimize inputs and improve profits. During the 2018–2019 summer cotton-growing season, a paired-field comparison approach was used to investigate and improve the understanding of soil property induced irrigated cotton yield differences within five farms across three regions of NSW, Australia. The paired fields at each farm recorded an average lint yield difference of >284kg/ha (measured in 2018–2019 or 5-year average lint yield). Several soil properties differed between the paired fields at each farm comparison. The soil organic carbon stocks were higher in the higher-yielding fields at all the farm comparisons and the normalized lint yield percentage was positively correlated with soil organic carbon stocks. Soil sodicity was higher in the lower yielding fields at 3 of the 5 comparisons. Results for most soil nutrient tests were above the recommended critical concentrations for Australian cotton production. A stepwise linear regression excluding soil nutrients that were above soil test critical values for crop response and below crop toxicity levels indicated the lint yield was positively correlated with SOC stocks and negatively correlated with sodicity and bulk density. No earthworms were detected during visual soil assessment or soil sampling across all the sites. Visual soil assessment was not a sensitive predictor of cotton crop performance. Comparing soil properties using a paired field approach may assist cotton growers in understanding the factors behind yield differences. A similar strip comparison approach could be adopted for within-field variability by dividing the fields into discrete performance zones and assessing the soil properties of each zone separately.284kg/ha (measured in 2018–2019 or 5-year average lint yield). Several soil properties differed between the paired fields at each farm comparison. The soil organic carbon stocks were higher in the higher-yielding fields at all the farm comparisons and the normalized lint yield percentage was positively correlated with soil organic carbon stocks. Soil sodicity was higher in the lower yielding fields at 3 of the 5 comparisons. Results for most soil nutrient tests were above the recommended critical concentrations for Australian cotton production. A stepwise linear regression excluding soil nutrients that were above soil test critical values for crop response and below crop toxicity levels indicated the lint yield was positively correlated with SOC stocks and negatively correlated with sodicity and bulk density. No earthworms were detected during visual soil assessment or soil sampling across all the sites. Visual soil assessment was not a sensitive predictor of cotton crop performance. Comparing soil properties using a paired field approach may assist cotton growers in understanding the factors behind yield differences. A similar strip comparison approach could be adopted for within-field variability by dividing the fields into discrete performance zones and assessing the soil properties of each zone separately

Beyond the 'Field of Dreams' model in smallholder forestry: building viable timber value chains for smallholder tree growers in developing countries

Many smallholder tree growers in developing countries and those advising them, hold a view that if they plant trees a market will materialize when the time is right. However, despite strong international demand for timber and potential for smallholders to supply this demand, this ‘Field of Dreams’ approach, i.e. if you grow it, buyers will come, is not generally a sound strategy. In this study, we aimed to identify the conditions that enable the development of viable timber value chains around smallholder tree growers in developing countries. We reviewed literature on the integration of small-scale producers into value chains, smallholder tree growing, and smallholder commercial forestry to identify conditions, and used four case studies in the Asia-Pacific Region to understand how these influence outcomes for smallholder tree growers in different settings. This analysis provided a basis for recommendations for policymakers and advising agencies on how to support timber value chains for smallholder tree growers. These included deeper understanding of biophysical suitability of locations for tree growing, smallholder capabilities and interests, and provision of clear land tenure, infrastructure, and streamlined regulations sympathetic to smallscale timber operations. Tree growing can generate financial value for smallholders in regions with high human population density, quality road networks and proximity to processing markets and ports. Careful policy design is required to make it ‘fit for purpose’ at local levels, as conditions vary widely even within a single country. This can identify catalytic interventions and work with existing or near-term market drivers and simplified regulations in the value chain to generate benefits for smallholders

Application of pharmacogenomics and bioinformatics to exemplify the utility of human <i>ex vivo</i> organoculture models in the field of precision medicine

Here we describe a collaboration between industry, the National Health Service (NHS) and academia that sought to demonstrate how early understanding of both pharmacology and genomics can improve strategies for the development of precision medicines. Diseased tissue ethically acquired from patients suffering from chronic obstructive pulmonary disease (COPD), was used to investigate inter-patient variability in drug efficacy using ex vivo organocultures of fresh lung tissue as the test system. The reduction in inflammatory cytokines in the presence of various test drugs was used as the measure of drug efficacy and the individual patient responses were then matched against genotype and microRNA profiles in an attempt to identify unique predictors of drug responsiveness. Our findings suggest that genetic variation in CYP2E1 and SMAD3 genes may partly explain the observed variation in drug response

Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting

Aims: To compare the constitutive and agonist-dependent endosomal trafficking of α1A- and α1B-adrenoceptors (ARs) and to establish if the internalization pattern determines the signaling pathways of each subtype. Methods: Using CypHer5 technology and VSV-G epitope tagged α1A- and α1B-ARs stably and transiently expressed in HEK 293 cells, we analyzed by confocal microscopy the constitutive and agonist-induced internalization of each subtype, and the temporal relationship between agonist induced internalization and the increase in intracellular calcium (determined by FLUO-3 flouorescence), or the phosphorylation of ERK1/2 and p38 MAP kinases (determined by Western blot). Results and Conclusions: Constitutive as well as agonist-induced trafficking of α1A and α1B ARs maintain two different endosomal pools of receptors: one located close to the plasma membrane and the other deeper into the cytosol. Each subtype exhibited specific characteristics of internalization and distribution between these pools that determines their signaling pathways: α1A-ARs, when located in the plasma membrane, signal through calcium and ERK1/2 pathways but, when translocated to deeper endosomes, through a mechanism sensitive to β-arrestin and concanavalin A, continue signaling through ERK1/2 and also activate the p38 pathway. α1B-ARs signal through calcium and ERK1/2 only when located in the membrane and the signals disappear after endocytosis and by disruption of the membrane lipid rafts by methyl-β-cyclodextrin

Assessing drivers of plantation forest productivity on eroded and non-eroded soils in hilly land, eastern North Island, New Zealand

Methods: The impact of soil erosion by mass movement on forest productivity was investigated in a paired plot trial in a planted forest in a mainly hilly to steepland catchment (Pakuratahi) near Napier, eastern North Island, New Zealand. Tree growth and form were measured and soil properties analysed to compare productivity and productivity drivers in adjacent non-eroded and eroded plots. Background: The effect of soil erosion on New Zealand production forestry is not well known and there has been no research prior to our study into the relationship between soil nutrient status and planted forests growing in eroded soils in steeplands. Results: Regression analysis showed that the decreased soil total nitrogen, total carbon, total phosphorus, and soil organic matter content in eroded plots had a negative impact on tree volume, resulting in a 10% decrease in measured tree volume. Based on an assessment of log quality, trees in the eroded plots were forecast to produce 16% less volume from high-quality pruned logs (with associated reduction in revenue of around $4000 per hectare), than trees in non-eroded plots. The total recoverable volume (TRV), estimated (for a 25-year rotation) from the measured Pinus radiata D. Don trees growing on the eroded sites, was valued at$68,500, about 9% less than the estimated TRV from trees measured on non-eroded plots ($76,000). Tree form and mean tree height in eroded and non-eroded plots were not significantly different. Conclusions: Soil erosion impacts production in planted forests. Afforestation of erodible land provides a valuable ecosystem service through land and soil stabilisation but this service is currently not reflected in the market prices for timber in New Zealand. Maintaining the productive capacity of erodible soils through practices such as fertilisation or continuous-cover forestry can add further costs to production forestry. To ensure that sustainable forest practices are carried out to protect the productivity of soils, financial incentives may be justified