Genetic diversity studies in common bean (Phaseolus vulgaris L.) using molecular markers

Molecular characterization of thirteen common bean genotypes was done with random amplified polymorphic DNA (RAPD) markers. Initially, 15 primers were screened out of which only seven were selected which generated a total of 65 amplification products out of which 63 bands (96.62%) were polymorphic indicating fair amount of polymorphism. The genotypes shared 43% genetic similarity among themselves. Cluster analysis delineated the genotypes into three groups with seven, five and one genotype in cluster-I, II and III, respectively. The maximum similarity index (82.35) based dice similarity coefficient was obtained between SKUA-R-21 and SKUA-R-19, while it was minimum (27.72) between genotypes PBG-29 and SKUA-R-01.Key words: Genetic divergence, common bean, random amplified polymorphic DNA (RAPD)

<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Molecular characterization of wheat germplasm for stripe rust resistance genes (<i style="mso-bidi-font-style:normal">Yr5</i>, <i style="mso-bidi-font-style: normal">Yr10</i>, <i style="mso-bidi-font-style:normal">Yr15</i> & <i style="mso-bidi-font-style:normal">Yr18</i>) and identification of candidate lines for stripe rust breeding in Kashmir</span>

241-248<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important disease of wheat in Jammu and Kashmir state of India. The leading cultivars and breeding lines of wheat in the state were evaluated for stripe rust resistance at the experimental fields of the Faculty of Agriculture, Wadura. The study on molecular characterization and identification of candidate lines for stripe rust resistance genes <i style="mso-bidi-font-style: normal">Yr5, Yr10, Yr15 and <i style="mso-bidi-font-style: normal">Yr18 depicted that gene Yr5 was found in 14 entries, Yr10 in 29 entries, Yr15 in 25 entries and Yr18 in 9 entries including reference lines, while the susceptible control did not amplify any of the resistance genes used in the study. Single gene based resistance was detected in 9 entries, two gene based resistance was detected in 20 cultivars, three gene based resistance was detected in 8 cultivars and all four gene based resistance was detected in 1 cultivar (HPW-42). Such validation information is valuable and can efficiently be used in devising future breeding strategies in building a long lasting defense against stripe rust fungus.</span