201 research outputs found
Platinum Integrated Graphene for Methanol Fuel Cells
Uniform and porous graphene nanoflake films (GNFs) have been investigated as a support for catalytic Pt nanoclusters in direct methanol electro-oxidation. Pt nanoclusters of varying thickness are deposited on GNFs using magnetron sputtering, and their effects on the electrocatalytic activity for oxidizing methanol are systemically studied. GNF supported Pt nanoclusters with ultralow catalyst loading exhibit high performance for methanol electrocatalytic oxidation with a large mass-specific peak current density and a ratio of forward to backward peak currents up to 1.4. These characteristics compare favorably to the majority of PtâC based electrodes, except for those of carbon nanotubes with Pt decoration on both the inner and the outer wall surfaces. The results obtained are ascribed to a highly coupled network made of high-density 2â4 nm Pt monolayer nanoclusters on both the basal and edge planes of each nanoflakes of graphene. GNFs are a promising support material for developing next-generation advanced Pt based fuel cells and their relevant electrodes in the field of energy
Genetic Contributions to the Association between Adult Height and Head and Neck Cancer : A Mendelian Randomization Analysis
Acknowledgements: The authors thank the Italian Association for Research on Cancer (AIRC, IG 2013 n. 14211) and the PRECeDI project (Marie SkĆodowska-Curie Research and Innovation Staff Exchange - RISE N°645740) for supporting the work of Roberta Pastorino and Anna Puggina.Peer reviewedPublisher PD
Probing the Thermal Deoxygenation of Graphene Oxide using High Resolution In Situ X-Ray based Spectroscopies
Despite the recent developments in Graphene Oxide due to its importance as a
host precursor of Graphene, the detailed electronic structure and its evolution
during the thermal reduction remain largely unknown, hindering its potential
applications. We show that a combination of high resolution in situ X-ray
photoemission and X-ray absorption spectroscopies offer a powerful approach to
monitor the deoxygenation process and comprehensively evaluate the electronic
structure of Graphene Oxide thin films at different stages of the thermal
reduction process. It is established that the edge plane carboxyl groups are
highly unstable, whereas carbonyl groups are more difficult to remove. The
results consistently support the formation of phenol groups through reaction of
basal plane epoxide groups with adjacent hydroxyl groups at moderate degrees of
thermal activation (~400 {\deg}C). The phenol groups are predominant over
carbonyl groups and survive even at a temperature of 1000 {\deg}C. For the
first time a drastic increase in the density of states (DOS) near the Fermi
level at 600 {\deg}C is observed, suggesting a progressive restoration of
aromatic structure in the thermally reduced graphene oxideComment: Pagona Papakonstantinou as Corresponding author, E-mail:
[email protected]
Central Role of SREBP-2 in the Pathogenesis of Osteoarthritis
Background: Recent studies have implied that osteoarthritis (OA) is a metabolic disease linked to deregulation of genes involved in lipid metabolism and cholesterol efflux. Sterol Regulatory Element Binding Proteins (SREBPs) are transcription factors regulating lipid metabolism with so far no association with OA. Our aim was to test the hypothesis that SREBP-2, a gene that plays a key role in cholesterol homeostasis, is crucially involved in OA pathogenesis and to identify possible mechanisms of action. Methodology/Principal Findings: We performed a genetic association analysis using a cohort of 1,410 Greek OA patients and healthy controls and found significant association between single nucleotide polymorphism (SNP) 1784G>C in SREBP-2 gene and OA development. Moreover, the above SNP was functionally active, as normal chondrocytesâ transfection with SREBP-2-G/C plasmid resulted in interleukin-1ÎČ and metalloproteinase-13 (MMP-13) upregulation. We also evaluated SREBP-2, its target gene 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGCR), phospho-phosphoinositide3-kinase (PI3K), phospho-Akt, integrin-alphaV (ITGAV) and transforming growth factor- (TGF-) mRNA and protein expression levels in osteoarthritic and normal chondrocytes and found that they were all significantly elevated in OA chondrocytes. To test whether TGF- alone can induce SREBP-2, we treated normal chondrocytes with TGF- and found significant upregulation of SREBP-2, HMGCR, phospho-PI3K and MMP-13. We also showed that TGF- activated aggrecan (ACAN) in chondrocytes only through Smad3, which interacts with SREBP-2. Finally, we examined the effect of an integrin inhibitor, cyclo-RGDFV peptide, on osteoarthritic chondrocytes, and found that it resulted in significant upregulation of ACAN and downregulation of SREBP-2, HMGCR, phospho-PI3K and MMP-13 expression levels. Conclusions/Significance: We demonstrated, for the first time, the association of SREBP-2 with OA pathogenesis and provided evidence on the molecular mechanism involved. We suggest that TGF- induces SREBP-2 pathway activation through ITGAV and PI3K playing a key role in OA and that integrin blockage may be a potential molecular target for OA treatment
