Two binding partners cooperate to activate the molecular motor Kinesin-1

The regulation of molecular motors is an important cellular problem, as motility in the absence of cargo results in futile adenosine triphosphate hydrolysis. When not transporting cargo, the microtubule (MT)-based motor Kinesin-1 is kept inactive as a result of a folded conformation that allows autoinhibition of the N-terminal motor by the C-terminal tail. The simplest model of Kinesin-1 activation posits that cargo binding to nonmotor regions relieves autoinhibition. In this study, we show that binding of the c-Jun N-terminal kinase–interacting protein 1 (JIP1) cargo protein is not sufficient to activate Kinesin-1. Because two regions of the Kinesin-1 tail are required for autoinhibition, we searched for a second molecule that contributes to activation of the motor. We identified fasciculation and elongation protein ζ1 (FEZ1) as a binding partner of kinesin heavy chain. We show that binding of JIP1 and FEZ1 to Kinesin-1 is sufficient to activate the motor for MT binding and motility. These results provide the first demonstration of the activation of a MT-based motor by cellular binding partners

Hysteresis and the dynamic phase transition in thin ferromagnetic films

Hysteresis and the non-equilibrium dynamic phase transition in thin magnetic films subject to an oscillatory external field have been studied by Monte Carlo simulation. The model under investigation is a classical Heisenberg spin system with a bilinear exchange anisotropy in a planar thin film geometry with competing surface fields. The film exhibits a non-equilibrium phase transition between dynamically ordered and dynamically disordered phases characterized by a critical temperature Tcd, whose location of is determined by the amplitude H0 and frequency w of the applied oscillatory field. In the presence of competing surface fields the critical temperature of the ferromagnetic-paramagnetic transition for the film is suppressed from the bulk system value, Tc, to the interface localization-delocalization temperature Tci. The simulations show that in general Tcd < Tci for the model film. The profile of the time-dependent layer magnetization across the film shows that the dynamically ordered and dynamically disordered phases coexist within the film for T < Tcd. In the presence of competing surface fields, the dynamically ordered phase is localized at one surface of the film.Comment: PDF file, 21 pages including 8 figure pages; added references,typos added; to be published in PR

Anterior-inferior plating of middle-third fractures of the clavicle

© The Author(s) 2009. This article is published with open access at Springerlink.com Introduction Various techniques have been reported for the treatment of middle-third clavicle (collar bone) fractures. This prospective study was conducted to evaluate the results of anterior-inferior plating using a 3.5-mm reconstruction plate for the treatment of middle-third clavicle fractures

Graphite foam for cooling of automotive power electronics

Abstract -Hybrid and fuel cell vehicles utilize the Si-based IGBT (Integrated Gate Bipolar Transistor) controller which must dissipate about 100 W/cm 2 heat and maintain a temperature below 125°C. The application of porous, high thermal conductivity carbon foam, a new class of advanced lightweight material, to the thermal management of this electronic system and the use of micro-and nano-scale thermal measurement methods for analyzing thermal transport in electronics are presented. Development of advanced carbon foam with different pore structure by variation of the foaming pressure is discussed. The use of carbon foam to remove the heat generated in power electronics has been studied in three approaches: 1) forced air convection, 2) water cooled heat exchanger, and 3) thermosyphoning

Sensitivities of the NCEP Global Forecast System

An important issue in developing a forecast system is its sensitivity to additional observations for improving initial conditions, to the data assimilation (DA) method used, and to improvements in the forecast model. These sensitivities are investigated here for the Global Forecast System (GFS) of the National Centers for Environmental Prediction (NCEP). Four parallel sets of 7-day ensemble forecasts were generated for 100 forecast cases in mid-January to mid-March 2016. The sets differed in their 1) inclusion or exclusion of additional observations collected over the eastern Pacific during the El Ni&ntilde;o Rapid Response (ENRR) field campaign, 2) use of a hybrid 4D&ndash;EnVar versus a pure EnKF DA method to prepare the initial conditions, and 3) inclusion or exclusion of stochastic parameterizations in the forecast model. The Control forecast set used the ENRR observations, hybrid DA, and stochastic parameterizations. Errors of the ensemble-mean forecasts in this Control set were compared with those in the other sets, with emphasis on the upper-tropospheric geopotential heights and vorticity, midtropospheric vertical velocity, column-integrated precipitable water, near-surface air temperature, and surface precipitation. In general, the forecast errors were found to be only slightly sensitive to the additional ENRR observations, more sensitive to the DA methods, and most sensitive to the inclusion of stochastic parameterizations in the model, which reduced errors globally in all the variables considered except geopotential heights in the tropical upper troposphere. The reduction in precipitation errors, determined with respect to two independent observational datasets, was particularly striking.</p

