The role of functional prototyping within the KADS methodology : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Computer Science at Massey University

Knowledge-based systems have until recent times lacked a clear and complete methodology for their construction. KADS was the result of the early 1980's project (ESPRIT-I P1098) which had the aim of developing a comprehensive, commercially viable methodology for knowledge-based system construction. KADS has subsequently proved to be one of the more popular approaches, focusing on the modelling approach to knowledge based system development. One area of the KADS methodology that has not been examined to any great depth is that of model validation. Model validation is the process of ensuring that a derived model is an accurate representation of the domain from which it has been derived from. The two approaches which have been suggested for this purpose within the KADS framework are that of protocol analysis and functional prototyping. This project seeks to apply the second of these choices, that of functional prototyping, to the model of expertise created by da Silva (1994) for model validation purposes. The problem domain is that of farm management, under an joint program of research between the Computer Science, Information Systems and Agricultural Management departments of Massey University. The project took the model of expertise and created a knowledge representation model in compliance with the selected object-oriented paradigm. After this the creation of a functional prototype in a Microsoft Windows based PC environment took place, using Kappa-PC as the application development tool. The validation took place through a demonstration session to a number of domain experts. Conclusions drawn from the experience gained through the creation and use of the prototype are presented, outlining the reasons why functional prototyping was deemed to be an appropriate method for model validation

Lived Experiences of College Football Fans in the Southeast United States: An Interpretative Phenomenological Analysis

While a number of sport-related studies have explored sport fandom and its effects quantitatively, there is a lack of research that exists surrounding the lived experiences of fans, and thus any potential health-related benefits derived through fandom. The present study qualitatively explored the lived experiences of college football fans in the Southeast United States with aims to identify any potential health related benefits derived through fandom experiences. Eight participants were interviewed utilizing semi-structured interviews. Interviews were analyzed via interpretative phenomenological analysis. Results are discussed within the context of positive psychology and identity theory and suggest that participants experienced phycological and social well-being benefits as a result of their football fandom experiences and rituals. Furthermore, with respect to identity theory, results suggest that being a fan of the team provided a sense of self-purpose or meaning to participants that goes beyond just enjoyment for the game

CXCL12‐induced macropinocytosis modulates two distinct pathways to activate mTORC1 in macrophages

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141686/1/jlb0683.pd

Porencephaly in an Italian neonate with foetal alcohol spectrum disorder: A case report

INTRODUCTION: Foetal alcohol spectrum disorder (FASD) is a complex malformative disease caused by the teratogenic effect of alcohol consumed during pregnancy. Mothers are frequently reluctant to admit alcohol consumption during pregnancy. During infancy and particularly during neonatal period, differential diagnosis is difficult. PATIENT CONCERNS: This case is represented by an Italian neonate boy small for gestational age, born by caesarean section at a gestational age of 37 weeks + 6 days by neglect and single-parent pregnancy. On physical examination, he presented particular facial features: microcephaly, epicanthal folds, flat midface, low nasal bridge, indistinct philtrum, and thin upper lip; moreover, examination revealed a macro-penis and recurvation without evidence of glans. DIAGNOSIS: Echocardiogram showed an inter-ventricular defect of medium-muscular type and brain magnetic resonance imaging showed asymmetry of the cerebral hemispheres with hypoplasia of the left cerebral hemisphere, dilatation of the left ventricle, cerebrospinal fluid cavity, and porencephaly. INTERVENTIONS: We investigated the ethylglucuronide (EtG) concentration in the neonate's hair by liquid chromatography-tandem mass spectrometry and we detected EtG in the infant's hair (normal value, 30 pg/mg), demonstrating prenatal alcohol exposure. OUTCOMES: In this neonate, EtG measure in hairs permitted the diagnosis of FASD, so allowing to exclude genetic diseases associated with similar clinical findings. After this result the mother admitted that she drunk alcohol during pregnancy (she declared 3 glasses of wine every day). At the age of 6 months, the child showed a moderate neurodevelopmental delay. CONCLUSION: This case shows that FAD should be considered in neonates with rare neurological diseases as porencephaly. In neonates and infants born to a mother who did not report alcohol use, EtG measure in hairs can significantly improve diagnosis of FASD, so allowing to exclude genetic diseases associated with similar clinical findings