Integrative MicroRNA and Proteomic Approaches Identify Novel Osteoarthritis Genes and Their Collaborative Metabolic and Inflammatory Networks
BACKGROUND: Osteoarthritis is a multifactorial disease characterized by destruction of the articular cartilage due to genetic, mechanical and environmental components affecting more than 100 million individuals all over the world. Despite the high prevalence of the disease, the absence of large-scale molecular studies limits our ability to understand the molecular pathobiology of osteoathritis and identify targets for drug development. METHODOLOGY/PRINCIPAL FINDINGS: In this study we integrated genetic, bioinformatic and proteomic approaches in order to identify new genes and their collaborative networks involved in osteoarthritis pathogenesis. MicroRNA profiling of patient-derived osteoarthritic cartilage in comparison to normal cartilage, revealed a 16 microRNA osteoarthritis gene signature. Using reverse-phase protein arrays in the same tissues we detected 76 differentially expressed proteins between osteoarthritic and normal chondrocytes. Proteins such as SOX11, FGF23, KLF6, WWOX and GDF15 not implicated previously in the genesis of osteoarthritis were identified. Integration of microRNA and proteomic data with microRNA gene-target prediction algorithms, generated a potential "interactome" network consisting of 11 microRNAs and 58 proteins linked by 414 potential functional associations. Comparison of the molecular and clinical data, revealed specific microRNAs (miR-22, miR-103) and proteins (PPARA, BMP7, IL1B) to be highly correlated with Body Mass Index (BMI). Experimental validation revealed that miR-22 regulated PPARA and BMP7 expression and its inhibition blocked inflammatory and catabolic changes in osteoarthritic chondrocytes. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that obesity and inflammation are related to osteoarthritis, a metabolic disease affected by microRNA deregulation. Gene network approaches provide new insights for elucidating the complexity of diseases such as osteoarthritis. The integration of microRNA, proteomic and clinical data provides a detailed picture of how a network state is correlated with disease and furthermore leads to the development of new treatments. This strategy will help to improve the understanding of the pathogenesis of multifactorial diseases such as osteoarthritis and provide possible novel therapeutic targets
Plasma antibodies to oral bacteria and risk of pancreatic cancer in a large European prospective cohort study
Objective: Examine the relationship between antibodies to 25 oral bacteria and pancreatic cancer risk in a prospective cohort study. Design: We measured antibodies to oral bacteria in prediagnosis blood samples from 405 pancreatic cancer cases and 416 matched controls, nested within the European Prospective Investigation into Cancer and Nutrition study. Analyses were conducted using conditional logistic regression and additionally adjusted for smoking status and body mass index. Results: Individuals with high levels of antibodies against Porphyromonas gingivalis ATTC 53978, a pathogenic periodontal bacteria, had a twofold higher risk of pancreatic cancer than individuals with lower levels of these antibodies (OR 2.14; 95% CI 1.05 to 4.36; >200ng/ml vs 200ng/ml). To explore the association with commensal (non-pathogenic) oral bacteria, we performed a cluster analysis and identified two groups of individuals, based on their antibody profiles. A cluster with overall higher levels of antibodies had a 45% lower risk of pancreatic cancer than a cluster with overall lower levels of antibodies (OR 0.55; 95% CI 0.36 to 0.83). Conclusion: Periodontal disease might increase the risk for pancreatic cancer. Moreover, increased levels of antibodies against specific commensal oral bacteria, which can inhibit growth of pathogenic bacteria, might reduce the risk of pancreatic cancer. Studies are needed to determine whether oral bacteria have direct effects on pancreatic cancer pathogenesis or serve as markers of the immune response
Meat and haem iron intake in relation to glioma in the European Prospective Investigation into Cancer and Nutrition study
Diets high in red or processed meat have been associated positively with some cancers, and several possible underlying mechanisms have been proposed, including iron-related pathways. However, the role of meat intake in adult glioma risk has yielded conflicting findings because of small sample sizes and heterogeneous tumour classifications. The aim of this study was to examine red meat, processed meat and iron intake in relation to glioma risk in the European Prospective Investigation into Cancer and Nutrition study. In this prospective cohort study, 408751 individuals from nine European countries completed demographic and dietary questionnaires at recruitment. Multivariable Cox proportional hazards models were used to examine intake of red meat, processed meat, total dietary iron and haem iron in relation to incident glioma. During an average follow-up of 14.1 years, 688 incident glioma cases were diagnosed. There was no evidence that any of the meat variables (red, processed meat or subtypes of meat) or iron (total or haem) were associated with glioma; results were unchanged when the first 2 years of follow-up were excluded. This study suggests that there is no association between meat or iron intake and adult glioma. This is the largest prospective analysis of meat and iron in relation to glioma and as such provides a substantial contribution to a limited and inconsistent literature
