41 research outputs found
Synthesis of chiral ferrocenylazines. Negishi cross-coupling or S N H reactions?
Preparation of new hetaryl-containing planar chiral ferrocene by a nucleophilic substitution of hydrogen in azines was performed using a lithium derivative of (S)-ferrocenyl-p-tolylsulfoxide as s nucleophilic reagent © 2013 Pleiades Publishing, Ltd
Synthesis of PlanarChiral Azinylferrocenes
Two synthetic routes to the chiral azinylferrocenes (CAFs) 6, key intermediates for the synthesis of new enantiomerically enriched P,N-ligands, have been compared. The palladium complexes of P,N-ligands have been used as catalysts for allylic alkylation and amination reactions, which proceeds with high enantioselectivity to give substitution products in good yields
Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study
The recovery period after traumatic brain injury (TBI) is often complicated by secondary damage that may last for days or even months after trauma. Two proteins, Hsp70 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were recently described as modulating post-traumatic processes, and in this study, we test them as targets for combination therapy using an inhibitor of GAPDH aggregation (derivative of hydrocortisone RX624) and an inducer of Hsp70 synthesis (the pyrrolylazine derivative PQ-29). The protective effect of the combination on C6 rat glioblastoma cells treated with the cerebrospinal fluid of traumatized animals resulted in an increase in the cell index and in a reduced level of apoptosis. Using a rat weight drop model of TBI, we found that the combined use of both drugs prevented memory impairment and motor deficits, as well as a reduction of neurons and accumulation of GAPDH aggregates in brain tissue. In conclusion, we developed and tested a new approach to the treatment of TBI based on influencing distinct molecular mechanisms in brain cells. © 2022 by the authors.Ministry of Education and Science of the Russian Federation, Minobrnauka: 075-15-2021-683; Russian Science Foundation, RSF: 18-74-10087, 22-13-00298This research was funded by the Russian Science Foundation, research projects #18-74-10087 and #22-13-00298.Rat glioblastoma C6 cells were obtained from the shared research facility “Vertebrate cell culture collection” supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2021-683). Cells were cultured in DMEM/F12 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 100 units/mL penicillin, and 0.1 mg/mL streptomycin (BioloT, St. Petersburg, Russia) at 37 °C and 5% CO.
High-power LEDs based on InGaAsP/InP heterostructures
Hig-hpower ligh-temitting diodes (LEDs) wi
th mesa diameters of 100, 200, and 300
μ
m are devel
oped on the basis of InGaAsP/InP heterostructures. The
mesas are close in shape to a truncated cone with a
generatrix inclination angle of ~45° in the vicinity of
the active region of the LED, with a ring etched around
the mesa serving as a reflector. The emission spectra
and directivity patterns of these LEDs are studied in a
wide range of current densities and it is shown that
radiative recombination is dominant to a current density
of ~5000 A/cm
2
, which makes these structures promising for the development of highpo-er LEDs. An emission power of ~14 mW is obtained in the continuous-wave mode (
I
= 0.2 A,
λ
= 1.1
μ
m), and that of 77 mW,
in the pulsed mode (
I
= 2 A,
λ
= 1.1
μ
m), which corresponds to external quantum efficiencies of 6.2 and
3.4%, respectivel
Influence of divalent cations on the catalytic properties and secondary structure of unadenylylated glutamine synthetase from Azospirillum brasilense
Fully unadenylylated glutamine synthetase (GS) from the endophytic bacterium Azospirillum brasilense Sp245 was isolated and purified. The enzyme was electrophoretically homogeneous and contained strongly bound metal ions, which could not be removed by dialysis. Mn2+, Mg2+, and Co2+ were found to be effective in supporting biosynthetic activity of the A. brasilense GS. Some kinetic properties of Mn2+-activated and Mg2+-activated unadenylylated GS were characterized. Circular dichroism analysis of the enzyme showed that the A. brasilense GS is a highly structured protein: 59% of its residues form alpha -helices and 13% beta -strands. Removal of the metal ions from the A. brasilense GS by treatment with EDTA resulted in alterations in the enzyme secondary structure
Temperature dependence of the threshold current in quantum-well WGM lasers (2.0–2.5 μm)
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