The post-translational methylation of arginine in the glycine arginine rich region of CHO nucleoin

Nucleolin is a nucleolar protein important for ribosome biogenesis. Nucleolin contains a conserved glycine arginine rich (GAR) domain near its carboxy terminus. GAR domains are defined by repeating tri-peptide, (arginine -glycine-glycine (RGG)) motifs. The arginine in the RGG motif is post-translationally modified by dimethylation on one of the two guanido nitrogens. Although arginine methylation was identified over 30 years ago, the function of this modification remains unknown. The GAR domain of nucleolin is important for nucleic acid binding and for nucleolar localization of nucleolin. This dissertation describes investigates possible in vivo interactions between nucleolin and other nucleolar proteins involved in ribosome biogenesis. This dissertation also addresses the possible roles that arginine dimethylation may play in the function of the GAR domain. A GAR truncated hamster nucleolin (DGAR) localizes to the nucleoplasm and not to the nucleoli of CHO cells. This version of nucleolin was subsequently used to probe the in vivo interactions of nucleolin with other non-ribosomal nucleolar proteins. In support of previous work, DGAR caused endogenous B23 to redistribute to the nucleoplasm, suggesting an in vivo interaction. Endogenous Nopp140 shows no redistribution in the presence of DGAR. The effect of DGAR on the nucleolar protein, fibrillarin, remains unclear. A redistribution of exogenously expressed wild type nucleolin from nucleoli to the nucleoplasm is observed when CHO cells are treated with the methyltransferase inhibitors 5-methyl 5¢ deoxythioadenosine (MTA) and 3-deaza-adenosine (DAA). This redistribution of nucleolin is reversible and independent of protein synthesis. The arginines in the RGG motifs of the GAR domain of hamster nucleolin were changed to lysine, and the fully substituted protein was not a substrate for the RGG specific protein arginine methyltransferase, Hmt1p. The lysine substituted protein bound nucleic acids and behaved in vivo in a manner indistinguishable from wild type nucleolin. These results indicate that methylation is not necessary for in vitro nucleic acid binding or for in vivo localization of nucleolin, and that the redistribution of nucleolin observed following MTA treatment is likely due to inhibiting the methylation of another nucleolar substrate

Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions

There is extensive data to support the use of commercial transport media as a stabilizer for known clinical samples; however, there is little information to support their use outside of controlled conditions specified by the manufacturer. Furthermore, there is no data to determine the suitability of said media for biological pathogens, specifically those of interest to the US military. This study evaluates commercial off-the-shelf (COTS) transport media based on sample recovery, viability, and quality of nucleic acids and peptides for nonpathogenic strains of Bacillus anthracis, Yersinia pestis, and Venezuelan equine encephalitis virus, in addition to ricin toxin. Samples were stored in COTS, PBST, or no media at various temperatures over an extended test period. The results demonstrate that COTS media, although sufficient for the preservation of nucleic acid and proteinaceous material, are not capable of maintaining an accurate representation of biothreat agents at the time of collection

The style of the new objectivity in the early poetical work of Erich Kästner and literary science theories of the new objectivity

Práce zkoumá, zda raná básnická tvorba Ericha Kästnera, jež bývá označována označováno za vzorový příklad tvorby německé nové věcnosti, skutečně splňuje příznaky nové věcnosti ve smyslu (raných) teorií, které novou věcnost jako literární směr definovaly. Tedy zda jsou rané, případně i pozdější literární teorie nové věcnosti skutečně adekvátní pro konkrétní literární produkci jednoho autora, která je produkcí nové věcnosti běžně nazývána.This study examines whether the early poetic production of Erich Kästner , which used to be designated a model example of the production of the German New Objectivity, really fulfils the characteristics of the New Objectivity in the sense of the theories defining the New Objectivity as a literary movement. The question is whether the early and later literary theories of the New Objectivity are really adequate for describing the actual literary production of one author, commonly designated as a product of New Objectivity.Institute of Germanic StudiesÚstav germánských studiíFilozofická fakultaFaculty of Art

The style of the new objectivity in the early poetical work of Erich Kästner and literary science theories of the new objectivity