Tuning the Catalytic Activity of Graphene Nanosheets for Oxygen Reduction Reaction via Size and Thickness Reduction
Currently, the fundamental factors that control the oxygen reduction reaction
(ORR) activity of graphene itself, in particular the dependence of the ORR
activity on the number of exposed edge sites remain elusive, mainly due to
limited synthesis routes of achieving small size graphene. In this work, the
synthesis of low oxygen content (< 2.5 +/-0.2 at %), few layer graphene
nanosheets with lateral dimensions smaller than a few hundred nm was achieved
using a combination of ionic liquid assisted grinding of high purity graphite
coupled with sequential centrifugation. We show for the first time, that the
graphene nanosheets possessing a plethora of edges exhibited considerably
higher electron transfer numbers compared to the thicker graphene
nanoplatelets. This enhanced ORR activity was accomplished by successfully
exploiting the plethora of edges of the nanosized graphene as well as the
efficient electron communication between the active edge sites and the
electrode substrate. The graphene nanosheets were characterized by an onset
potential of -0.13 V vs. Ag/AgCl and a current density of -3.85 mA/cm2 at -1 V,
which represent the best ORR performance ever achieved from an undoped carbon
based catalyst. This work demonstrates how low oxygen content nanosized
graphene synthesized by a simple route can considerably impact the ORR
catalytic activity and hence it is of significance in designing and optimizing
advanced metal-free ORR electrocatalysts.Comment: corresponding author: [email protected], ACS Applied
Materials and Interfaces 201
Coffee consumption and gastric cancer: a pooled analysis from the Stomach cancer Pooling Project consortium
Objective: This study aimed to evaluate and quantify the relationship between coffee and gastric cancer using a uniquely large dataset from an international consortium of observational studies on gastric cancer, including data from 18 studies, for a total of 8198 cases and 21 419 controls.
Methods: A two-stage approach was used to obtain the pooled odds ratios (ORs) and the corresponding 95% confidence intervals (CIs) for coffee drinkers versus never or rare drinkers. A one-stage logistic mixed-effects model with a random intercept for each study was used to estimate the dose-response relationship. Estimates were adjusted for sex, age and the main recognized risk factors for gastric cancer.
Results: Compared to never or rare coffee drinkers, the estimated pooled OR for coffee drinkers was 1.03 (95% CI, 0.94-1.13). When the amount of coffee intake was considered, the pooled ORs were 0.91 (95% CI, 0.81-1.03) for drinkers of 1-2 cups per day, 0.95 (95% CI, 0.82-1.10) for 3-4 cups, and 0.95 (95% CI, 0.79-1.15) for five or more cups. An OR of 1.20 (95% CI, 0.91-1.58) was found for heavy coffee drinkers (seven or more cups of caffeinated coffee per day). A positive association emerged for high coffee intake (five or more cups per day) for gastric cardia cancer only.
Conclusions: These findings better quantify the previously available evidence of the absence of a relevant association between coffee consumption and gastric cancer
Zinc PhthalocyanineâGraphene Hybrid Material for Energy Conversion: Synthesis, Characterization, Photophysics and Photoelectrochemical Cell Preparation
Graphene exfoliation upon tip sonication in o-ÂâDCB was accomplished. Then, covalent grafting of (2-Ââ aminoethoxy)(tri-Ââtert-Ââbutyl) zinc phthalocyanine (ZnPc), to exfoliated graphene sheets was achieved. The newly formed ZnPc-Ââgraphene hybrid material was found soluble in common organic solvents without any precipitation for several weeks. Application of diverse spectroscopic techniques verified the successful formation of ZnPc-Ââgraphene hybrid materi-Ââ al, while thermogravimetric analysis revealed the amount of ZnPc loading onto graphene. Microscopy analysis based on AFM and TEM was applied to probe the morphological characteristics and to investigate the exfoliation of graphene sheets. Efficient fluorescence quenching of ZnPc in the ZnPc-Ââgraphene hybrid material suggested that photoinduced events occur from the photoexcited ZnPc to exfoliated graphene. The dynamics of the photoinduced electron transfer was
evaluated by femtosecond transient absorption spectroscopy, thus, revealing the formation of transient species such as ZnPc+ yielding the charge-Ââseparated state ZnPcâą+âgrapheneâąâ. Finally, the ZnPc-Ââgraphene hybrid material was integrated into a photoactive electrode of an optical transparent electrode (OTE) cast with nanostructured SnO2 films (OTE/SnO2), which exhibited sta le and reproducible photocurrent responses and the incident photon-Ââto-Ââcurrent conversion efficien-Ââ cy was determine
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