When epigenetics meets bioengineering—A material characteristics and surface topography perspective

The field of tissue engineering and regenerative medicine (TE/RM) involves regeneration of tissues and organs using implantable biomaterials. The term epigenetics refers to changes in gene expression that are not encoded in the DNA sequence, leading to remodeling of the chromatin and activation or inactivation of gene expression. Recently, studies have demonstrated that these modifications are influenced not only by biological cues but also by mechanical and topographical signals. This review highlights the current knowledge on emerging approaches in TE/RM with a focus on the effect of materials and topography on the epigenetic expression pattern in cells with potential impacts on modulating regenerative biology. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2065–2071, 2018.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144628/1/jbmb33953.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144628/2/jbmb33953_am.pd

Entanglement of excited states in critical spin chians

Renyi and von Neumann entropies quantifying the amount of entanglement in ground states of critical spin chains are known to satisfy a universal law which is given by the Conformal Field Theory (CFT) describing their scaling regime. This law can be generalized to excitations described by primary fields in CFT, as was done in reference (Alcaraz et. al., Phys. Rev. Lett. 106, 201601 (2011)), of which this work is a completion. An alternative derivation is presented, together with numerical verifications of our results in different models belonging to the c=1,1/2 universality classes. Oscillations of the Renyi entropy in excited states and descendant fields are also discussed.Comment: 23 pages, 13 figure

Spectroscopic Study of IRAS 19285+0517(PDS 100): A Rapidly Rotating Li-Rich K Giant

We report on photometry and high-resolution spectroscopy for IRAS 19285+0517. The spectral energy distribution based on visible and near-IR photometry and far-IR fluxes shows that the star is surrounded by dust at a temperature of $T_{\rm {d}}$ $\sim$ 250 K. Spectral line analysis shows that the star is a K giant with a projected rotational velocity $v sin i$ = 9 $\pm$ 2 km s$^{-1}$. We determined the atmospheric parameters: $T_{\rm {eff}}$ = 4500 K, log $g$ = 2.5, $\xi_{t}$ = 1.5 km s$^{-1}$, and [Fe/H] = 0.14 dex. The LTE abundance analysis shows that the star is Li-rich (log $\epsilon$(Li) = 2.5$\pm$0.15), but with essentially normal C, N, and O, and metal abundances. Spectral synthesis of molecular CN lines yields the carbon isotopic ratio $^{12}$C/$^{13}$C = 9 $\pm$3, a signature of post-main sequence evolution and dredge-up on the RGB. Analysis of the Li resonance line at 6707 \AA for different ratios $^{6}$Li/$^{7}$Li shows that the Li profile can be fitted best with a predicted profile for pure $^{7}$Li. Far-IR excess, large Li abundance, and rapid rotation suggest that a planet has been swallowed or, perhaps, that an instability in the RGB outer layers triggered a sudden enrichment of Li and caused mass-loss.Comment: To appear in AJ; 40 pages, 9 figure

Abundances in Stars from the Red Giant Branch Tip to Near the Main Sequence Turn Off in M71: III. Abundance Ratios

We present abundance ratios for 23 elements with respect to Fe in a sample of stars with a wide range in luminosity, from luminous giants to stars near the turnoff, in the globular cluster M71. The analyzed spectra, obtained with HIRES at the Keck Observatory, are of high dispersion (R=35,000). We find that the neutron capture, the iron peak and the alpha-element abundance ratios show no trend with Teff, and low scatter around the mean between the top of the RGB and near the main sequence turnoff. The alpha-elements Mg, Ca, Si and Ti are overabundant relative to Fe. The anti-correlation between O and Na abundances, observed in other metal poor globular clusters, is detected in our sample and extends to the main sequence. A statistically significant correlation between Al and Na abundances is observed among the M71 stars in our sample, extending to Mv = +1.8, fainter than the luminosity of the RGB bump in M5. Lithium is varying, as expected, and Zr may be varying from star to star as well. M71 appears to have abundance ratios very similar to M5 whose bright giants were studied by Ivans et al. (2001), but seems to have a smaller amplitude of star-to-star variations at a given luminosity, as might be expected from its higher metallicity. The results of our abundance analysis of 25 stars in M71 provide sufficient evidence of abundance variations at unexpectedly low luminosities to rule out the mixing scenario. Either alone or, even more powerfully, combined with other recent studies of C and N abundances in M71 stars, the existence of such abundance variations cannot be reproduced within the context of our current understanding of stellar evolution.Comment: AJ, in press (June 2002), 18 figure

Manejo de produtos florestais não madeireiros na Amazônia.

bitstream/item/175558/1/032007-kamukaia-carol.pd

Three-vortex configurations in trapped Bose-Einstein condensates

We report on the creation of three-vortex clusters in a $^{87}Rb$ Bose-Einstein condensate by oscillatory excitation of the condensate. This procedure can create vortices of both circulation, so that we are able to create several types of vortex clusters using the same mechanism. The three-vortex configurations are dominated by two types, namely, an equilateral-triangle arrangement and a linear arrangement. We interpret these most stable configurations respectively as three vortices with the same circulation, and as a vortex-antivortex-vortex cluster. The linear configurations are very likely the first experimental signatures of predicted stationary vortex clusters.Comment: 4 pages, 4 figure

A conserved dimorphism-regulating histidine kinase controls the dimorphic switching in Paracoccidioides brasiliensis

Paracoccidioides brasiliensis and P. lutzii, thermally dimorphic fungi, are the causative agents of paracoccidioidomycosis (PCM). Paracoccidioides infection occurs when conidia or mycelium fragments are inhaled by the host, which causes the Paracoccidioides cells to transition to the yeast form. The development of disease requires conidia inside the host alveoli to differentiate into yeast cells in a temperature-dependent manner. We describe the presence of a two-component signal transduction system in P. brasiliensis, which we investigated by expression analysis of a hypothetical protein gene (PADG_07579) that showed high similarity with the dimorphism-regulating histidine kinase (DRK1) gene of Blastomyces dermatitidis and Histoplasma capsulatum. This gene was sensitive to environmental redox changes, which was demonstrated by a dose-dependent decrease in transcript levels after peroxide stimulation and a subtler decrease in transcript levels after NO stimulation. Furthermore, the higher PbDRK1 levels after treatment with increasing NaCl concentrations suggest that this histidine kinase can play a role as osmosensing. In the mycelium-yeast (M -> Y) transition, PbDRK1 mRNA expression increased 14-fold after 24 h incubation at 37A degrees C, consistent with similar observations in other virulent fungi. These results demonstrate that the PbDRK1 gene is differentially expressed during the dimorphic M -> Y transition. Finally, when P. brasiliensis mycelium cells were exposed to a histidine kinase inhibitor and incubated at 37A degrees C, there was a delay in the dimorphic M -> Y transition, suggesting that histidine kinases could be targets of interest for PCM therapy.CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Brazil)FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/Brazil)Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Unidade Jose Alencar, St Sao Nicolau 210,4 Floor, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Farmaceut, R Sao Nicolau 210, BR-09913030 Diadema, SP, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Unidade Jose Alencar, St Sao Nicolau 210,4 Floor, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Farmaceut, R Sao Nicolau 210, BR-09913030 Diadema, SP, BrazilCNPq: 478023/2013-8FAPESP: 2014/13961-1FAPESP: 2015/09727-6Web of Scienc