Genetic variation among lambs in peripheral IgE activity against the larval stages of <i>Teladorsagia circumcincta</i>

IgA and IgE activity against Teladorsagia circumcincta was investigated in a flock of Texel lambs following natural, mixed nematode infection among lambs. The distribution of IgA activity was similar to a gamma distribution whereas IgE activity was different. Box-Cox analysis demonstrated that X0.25 was a suitable transformation to normalise IgE responses. The transformed IgE activity was under moderate to strong genetic control. Nine different allergens were identified by proteomic analysis. Tropomyosin was selected for further analysis. IgE activity against tropomyosin was moderately heritable and associated with decreased egg counts and with reduced body weight at the time of sampling

A study of cultivation and sowing systems for cereals.

End of Project ReportDue to the pressure on cereal margins growers are seeking ways of reducing their costs of production. Reduced cultivation may provide cheaper and faster crop establishment, but in the past has been subject to problems with (poor crop establishment) weeds and soil compaction. With modern cultivation and sowing technology and methodology it may be possible to overcome these difficulties. In addition reduced cultivation is considered to be more environmentally friendly and sustainable than the conventional plough-based system. With these considerations in mind it was decided to start an experiment in autumn 2000 to compare the conventional system (PL) with reduced cultivation (RC) for crop establishment, and to examine the effect of incorporating the straw into then soil or baling and removing it. The treatments were assessed on winter wheat, and winter and spring barley. The parameters assessed included – crop establishment, yield and quality effects on disease levels, invertebrate pests and beneficials (aphids, slugs, earthworms, beetles), and soil conditions. The work reported here was conducted on four trial sites at Oak Park, Carlow and Knockbeg, Co. Laois from 2000 to 2003. the objectives were to establish the efficacy of RC compared with PL and to its effects on the parameters outlined above. Plant establishment was lower after RC in most of the experiments. Winter wheat yields were good irrespective of the number of plants established and there were no significant differences between cultivation or straw disposal method. The relative yields of the PL and RC treatments in winter barley varied over the three years; in 2001 there was no difference, in 2002 PL yielded 1 t/ha more than RC, while in 2003 the PL area produced 2 t/ha extra. Spring barley yields were similar on the PL and RC treatments. Broadleaved weeds were not a problem on the PL or RC treatments but there were more annual grass weeds on the RC plots. This was particularly serious in the winter barley experiments where sterile brome (Bromus sterilis) had a big effect on yield by 2003. annual meadow grass (Poa annua) was a problem in both PL and RC areas if not controlled by timely herbicide application; this was particularly so on the headland areas in the spring barley field. Disease assessment on the winter wheat trail showed no significant differences in Take-all or Eyespot levels between cultivation or straw disposal methods, although Take-all levels tended to be lower on the RC plots. Soil strength as measured by cone penetrometer and shear vane was higher on the RC treatments. In the winter wheat there was no difference in slug numbers between any of the treatments. Leaf damage by slugs increased in RC relative to PL but not significantly, in 2003. No below ground slug damage was found. Earthworm numbers in the winter barley increased significantly on the RC treatment relative to the PL as the study progressed. Straw incorporation increased earthworm numbers on both PL and RC treatments. Allolobophora chlorotia was the most common species in 2004. In the winter wheat the RC and straw incorporation increased earthworm numbers. Cultivation method had a greater effect on ground beetle numbers than method of straw disposal. Large species (e.g. Pterostichies malanarius) were favoured by RC while smaller species (e.g. Bembidon species) were more numerous in the PL plots. Molecular studies on virus diseases, vectors and vector-predators are underway in progress. In the invertebrate pests and beneficials investigations the RC winter barley had 11% fewer aphids and 27% less BYDV than the PL. Incorporating straw reduced aphid numbers and virus incidence by 36% and 32% respectively; the comparable values for the PL + straw treatment were 15% and 15%. In the winter wheat there was less BYDV in the RC plots than the PL + less were the straw was incorporated than where the straw was removed. Aphid infestation of wheat ears was low in the three years of the experiment. The insecticide seed treatment imidacloprid significantly reduced aphid numbers and virus incidence in winter barley but was less effective than a single insecticide spray in controlling the disease. The seed treatment was more effective in controlling BYDV in RC than in the PL plots. Estimations of slug numbers in the winter barley showed that the dominant species was the grey field slug (Deroceras reticulatum). Slug numbers increased significantly on all treatments between 2001 and 2004. In November 2004, slugs were significantly more numerous in the RC treatments than in the PL areas. Leaf damage was proportional to slug populations, but the slugs did not damage the barely seed or reduce plant populations in continuous winter barley under RC. Root and stem diseases (Take-all and Eyespot) were not nay worse under RC than after PL. In fact there was a tendency towards lower disease levels on the RC areas and there was significantly less sharp eyespot on the RC treatment

