KINEMATICS OF TACTICS IN THE MEN'S 1500 M FREESTYLE SWIMMING FINAL AT THE BEIJING 2008 OLYMPIC GAMES

The purpose of this study was to obtain a knowledge on tactics of long distance swimming at the highest professional level. Eight swimmers - men, finalists of 1500 m distance of freestyle of the Beijing 2008 Olympic Games were investigated. The distribution of velocity of swimming for the entire distance based on 50 m segments was analyzed. Partial, halves and tierces velocities, velocity indexes and linear regression equations were calculated. It was revealed that better swimmers (placed 1 – 4) had their distribution of swimming as ascending line and with very small difference between segmental velocities and that of the entire distance, while the rest of swimmers had descending velocity line and higher dispersion of partial values

Motion analysis of elite Polish soccer goalkeepers throughout a season

The study aims were to determine the distance covered by goalkeepers during matches in the context of game duration and result, to identify the area of their most frequent activity, and to assess goalkeepers' involvement in games finished with a win, draw, or loss. The investigation was based on two innovative tools: the goalkeeper's activity index (GAI) and an analysis of 5-min periods. A video tracking system was used to monitor 17 goalkeepers from Polish National League teams during 15 matches. The GAI was applied to assess their involvement in the game. Elite goalkeepers covered 72.7%, 25.8%, and 2.5% of the distance during the game by walking/jogging, running, and sprinting, respectively. The distances covered in lost, won, and drawn matches turned out similar (mean \ub1 SD: 4800 \ub1 906 m, 4696 \ub1 1033 m, and 4660 \ub1 754 m, respectively). There were no significant differences between the distances covered in the first and second halves. The area of most frequent activity was the middle sector of the penalty area between the goal and penalty area lines. ANOVA results showed that in drawn matches, goalkeepers' activity significantly differed in mean values of the GAI in comparison with that in won and lost games (p = 0.034, p = 0.039, respectively). It was noted that goalkeepers tended to intervene more often in games where their team was winning rather than in those with a losing result. Their direct involvement in defending the goal was the lowest in drawn games

HTRA3 (HtrA serine peptidase 3)

Review on HTRA3 (HtrA serine peptidase 3), with data on DNA, on the protein encoded, and where the gene is implicated

The Pseudoautosomal Regions of the U/V Sex Chromosomes of the Brown Alga Ectocarpus Exhibit Unusual Features

International audienceThe recombining regions of sex chromosomes (pseudoautosomal regions, PARs) are predicted to exhibit unusual features due to their being genetically linked to the nonrecombining, sex-determining region. This phenomenon is expected to occur in both diploid (XY, ZW) and haploid (UV) sexual systems, with slightly different consequences for UV sexual systems because of the absence of masking during the haploid phase (when sex is expressed) and because there is no homozygous sex in these systems. Despite a considerable amount of theoretical work on PAR genetics and evolution, these genomic regions have remained poorly characterized empirically. We show here that although the PARs of the U/V sex chromosomes of the brown alga Ectocarpus recombine at a similar rate to autosomal regions of the genome, they exhibit many genomic features typical of nonrecombining regions. The PARs were enriched in clusters of genes that are preferentially, and often exclusively, expressed during the sporophyte generation of the life cycle, and many of these genes appear to have evolved since the Ectocarpales diverged from other brown algal lineages. A modeling-based approach was used to investigate possible evolutionary mechanisms underlying this enrichment in sporophyte-biased genes. Our results are consistent with the evolution of the PAR in haploid systems being influenced by differential selection pressures in males and females acting on alleles that are advantageous during the sporophyte generation of the life cycle

Repeated co-option of HMG-box genes for sex determination in brown algae and animals

In many eukaryotes, genetic sex determination is not governed by XX/XY or ZW/ZZ systems but by a specialized region on the poorly studied U (female) or V (male) sex chromosomes. Previous studies have hinted at the existence of a dominant male-sex factor on the V chromosome in brown algae, a group of multicellular eukaryotes distantly related to animals and plants. The nature of this factor has remained elusive. Here, we demonstrate that an HMG-box gene acts as the male-determining factor in brown algae, mirroring the role HMG-box genes play in sex determination in animals. Over a billion-year evolutionary timeline, these lineages have independently co-opted the HMG box for male determination, representing a paradigm for evolution’s ability to recurrently use the same genetic “toolkit” to accomplish similar tasks.</p

Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

<p>Abstract</p> <p>Background</p> <p>Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the <it>hsp60-hsp10 </it>bicistronic operon of the animal pathogen <it>Corynebacterium pseudotuberculosis</it>, a Gram-positive bacterium of the class <it>Actinobacteria</it>, which causes caseous lymphadenitis (CLA) in small ruminants.</p> <p>Findings</p> <p>To construct the DNA vaccine, the <it>hsp60 </it>gene of <it>C. pseudotuberculosis </it>was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/<it>hsp60</it>).</p> <p>Conclusion</p> <p>This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.</p

Reversal of the ΔdegP Phenotypes by a Novel rpoE Allele of Escherichia coli

RseA sequesters RpoE (σE) to the inner membrane of Escherichia coli when envelope stress is low. Elevated envelope stress triggers RseA cleavage by the sequential action of two membrane proteases, DegS and RseP, releasing σE to activate an envelope stress reducing pathway. Revertants of a ΔdegP ΔbamB strain, which fails to grow at 37°C due to high envelope stress, harbored mutations in the rseA and rpoE genes. Null and missense rseA mutations constitutively hyper-activated the σE regulon and significantly reduced the major outer membrane protein (OMP) levels. In contrast, a novel rpoE allele, rpoE3, resulting from the partial duplication of the rpoE gene, increased σE levels greater than that seen in the rseA mutant background but did not reduce OMP levels. A σE-dependent RybB::LacZ construct showed only a weak activation of the σE pathway by rpoE3. Despite this, rpoE3 fully reversed the growth and envelope vesiculation phenotypes of ΔdegP. Interestingly, rpoE3 also brought down the modestly activated Cpx envelope stress pathway in the ΔdegP strain to the wild type level, showing the complementary nature of the σE and Cpx pathways. Through employing a labile mutant periplasmic protein, AcrAL222Q, it was determined that the rpoE3 mutation overcomes the ΔdegP phenotypes, in part, by activating a σE-dependent proteolytic pathway. Our data suggest that a reduction in the OMP levels is not intrinsic to the σE-mediated mechanism of lowering envelope stress. They also suggest that under extreme envelope stress, a tight homeostasis loop between RseA and σE may partly be responsible for cell death, and this loop can be broken by mutations that either lower RseA activity or increase σE levels