Thermal characterization of recycled materials for building insulation

The building sector is known to have a significant environmental impact, considering that it is the largest contributor to global greenhouse gas emissions of around 36% and is also responsible for about 40% of global energy consumption. Of this, about 50% takes place during the building operational phase, while around 10–20% is consumed in materials manufacturing, transport and building construction, maintenance, and demolition. Increasing the necessity of reducing the environmental impact of buildings has led to enhancing not only the thermal performances of building materials but also the environmental sustainability of their production chains and waste prevention. As a consequence, novel thermo-insulating building materials or products have been developed by using both locally produced natural and waste/recycled materials that are able to provide good thermal performances while also having a lower environmental impact. In this context, the aim of this work is to provide a detailed analysis for the thermal characterization of recycled materials for building insulation. To this end, the thermal behavior of different materials representing industrial residual or wastes collected or recycled using Sardinian zero-km locally available raw materials was investigated, namely: (1) plasters with recycled materials; (2) plasters with natural fibers; and (3) building insulation materials with natural fibers. Results indicate that the investigated materials were able to improve not only the energy per-formances but also the environmental comfort in both new and in existing buildings. In particular, plasters and mortars with recycled materials and with natural fibers showed, respectively, values of thermal conductivity (at 20 °C) lower than 0.475 and 0.272 W/(mK), while that of building materials with natural fibers was always lower than 0.162 W/(mK) with lower values for com-pounds with recycled materials (0.107 W/(mK)). Further developments are underway to analyze the mechanical properties of these materials

Ventilation strategies to minimise the airborne virus transmission in indoor environments

A key challenge to fight the Covid-19 pandemic is to minimise the airborne transmission of the SARS-CoV-2 virus. Highly crowded indoor environments, such as schools, become possible hotspots for virus spreading because the basic non-pharmaceutical mitigation measures applied until now are not effective in reducing the virus airborne transmission mode, which is the principal one in indoor environments and requires improved ventilation. In the present study, a mass balance equation was applied to typical school scenarios to evaluate (i) required air exchange rates for mechanically-ventilated classrooms and (ii) adequate airing procedures for naturally ventilated classrooms. In the case of naturally ventilated classrooms, a feedback control strategy was evaluated using the measurements of indoor CO2. Our results show how these procedures can be applied in real life to support continued in-person instruction during a pandemic.publishedVersio

APPLICATION OF AHP TO INVENTORY MANAGEMENT AND COMPARISON TO CROSS ANALYSIS

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Covalent inactivation of Mycobacterium thermoresistibile inosine-5'-monophosphate dehydrogenase (IMPDH).

Inosine-5'-monophosphate dehydrogenase (IMPDH) is a rate-limiting enzyme involved in nucleotide biosynthesis. Because of its critical role in purine biosynthesis, IMPDH is a drug design target for immunosuppressive, anticancer, antiviral and antimicrobial chemotherapy. In this study, we use mass spectrometry and X-ray crystallography to show that the inhibitor 6-Cl-purine ribotide forms a covalent adduct with the Cys-341 residue of Mycobacterium thermoresistibile IMPDH

Lst4, the yeast Fnip1/2 orthologue, is a DENN-family protein.

The folliculin/Fnip complex has been demonstrated to play a crucial role in the mechanisms underlying Birt-Hogg-Dubé (BHD) syndrome, a rare inherited cancer syndrome. Lst4 has been previously proposed to be the Fnip1/2 orthologue in yeast and therefore a member of the DENN family. In order to confirm this, we solved the crystal structure of the N-terminal region of Lst4 from Kluyveromyces lactis and show it contains a longin domain, the first domain of the full DENN module. Furthermore, we demonstrate that Lst4 through its DENN domain interacts with Lst7, the yeast folliculin orthologue. Like its human counterpart, the Lst7/Lst4 complex relocates to the vacuolar membrane in response to nutrient starvation, most notably in carbon starvation. Finally, we express and purify the recombinant Lst7/Lst4 complex and show that it exists as a 1 : 1 heterodimer in solution. This work confirms the membership of Lst4 and the Fnip proteins in the DENN family, and provides a basis for using the Lst7/Lst4 complex to understand the molecular function of folliculin and its role in the pathogenesis of BHD syndrome.AP, BKB and RKN were supported by the Myrovlytis Trust. DBA was supported by a NHMRC CJ Martin Fellowship (APP1072476). LHW was supported by Medical Research Council (MRC) studentship, MR/J006580/1 and TPL by University College London. SD was supported by Fondation de France, La Ligue National contre le Cancer (Comité de Paris / Ile-de-France and Comité de l’Oise); TLB and NZ thank the University of Cambridge and The Wellcome Trust for facilities and support.This is the final version of the article. It was first available from Royal Society Publishing via http://dx.doi.org/10.1098/rsob.15017