Comparative analysis of eating quality and yield of selected non-waxy red-pericarp aromatic rice mutants

Red-pericarp variety Kuanfu waxy aroma is highly valued for its grain quality in Taiwan, but it has undesirable traits of awned rough grain and taller plant height.  The present study compared the palatability of cooked rice grains and yields of Kuanfu waxy aroma and its ten NaN3-induced awnless non-waxy aromatic M6-generation mutants developed through single-seed-descent selection (from M2 to M6 generation) plus a non-waxy aromatic rice variety TNG71 (reference variety with good eating quality).  The palatability of cooked rice grains was assessed by using a rice taste meter.  Results indicated that all the mutants exhibited awnless grain traits and reduced plant height.  PCR analyses confirmed the expression of fragrance (fgr) gene in these mutants.  Significant differences in the palatability of cooked rice were also observed among the mutants with AM-425 (70.45) and AM-430 (73.75) having higher palatability scores than TNG71 (69.32).  Mutant AM-425 also had higher aroma sensory score (1.33) than TNG71 (1.17).  Two years yield trials indicated that AM-425 and AM-430 significantly out-yielded Kuanfu waxy aroma and can be recommended to rice growers.

Lack of complex I is associated with oncocytic thyroid tumours

Oncocytic tumours are characterised by hyperproliferation of mitochondria. We immunohistochemically analysed all enzymes of the oxidative phosphorylation system in 19 oncocytic thyroid tumours. A specific lack of complex I was detected, which was expressed at <5% of the level determined in surrounding non-cancerous tissue

Serological Evidence of Subclinical Transmission of the 2009 Pandemic H1N1 Influenza Virus Outside of Mexico

Background: Relying on surveillance of clinical cases limits the ability to understand the full impact and severity of an epidemic, especially when subclinical cases are more likely to be present in the early stages. Little is known of the infection and transmissibility of the 2009 H1N1 pandemic influenza (pH1N1) virus outside of Mexico prior to clinical cases being reported, and of the knowledge pertaining to immunity and incidence of infection during April-June, which is essential for understanding the nature of viral transmissibility as well as for planning surveillance and intervention of future pandemics. Methodology/Principal Findings: Starting in the fall of 2008, 306 persons from households with schoolchildren in central Taiwan were followed sequentially and serum samples were taken in three sampling periods for haemagglutination inhibition (HI) assay. Age-specific incidence rates were calculated based on seroconversion of antibodies to the pH1N1 virus with an HI titre of 1: 40 or more during two periods: April-June and September-October in 2009. The earliest time period with HI titer greater than 40, as well as a four-fold increase of the neutralization titer, was during April 26-May 3. The incidence rates during the pre-epidemic phase (April-June) and the first wave (July-October) of the pandemic were 14.1% and 29.7%, respectively. The transmissibility of the pH1N1 virus during the early phase of the epidemic, as measured by the effective reproductive number R(0), was 1.16 (95% confidence interval (CI): 0.98-1.34). Conclusions: Approximately one in every ten persons was infected with the 2009 pH1N1 virus during the pre-epidemic phase in April-June. The lack of age-pattern in seropositivity is unexpected, perhaps highlighting the importance of children as asymptomatic transmitters of influenza in households. Although without virological confirmation, our data raise the question of whether there was substantial pH1N1 transmission in Taiwan before June, when clinical cases were first detected by the surveillance network

The Vitamin D Receptor Is a Wnt Effector that Controls Hair Follicle Differentiation and Specifies Tumor Type in Adult Epidermis

We have investigated how Wnt and vitamin D receptor signals regulate epidermal differentiation. Many epidermal genes induced by β-catenin, including the stem cell marker keratin 15, contain vitamin D response elements (VDREs) and several are induced independently of TCF/Lef. The VDR is required for β-catenin induced hair follicle formation in adult epidermis, and the vitamin D analog EB1089 synergises with β-catenin to stimulate hair differentiation. Human trichofolliculomas (hair follicle tumours) are characterized by high nuclear β-catenin and VDR, whereas infiltrative basal cell carcinomas (BCCs) have high β-catenin and low VDR levels. In mice, EB1089 prevents β-catenin induced trichofolliculomas, while in the absence of VDR β-catenin induces tumours resembling BCCs. We conclude that VDR is a TCF/Lef-independent transcriptional effector of the Wnt pathway and that vitamin D analogues have therapeutic potential in tumors with inappropriate activation of Wnt signalling