Pursing of planar elastic pockets

The pursing of a simply- or doubly-connected planar elastic pocket by an applied pressure is analysed from the bending to the stretching regimes. The response is evaluated in terms of maximum deflection and profiles across a range of simply- and doubly-connected circular and square shapes. The study is conducted using experimental and numerical methods and supported by previous analytical results. The experimental method is based on an original 2D optical method that gives access to the pursing direction perpendicular to each image across the field of view. The equations for maximum pursing deflections are developed and compared for a range of thicknesses of silicone samples and shapes from the bending to the stretching regimes. In the case of doubly-connected shapes, dependence of maximum pursing deflection on clamped central circular and square areas or holes is quantified for both regimes. Good agreement is established between the three methods and the study also shows that the optical method may as well be successfully applied to problems of pursing of rubber pockets

Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome.

Acetaminophen overdose is the leading cause of acute liver failure. One dose of 10-15 g causes severe liver damage in humans, whereas repeated exposure to acetaminophen in humans and animal models results in autoprotection. Insight of this process is limited to select proteins implicated in acetaminophen toxicity and cellular defence. Here we investigate hepatic adaptation to acetaminophen toxicity from a whole proteome perspective, using quantitative mass spectrometry. In a rat model, we show the response to acetaminophen involves the expression of 30% of all proteins detected in the liver. Genetic ablation of a master regulator of cellular defence, NFE2L2, has little effect, suggesting redundancy in the regulation of adaptation. We show that adaptation to acetaminophen has a spatial component, involving a shift in regionalisation of CYP2E1, which may prevent toxicity thresholds being reached. These data reveal unexpected complexity and dynamic behaviour in the biological response to drug-induced liver injury

Historical culture and peace. How older generations address the need of younger generations to learn about theier in-group past

This chapter has three aims. First, it aims to disentangle social denial of in-group responsibilities for intergroup violence from other types of silence about intergroup violence. Secondly, it argues that intergenerational narratives which omit information about in-group responsibilities for violence that occurred before the birth of younger generations are highly risky to the descendants of perpetrators. Finally, it emphasises the importance of exploring in greater depth the understudied moment when a literal social denial about past in-group war crimes is exposed. To support these aims, the chapter presents results from a recent mixed methods, quasi-experimental study, which used between- and within-subject comparisons. The study asked young Italian university students to read an explicit text (“detailed text”) vs. a more nuanced one (“mild text”) about Italian war crimes occurring during the colonial invasion of Ethiopia. Data were collected before reading the text, during the reading and after it. Texts were constructed by manipulating the wording of a single historical narrative, taken from a textbook currently used in Italian high schools. The inclusion of this information in Italian history textbooks is quite recent, taking place approximately 70 years after the end of the war. Prior to this a widespread social denial silenced these crimes and as a result they were largely ignored in general social discourse. Results showed that participants reacted not only to the new information received but also to the way in which it was conveyed. The detailed narrative, by frankly taking a moral stance on past violence (a strategy that we named, after classic works of Foucault, 1983, parrhesia), provoked a better understanding of information, together with an increase of negative group-based moral emotions. Interestingly, while collective guilt did not differ between participants exposed to a detailed or a mild text, moral emotions distancing young participants from the responsibilities of older generations increased when these crimes were clearly exposed

Activating K-Ras mutations outwith "hotspot" codons in sporadic colorectal tumours:implications for personalised cancer medicine

The increasing burden of infectious diseases such as HIV, malaria and TB coupled with changing standards and regulatory requirements mean that many laboratory services in the world’s poorest countries are struggling to provide even a basic service. Chronic neglect has led to crippling staff shortages, inadequate funding, poor quality reagents and unreliable equipment. This situation is changing as international policy makers and funding agencies begin to recognise the critical role that laboratory services play in underpinning all aspects of healthcare. Many laboratory clinicians and scientists from the UK have experience of working and living in developing countries and have contributed to supporting their laboratory services. These efforts have generally not been widely disseminated, and there is no mechanism for learning lessons, for synergising or for avoiding duplication. Several recent government strategies urge UK institutions to increase their inputs to healthcare in poorer countries and there are several laboratory medicine initiatives (viz: the session on Pathology in the Developing World) that are putting this into practice. The RCPath is committed to playing a pivotal role in coordinating and expanding role of UK expertise in developing laboratory medicine in lowincome countries