Optical requirements and modelling of coupling devices for the SAFARI instrument on SPICA

The next generation of space missions targeting far-infrared bands will require large-format arrays of extremely low-noise detectors. The development of Transition Edge Sensors (TES) array technology seems to be a viable solution for future mm-wave to Far-Infrared (FIR) space applications where low noise and high sensitivity is required. In this paper we concentrate on a key element for a high sensitivity TES detector array, that of the optical coupling between the incoming electromagnetic field and the phonon system of the suspended membrane. An intermediate solution between free space coupling and a single moded horn is where over-moded light pipes are used to concentrate energy onto multi-moded absorbers. We present a comparison of modelling techniques to analyse the optical efficiency of such light pipes and their interaction with the front end optics and detector cavity

Observable Effects of Scalar Fields and Varying Constants

We show by using the method of matched asymptotic expansions that a sufficient condition can be derived which determines when a local experiment will detect the cosmological variation of a scalar field which is driving the spacetime variation of a supposed constant of Nature. We extend our earlier analyses of this problem by including the possibility that the local region is undergoing collapse inside a virialised structure, like a galaxy or galaxy cluster. We show by direct calculation that the sufficient condition is met to high precision in our own local region and we can therefore legitimately use local observations to place constraints upon the variation of "constants" of Nature on cosmological scales.Comment: Invited Festscrift Articl

The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3)

Salmonid alphavirus is the aetological agent of pancreas disease (PD) in marine Atlantic salmon, Salmo salar, and rainbow trout, Oncorhynchus mykiss, with most outbreaks in Norway caused by SAV subtype 3 (SAV3). This atypical alphavirus is transmitted horizontally causing a significant economic impact on the aquaculture industry. This histopathological and proteomic study, using an established cohabitational experimental model, investigated the correlation between tissue damage during PD and a number of serum proteins associated with these pathologies in Atlantic salmon. The proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting. A number of humoral components of immunity which may act as biomarkers of the disease were also identified. For example, creatine kinase, enolase and malate dehydrogenase serum concentrations were shown to correlate with pathology during PD. In contrast, hemopexin, transferrin, and apolipoprotein, amongst others, altered during later stages of the disease and did not correlate with tissue pathologies. This approach has given new insight into not only PD but also fish disease as a whole, by characterisation of the protein response to infection, through pathological processes to tissue recovery. Biological significance: Salmonid alphavirus causes pancreas disease (PD) in Atlantic salmon, Salmo salar, and has a major economic impact on the aquaculture industry. A proteomic investigation of the change to the serum proteome during PD has been made with an established experimental model of the disease. Serum proteins were identified by two-dimensional electrophoresis, trypsin digest and peptide MS/MS fingerprinting with 72 protein spots being shown to alter significantly over the 12 week period of the infection. The concentrations of certain proteins in serum such as creatine kinase, enolase and malate dehydrogenase were shown to correlate with tissue pathology while other proteins such as hemopexin, transferrin, and apolipoprotein, altered in concentration during later stages of the disease and did not correlate with tissue pathologies. The protein response to infection may be used to monitor disease progression and enhance understanding of the pathology of PD

Signatures of quantum effects on radiation reaction in laser-electron-beam collisions

Two signatures of quantum effects on radiation reaction in the collision of a GeV electron beam with a high intensity (>3×1020Wcm-2]]>) laser pulse have been considered. We show that the decrease in the average energy of the electron beam may be used to measure the Gaunt factor for synchrotron emission. We derive an equation for the evolution of the variance in the energy of the electron beam in the quantum regime, i.e. quantum efficiency parameter . We show that the evolution of the variance may be used as a direct measure of the quantum stochasticity of the radiation reaction and determine the parameter regime where this is observable. For example, stochastic emission results in a 25% increase in the standard deviation of the energy spectrum of a GeV electron beam, 1 fs after it collides with a laser pulse of intensity 1021W cm-2. This effect should therefore be measurable using current high-intensity laser systems

Antihydrogen formation dynamics in a multipolar neutral anti-atom trap

Antihydrogen production in a neutral atom trap formed by an octupole-based magnetic field minimum is demonstrated using field-ionization of weakly bound anti-atoms. Using our unique annihilation imaging detector, we correlate antihydrogen detection by imaging and by field-ionization for the first time. We further establish how field-ionization causes radial redistribution of the antiprotons during antihydrogen formation and use this effect for the first simultaneous measurements of strongly and weakly bound antihydrogen atoms. Distinguishing between these provides critical information needed in the process of optimizing for trappable antihydrogen. These observations are of crucial importance to the ultimate goal of performing CPT tests involving antihydrogen, which likely depends upon trapping the anti-atom

