XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and BiotechnologyFibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding
intravascular thrombosis. In the pharmaceutical industry there is a search for new
fibrinolytic agent that reduces the production cost and increasing productivity. The
use of microorganism for enzyme production, such as the genus Streptomyces
has been reported. Streptomyces is a Gram-positive bacteria, responsible for
producing many bioactive compounds and extracellular enzymes of
pharmaceutical interest. This study aimed to evaluated the production of
fibrinolytic protease by Streptomyces sp DPUA 1573. Microbial cells were
cultivated in the ISP2 for 48 hours, after this period the strains were inoculated in
MS2 (soybean medium) that according with factorial design 24 (concentrations of
soybean 0.5; 1.0 and 1.5%, glucose 0; 0.5 and 1.0% and different speeds 150
rpm; 200 rpm and 250 rpm and temperature 28C; 30C and 32C). The factorial
design was analyzed by variance analysis (anova) and the glucose concentration
showed a positive and significative effect. The results showed that the variable
interaction had significant effect. that the best condition was composed 1.5%
soybean, 1% glucose, 28 ºC and 150 speed in 48 hours, with production
fibrinolytic 1391.66 U/mL. These values were higher than those reported in the
literature. However these results show the biggest potencial in production
fibrinolytic enzyme by Streptomyces.info:eu-repo/semantics/publishedVersio
