A PRND polymorphism in Churra do Campo portuguese sheep breed

Prion-like Doppel gene (PRND) is located downstream from priori protein gene (PRNP). Doppel protein is not related to prion disease but to male fertility. Our previous analysis of PRND coding region in 460 animals from 8 Portuguese sheep breeds, by multiple restriction fragment-single strand conformation polymorphism (MRFSSCP), revealed a synonymous substitution (78G>A). An association was found between identified PRND polymorphism and PRNP genotypes, determined by primer extension and grouped into 5 grades of increasing scrapie susceptibility-R1 to R5: PRND was monomorphic (GG) in animals with most resistant ARR/ARR PRNP genotype-R1; higher frequency of heterozygotes (GA) was significantly associated with ARQ/AHQR4. Therefore, EU selection programme to eradicate scrapie in sheep, based on PRNP genotypes, may reduce genetic diversity, with hypothetical repercussions on reproduction. The aim of current work was to evaluate 78G>A PRND polymorphism in highly endangered Churra do Campo Portuguese sheep breed. From a total of 73 animals analysed (16 R1, 36 R3, 18 R4, 3 R5), 72 were GG and 1 GA, the later being ARQ/ARQ (R4). Low incidence of PRND polymorphic variants in this breed may be explained by mating involving small number of related animals, and particular differences in distribution of PRNP genotypes

Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca Linnaeus, 1766) criada em cativeiro

Considerando-se a falta de informações detalhadas sobre a morfologia da paca, sendo o segundo maior roedor da fauna brasileira, de excelente qualidade de carne e, dada a importância do tegumento comum, inclusive para o bom manejo em cativeiro, e até mesmo estudos como uma opção de animal de experimentação, descreveu-se a morfologia, morfometria e a ultraestrutura da pele de oito pacas (Cuniculus paca) machos e fêmeas, mediante a análise comparativa de segmentos cutâneos das regiões cervical, dorsal e medial do carpo. Observaram-se macroscopicamente as características da pelagem. Parte dos segmentos das regiões cutâneas foi analisada à microscopia de luz e parte, à microscopia eletrônica de varredura. Mensuraram-se as espessuras da derme, epiderme, camada córnea, perfis das fibras de colágeno da derme reticular e a área dos perfis das células das glândulas sebáceas repletas. Analisaram-se os resultados pela estatística descritiva e teste "T" (p<0,001). A coloração da pelagem da paca é castanho avermelhado com cerdas organizadas em grupos. A arquitetura da cútis e os anexos cutâneos se assemelham aos dos mamíferos em geral, embora haja ausência de glândulas sudoríparas. De acordo com a analise morfométrica, pôde-se inferir que a arquitetura da cútis de pacas machos e fêmeas apresenta diferenças quando comparada entre os sexos e também em um mesmo animal, diferenciando-se entre diversas regiões corpóreas

A Study Of V2o5-k2so4-sio2 Catalysts For Catalytic Vapor-phase Oxidation Of Toluene To Benzaldehyde

The vapor phase catalytic oxidation of toluene to benzaldehyde has been studied over V2O5-K2SO4-SiO2 catalysts in an isothermal differential reactor. The experiments were carried out at atmospheric pressure, temperatures from 410°C to 470°C and the modified spatial time (W/FT0) ranging from 0 to 180 g cat/mol toluene/h. The experimental tests showed the best performance for the catalyst obtained by co-precipitation. These results may be due to a crystalline phase identified in the process of catalyst characterization. Reaction kinetics was determined using the Mars and van Krevelen model.11011931201Haber, (1992) New Developments in Selective Oxidation by Heterogeneous Catalysis, , Amsterdam, ElsevierTrimm, D.L., Irshad, M., (1970) Journal of Catalysis, 18, p. 142Papadatos, K., Shelstad, K.A., (1973) Journal of Catalysis, 28, p. 116Van Hengstum, A.J., Van Ommen, J.G., Bosch, H., Gellings, P.J., (1983) Applied Catalysis, 8, p. 369Günduz, G., Akpolat, O., (1990) Ind. Eng. Chem. Res., 29, p. 45Ray, S.K., Mukherjee, P.N., (1983) Indian Journal of Technology, 21, p. 137Satterfield, Sherwood, T.K., (1963) The Role of Diffusion in Catalysis, , Addison-Wesley Publishing Company, IncMars, Van Krevelen, D.W., (1954) Chem. Eng. Sci. Suppl., 3, p. 41Marquardt, (1963) Soc. Ind. Appl. Math. J., 11Stiles, A.B., (1987) Catalyst Supports and Supported Catalysts, , Butterworth Publishers, United States of Americ