Práce zkoumá, zda raná básnická tvorba Ericha Kästnera, jež bývá označována označováno za vzorový příklad tvorby německé nové věcnosti, skutečně splňuje příznaky nové věcnosti ve smyslu (raných) teorií, které novou věcnost jako literární směr definovaly. Tedy zda jsou rané, případně i pozdější literární teorie nové věcnosti skutečně adekvátní pro konkrétní literární produkci jednoho autora, která je produkcí nové věcnosti běžně nazývána.This study examines whether the early poetic production of Erich Kästner , which used to be designated a model example of the production of the German New Objectivity, really fulfils the characteristics of the New Objectivity in the sense of the theories defining the New Objectivity as a literary movement. The question is whether the early and later literary theories of the New Objectivity are really adequate for describing the actual literary production of one author, commonly designated as a product of New Objectivity.Institute of Germanic StudiesÚstav germánských studiíFilozofická fakultaFaculty of Art

Úloha a použití řečových aktů v dialozích románu Pýcha a předsudek Jane Austenové

This work from the field of pragmatics introduces the application of the concept of speech acts (see J. L. Austin, J. Searle) to the literary sample of 15 chosen dialogues i.e. 1122 sentences from the novel Pride and Prejudice by the classical English author Jane Austen. It introduces an eight-member modified classification of speech acts: representatives, assertives, directives, connissives, expressives, interrogatives, requestives and daclarations. There are eight literary characters included in the research together with marginally Charlotte Lucas, who use speech acts to express their communicative intentions. The main heroine Elizabeth occurring in 12 dialogues uses mostly representatives, assertives and expressives. The remaining three dialogues involve Mrs Bennet and her husband Mr Bennet. Jane Austen's language is very rich and complex, with frequent occurrence of politeness turns of phrase. Some mixed and multiple categories also add to this complexity (there are 55.8% of simple ones; 39.1% of double, 4.6% of triple, quadruple only 0.5 % of the 969 sentences counted). This work also contains some comments on stylistic analysis featuring selected interesting literary and pragmatic aspects of the dialogical samples.Tato práce z oblasti pragmatiky přináší aplikaci konceptu řečových aktů (viz např. J. L. Austin, J. Searle) na literární vzorek 15 vybraných dialogů o celkovém rozsahu 1122 vět z románu Pýcha a předsudek klasické anglické autorky Jane Austenové. Představena je osmičlenná modifikace klasifikace řečových aktů: representativa, assertiva, direktiva, commissiva, expressiva, interrogativa, requestiva a deklarativa. Do výzkumu je započítáno celkem osm literárních postav (a dále okrajově Charlotta Lucasová), které používají řečové akty k vyjadřování svých komunikačních záměrů. Výzkum ukazuje, že nejčetněji se vyskytují representativa, expressiva a assertiva, zatímco okrajová jsou direktiva. Hlavní hrdinka Elizabeth, zastoupená ve 12 dialozích, nejvíce používá representativa, assertiva a expressiva. Zbývající 3 dialogy vede paní Bennetová se svým manželem panem Bennetem. Jazyk Austenové je celkově velmi bohatý a komplexní, s častým výskytem zdvořilostních formulací. Tomu odpovídají také smíšené, vícečetné kategorie (jednoduchých je 55,8; dvojitých 39,1%, trojitých 4,6 %, čtverných pouze 0,5% z počítaných 969 vět). Práce navíc obsahuje komentáře stylistického rozboru s vybranými zajímavými literárními i doplňujícími pragmatickými aspekty zkoumaných dialogických ukázek.Katedra anglického jazyka a literaturyPedagogická fakultaFaculty of Educatio

Plasma Dynamics

Contains reports on three research projects.National Science Foundation (Grant G-9330)United States Air Force, Air Force Cambridge Research Center (Contract AF19(604)-5992)United States ArmyUnited States Air Force (Contract AF19(604)-7400)Lincoln Laboratory (Purchase Order B-00306)Flight Accessories Laboratory, Wright-Patterson Air Force Base (WADD Contract AF33(616)-7624)United States Air Force, Air Force Cambridge Research Laboratories (Contract AF19(604)-4551)United States NavyUnited States Atomic Energy Commission (Contract AT(30-1)-1842