Novel methods of targeting IL-1 signalling for the treatment of breast cancer bone metastasis

Breast cancer bone metastasis is currently incurable. Evidence suggests that inhibiting IL-1 signalling with the IL1R antagonist, Anakinra, or the IL1β antibody, Canakinumab, prevents metastasis and almost eliminates breast cancer growth in the bone. However, these drugs increase primary tumour growth. We, therefore, investigated whether targeting other members of the IL-1 pathway (Caspase-1, IL1β or IRAK1) could reduce bone metastases without increasing tumour growth outside of the bone. Inhibition of IL-1 via MLX01 (IL1β secretion inhibitor), VRT043198/VX765 (Caspase-1 inhibitor), Pacritinib (IRAK1 inhibitor) or Anakinra (IL1R antagonist) on tumour cell viability, migration and invasion were assessed in mouse mammary E0771 and Py8119 cells in vitro and on primary tumour growth, spontaneous metastasis and metastatic outgrowth in vivo. In vitro, Inhibition of IL-1 signalling by MLX01, VRT043198 and Anakinra reduced migration of E0771 and Py8119 cells and reversed tumour-derived IL1β induced-increased invasion and migration towards bone cells. In vivo, VX765 and Anakinra significantly reduced spontaneous metastasis and metastatic outgrowth in the bone, whereas MLX01 reduced primary tumour growth and bone metastasis. Pacritinib had no effect on metastasis in vitro or in vivo. Targeting IL-1 signalling with small molecule inhibitors may provide a new therapeutic strategy for breast cancer bone metastasis

Search For Trapped Antihydrogen

We present the results of an experiment to search for trapped antihydrogen atoms with the ALPHA antihydrogen trap at the CERN Antiproton Decelerator. Sensitive diagnostics of the temperatures, sizes, and densities of the trapped antiproton and positron plasmas have been developed, which in turn permitted development of techniques to precisely and reproducibly control the initial experimental parameters. The use of a position-sensitive annihilation vertex detector, together with the capability of controllably quenching the superconducting magnetic minimum trap, enabled us to carry out a high-sensitivity and low-background search for trapped synthesised antihydrogen atoms. We aim to identify the annihilations of antihydrogen atoms held for at least 130 ms in the trap before being released over ~30 ms. After a three-week experimental run in 2009 involving mixing of 10^7 antiprotons with 1.3 10^9 positrons to produce 6 10^5 antihydrogen atoms, we have identified six antiproton annihilation events that are consistent with the release of trapped antihydrogen. The cosmic ray background, estimated to contribute 0.14 counts, is incompatible with this observation at a significance of 5.6 sigma. Extensive simulations predict that an alternative source of annihilations, the escape of mirror-trapped antiprotons, is highly unlikely, though this possibility has not yet been ruled out experimentally.Comment: 12 pages, 7 figure

Effect of laser temporal intensity skew on enhancing pair production in laser - Electron-beam collisions

Recent high-intensity laser experiments (Cole et al 2018 Phys. Rev. X 8 011020; Poder et al 2018 Phys. Rev. X 8 031004) have shown evidence of strong radiation reaction in the quantum regime. Experimental evidence of quantum effects on radiation reaction and electron-positron pair cascades has, however, proven challenging to obtain and crucially depends on maximising the quantum parameter of the electron (defined as the ratio of the electric field it feels in its rest frame to the Schwinger field). The quantum parameter can be suppressed as the electrons lose energy by radiation reaction as they traverse the initial rise in the laser intensity. As a result the shape of the intensity temporal envelope becomes important in enhancing quantum radiation reaction effects and pair cascades. Here we show that a realistic laser pulse with a faster rise time on the leading edge, achieved by skewing the temporal envelope, results in curtailing of pair yields as the peak power is reduced. We find a reduction in pair yields by orders of magnitude in contrast to only small reductions reported previously in large-scale particle-in-cell code simulations (Hojbota et al 2018 Plasma Phys. Control. Fusion 60 064004). Maximum pairs per electron are found in colliding 1.5 GeV electrons with a laser wakefield produced envelope 7.90 × 10-2 followed by a short 50 fs Gaussian envelope, 1.90 × 10-2, while it is reduced to 8.90 × 10-5, a factor of 100, for an asymmetric envelope