31 research outputs found
Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573
XI ReuniĂŁo Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and BiotechnologyFibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding
intravascular thrombosis. In the pharmaceutical industry there is a search for new
fibrinolytic agent that reduces the production cost and increasing productivity. The
use of microorganism for enzyme production, such as the genus Streptomyces
has been reported. Streptomyces is a Gram-positive bacteria, responsible for
producing many bioactive compounds and extracellular enzymes of
pharmaceutical interest. This study aimed to evaluated the production of
fibrinolytic protease by Streptomyces sp DPUA 1573. Microbial cells were
cultivated in the ISP2 for 48 hours, after this period the strains were inoculated in
MS2 (soybean medium) that according with factorial design 24 (concentrations of
soybean 0.5; 1.0 and 1.5%, glucose 0; 0.5 and 1.0% and different speeds 150
rpm; 200 rpm and 250 rpm and temperature 28C; 30C and 32C). The factorial
design was analyzed by variance analysis (anova) and the glucose concentration
showed a positive and significative effect. The results showed that the variable
interaction had significant effect. that the best condition was composed 1.5%
soybean, 1% glucose, 28 ÂșC and 150 speed in 48 hours, with production
fibrinolytic 1391.66 U/mL. These values were higher than those reported in the
literature. However these results show the biggest potencial in production
fibrinolytic enzyme by Streptomyces.info:eu-repo/semantics/publishedVersio
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology
Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2ÂČ central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study.Brazilian Research Funding InstitutionsCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES
Assessment of toxicity of a biosurfactant from Candida sphaerica UCP 0995 cultivated with industrial residues in a bioreactor
Background: The aim of the present study was to propose a low-cost
method for the production of a biosurfactant by the yeast Candida
sphaerica and assess its toxicity and phytotoxicity. The medium was
formulated with distilled water supplemented with residue from a soy
oil refinery (5%) and corn steep liquor (2.5%) as substrates. These two
products were the sources of carbon and nitrogen as well as mineral
elements to encourage the growth of the microorganism and production of
a biosurfactant. Results: The isolated biosurfactant yield was 6.364
g/l. The biosurfactant exhibited an excellent ability to reduce surface
tension (26 mN/m) and demonstrated no toxicity against seeds of
Brassica oleracea , Chicoria intybus and Solanum gilo or the micro
crustacean Artemia salina employed as a bioindicator. The
biosurfactant exhibited no antimicrobial activity against the fungi and
bacteria tested. Conclusions: The promising results obtained in this
study indicate the feasibility of producing biosurfactants from
powerful non-toxic organic residues and their application in the
bioremediation of contaminated soil and water
Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens
Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm2. Three variables at two levels were used to assess their effects on the fibrinolytic production. The parameters studied were agitation (0.28 - 1.12 g), temperature (28 - 36 ÂșC) and pH (6.0 - 8.0); all of them had significant effects on the fibrinolytic production. The maximum fibrinolytic activity (304 mm2) was observed at 1.12 g, 28 ÂșC, and pH of 8.0. The crude extract of the fermentation broth was used to assess the biochemical properties of the enzyme. Protease and fibrinolytic activities were stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively. Optimum temperature for protease activity ranged between 35 and 55 °C, while the highest fibrinolytic activity was observed at 45 ÂșC. Proteolytic activity was inhibited by Cu2+ and Co2+ ions, phenylmethylsulfonyl fluoride (PMSF) and pepstatin A, which suggests that the enzyme is a serine protease. Enzymatic extract cleaved fibrinogen at the subunits A-chain, A-chain, and -chain. The results indicated that Streptomyces sp. DPUA 1576 produces enzymes with fibrinolytic and fibrinogenolytic activity, enzymes with an important application in the pharmaceutical industry.The authors grateful acknowledge the financial support of Fundação de Amparo a Pesquisa do Estado de Pernambuco (FACEPE, Pernambuco, Brazil, N. 0158-2.12/11), CNPq/ RENORBIO (National Counsel of Technological and Scientific Development, N.55146/2010-3) and National Council for the Improvement of Higher Education (CAPES, Brazil) for the scholarship. The author thanks editor and reviewers for their review and comments.info:eu-repo/semantics/publishedVersio
Life experiences of patients who have completed tuberculosis treatment: a qualitative investigation in southeast Brazil
Novas evidĂȘncias documentais para a histĂłria da homeopatia na AmĂ©rica Latina: um estudo de caso sobre os vĂnculos entre Rio de Janeiro e Buenos Aires
Sele\ue7\ue3o de leveduras da Regi\ue3o Amaz\uf4nica para produ\ue7\ue3o de protease extracelular
Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems
PEG-citrate Aqueous Two-Phase Systems (ATPS) were used to recover and partially purify protease from Aspergillus tamarii URM4634 produced by Solid State Fermentation. Experiments were performed according to a 24-full factorial design using PEG molar mass (MPEG), PEG concentration (CPEG), citrate concentration (CCIT) and pH as independent variables; and purification factor (PF), partition coefficient (K) and activity yield (Y) as responses. Protease showed high activity in the PEG-rich phase, also MPEG and CCIT were shown to exert positive effects on all responses. The highest purification factor (3.95) was obtained using MPEG=8000 g/mol, 24% (w/w) CPEG, 20% (w/w) CCIT at pH 8.0. Consequently, the selected ATPS proved to be efficient and can be used as a first step for pre-purification of protease from solid state fermented of A. tamarii URM4634